TGP-2025-27-Leica STELLARIS 8 FALCON-STED Microscope..pdf

SSN # TGP-2025-27- Leica Stellaris 8 Falcon-Sted Microscope Page 1

September 8, 2025

SOLE SOURCE NOTIFICATION (SSN) -TGP-2025-27

Proposed Sole Source Purchase for Leica Stellaris 8 Falcon-Sted Microscope

The University of Wyoming (the University) intends to issue a sole source purchase order to Leica Microsystems for the Leica Stellaris 8 Falcon-Sted Microscope.

Any purchase order resulting from this proposed sole source will be governed by the University of Wyoming’s Terms and Conditions.

The minimum requirements of this purchase are included below:

Technical Features Summary

1. White Light Laser (WLL) with Acousto-Optical Beam Splitter (AOBS®)

• Continuously tunable excitation from 440–790 nm (1 nm precision).

• Enables simultaneous excitation of up to 8 laser lines with independent intensity control.

• Essential for exciting modern engineered fluorescent proteins (GFP variants, YFP, etc.).

2. STED (Stimulated Emission Depletion) Nanoscopy

• True commercial super-resolution system from Leica.

• Achieves resolution <50 nm lateral, <130 nm axial.

• Flexible across visible spectrum with three STED depletion lines (589, 660, 775 nm).

• Includes TauSTED for lifetime-based resolution tuning and background suppression.

3. FALCON (Fast Lifetime Contrast Imaging) & TauSense

• Rapid, photon-counting based FLIM, at least 10× faster than traditional TCSPC.

• TauSense provides lifetime-based species separation, autofluorescence removal, and molecular interaction detection (FRET).

• Enables functional readouts of pH, ion concentration, metabolic status.

4. Power HyD® Detector Family

• Hybrid photon-counting detectors with ultra-low noise and broad sensitivity (410–850 nm).

• Configurations include:

o Power HyD® S: High sensitivity for GFP, CFP, YFP.

o Power HyD® X: Optimized for FALCON and Tau-STED.

o Power HyD® R: Specialized for near-infrared FLIM.

5. Spectral Detection (SP® Detection)

• Prism-based dispersion for efficient spectral separation of overlapping fluorophores.

• Filter-free, adjustable bandwidth detection (5–440 nm) with up to 5 simultaneous channels.

• Superior signal-to-noise ratio and dynamic range for live-cell imaging.

This instrument is the only commercially available microscope that integrates both fluorescence lifetime imaging (FLIM) and true super-resolution imaging (STED nanoscopy) within a single platform. The

SSN # TGP-2025-27- Leica Stellaris 8 Falcon-Sted Microscope Page 2

STELLARIS 8 FALCON-STED will provide an unprecedented resource for investigators studying subcellular localization, protein interactions, and nanoscale dynamics in plants, fungi, bacteria, and single-cell algae.

Any interested party that believes it can comprehensively provide a comparable product as noted above, shall submit a Statement of Capabilities in sufficient detail to determine if the specifications can be met.

University of Wyoming will only accept or consider electronic submissions received in BidNet’s electronic solicitation portal on or before 2:00 PM (MT) on September 15, 2025. Submissions received after this date and time or received outside of the BidNet portal may not be considered. The University shall be the sole judge in reviewing responses with regards to quality, performance, and price.

This notice is issued for informational purposes only and is NOT a request for competitive quotes or proposals. Information received will be considered to determine whether to conduct a competitive solicitation. A determination to not compete this proposed procurement based upon responses to this notice is solely within the discretion of the University.

Any restrictions on the use or inspection of material contained within the statement of capabilities shall be clearly stated in the document itself. The Supplier must state specifically what elements of the statement of capabilities are to be considered confidential/proprietary. Confidential/proprietary information must be readily identified, marked, and separately packaged from the rest of the response. Co-mingling of confidential/proprietary and other information is NOT acceptable. Procurement Services will make a written determination as to the apparent validity of any request for confidentiality. The written decision of Procurement Services will be sent to the Supplier as required.

**Please find more information about doing business with the University here:

How to Do Business with the University of Wyoming https://www.uwyo.edu/procurement/_files/docs/how-to-do-business-with-uw-april-2025.pdf