Statement of Work_RNAseq_144_samples.pdf
PDF 459 KB Posted
- Attached to
- RNA Wheat Sequencing Federal contract opportunity
- Solicitation number
- 12505B23Q0324
About this file
This statement of work outlines RNA sequencing services required for 144 plant and fungal samples. The contractor will perform RNA quality control, directional mRNA library preparation with poly A enrichment, NovaSeq paired-end 150 sequencing, data quality control, and provide fastq data files for all 144 samples via a server download. The related federal contract opportunity is issued by the Department of Agriculture Agricultural Research Service Midwest Area to analyze gene expression in wheat and barley plants inoculated with the fungal pathogen Zymoseptoria tritici at various time points. Key deliverables include RNA sample quality control, library preparation, sequencing, data quality control, and provision of sequencing data files in fastq format to be completed within typically less than 60 days.
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| File | Type | Posted |
|---|---|---|
| 23Q0324- Provisions and Clauses.pdf | ||
| Wage Determination.pdf |
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Text version
STATEMENT OF WORK
(SERVICE)
GENERAL INFORMATION.
1.0 SCOPE OF WORK.
Covered components will include:
2.0 BACKGROUND.
CONTRACTOR REQUIREMENTS.
3.0 TECHNICAL REQUIREMENTS/TASKS.
Service tasks include, but are not limited to:
4.0 GOVERNMENT FURNISHED.
5.0 DELIVERABLES/SCHEDULE.
KEY DELIVERABLES:
Item No. Deliverable Objective Due
6.0 TRAVEL.
7.0 CONTRACTOR’S KEY PERSONNEL.
8.0 SECURITY REQUIREMENTS.
9.0 DATA RIGHTS.
10.0 SECTION 508 – ELECTRONIC AND INFORMATION TECHNOLOGY STANDARDS.
11.0 SECTION 889 OF THE NATIONAL DEFENSE AUTHORIZATION ACT CERTIFICATION
| Text1: Library Preparation and NovaSeq Paired-End Sequencing Service |
| Text2: Contractor will perform RNA sample quality control, directional mRNA library preparation with poly A enrichment for 144 samples, NovaSeq Paired-End 150 sequencing for 144 samples, data quality control, and provide fastq data files via download from the contractor’s server. |
| Text12: 9) |
| Text13: RNA was extracted from plants of the susceptible wheat cultivar Taichung 29 and plants of the barley cultivar Quest that were inoculated with the fungal pathogen of wheat Zymoseptoria tritici. RNA was extracted at 9 time points from controls and inoculated plants having a total of 144 individual samples. We expect to analyze the gene expression from the plants and the fungal pathogen during each biological interaction. These samples will be shipped to the contractor who will then conduct RNA quality control and directional mRNA library preparation, NovaSeq PE 150 sequencing, data quality control, and provide the fastq files for all samples via server download. |
| Text4: 1) RNA quality control for 144 samples |
| Text5: 2) Directional mRNA library preparation with poly A enrichment |
| Text6: 3) NovaSeq Paired-End 150 sequencing for 144 samples |
| Text7: 4) Data quality control for 144 samples |
| Text8: 5) Fastq files containing the final sequencing data |
| Text9: 6) |
| Text10: 7) |
| Text11: 8) |
| Text14: 1) RNA sample quality control |
| Text15: 2) Library preparation of RNA material for 144 samples |
| Text16: 3) Strand-specific RNA-seq with PolyA selection for 144 samples |
| Text17: 4) NovaSeq PE 150 sequencing for 144 samples |
| Text18: 5) Data quality control for sequencing data of 144 samples |
| Text19: 6) Provide sequencing data in fastq file format |
| Text20: 7) Fastq files will be available for download in the contractor's server |
| Text21: 8) |
| Text22: 9) |
| Text23: 10) |
| Text24: 11) |
| Text25: 12) |
| Text26: 13) |
| Text27: Government-furnished support is limited to the RNA extraction that was conducted for 144 samples to be shipped to the contractor. |
| Text29: 2 |
| Text30: 3 |
| Text31: 4 |
| Text32: 5 |
| Text33: 6 |
| Text34: 7 |
| Text35: 8 |
| Text65: None |
| Text67: Not applicable - RNA samples will be sent to the contractor via commercial shipping services without any visits to our location. |
| Text68: We retain the rights to NovaSeq sequencing fastq data files which we will obtain from the contractor’s server. |
| Text66: Contractor’s personnel will be highly trained and successful to perform the RNA sample quality control, directional mRNA library preparation with poly A enrichment, NovaSeq PE 150 Sequencing, data quality control and generation of fastq files containing the sequencing data. |
| Text69: Not applicable |
| Text70: Offeror will represent that: |
(1) It will not provide covered telecommunications equipment or services to the Government in the performance of any contract, subcontract or other contractual instrument resulting from this solicitation.
(2) After conducting a reasonable inquiry, for purposes of this representation, the Offeror represents that It does not use covered telecommunications equipment or services, or use any equipment, system, or service that uses covered telecommunications equipment or services.
| Text29a: |
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| Text35a: |
| Text28a: Fastq data files |
| Text28b: Obtain the sequencing data in files in fastq format from NovaSeq Sequencing via download from the contractor's sever |
| Text30b: |
| Text31b: |
| Text32b: |
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| Text29b: |
| Text35b: |
| Text28: 1 |
| Text29c: |
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| Text34c: |
| Text28c: Typically less than 60 days |
| Text35c: |
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