Statement of Work.pdf

Statement of Work (SOW) Project: Probe design and library kit purchase for sequencing

GENERAL INFORMATION

Scope of Work: Purpose is to design tiling probes for grape powdery mildew based on select transcripts and to purchase library preparation kits and the accompanying supplies. The scope of work includes probe design, probes, capture and library supplies, and technical support for library preparation.

CONTRACTOR REQUIREMENTS

Technical Requirements/Tasks: Design tiling probes with a minimum of 1x tiling coverage for 1500 grape powdery mildew genes with minimal cross compatibility with other organisms. Provide capture and library preparation kits compatible with developed probes for next generation sequencing:

• Tile array options with 1-2x coverage of select genes

• Only baits with a <5 hits should be included in the final probe list to eliminate non specific hybridization

• Probes must be designed using masked genome and transcript files provided by USDA and tested (in silico) against non-target organizations to ensure no cross reactivity

• Library preparation kits for DNA-based next gen sequencing with enough materials to create 250-300 libraries.

• Kits must be compatible with pooled capture sequencing of up to 100 individuals

• Capture reagents must be provided in addition to probes and library preparation kits