Statement of Work.pdf
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- Genotyping Onion DNA Federal contract opportunity
- Solicitation number
- 12505B20R1012731
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Statement of Work (SOW) Project: Onion Genotyping
GENERAL INFORMATION
1.0 Scope of Work: Genotyping of 192 onion DNAs for 250 single nucleotide polymorphisms (SNPs) for genetic mapping of insect resistance.
2.0 Background: Onion populations have been developed that segregate for resistance to thrips, the main insect pest of onion. SNPs are a class of robust genetic markers that are useful for establishing the genetic basis of thrips resistance. These SNPs have been developed by the USDA and DNA sequences to detect them have been published and are openly available. Genotypes at SNPs will be used to produce a genetic map to establish the genetic inheritance of the resistance, which will be useful to onion breeders to develop thrips-resistant cultivars.
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:
o The contractor will use the KASP platform to SNP genotype the onion DNAs. The SNPs are optimized for this platform and sequences necessary to genotype each SNP have been established, resulting in a very high probability of good genotyping results.
DNAs of parents of the segregating families will be included as the positive controls.
o The KASP platform is a widely used genotyping approach, and must be used because the SNPs have been optimized for this platform.
4.0 Government Furnished: The USDA will provide DNAs of 192 onion DNAs of sufficient quality and quantity to complete the genotyping.
5.0 Deliverables / Schedule:
o Deliverable will be 250 SNP genotypes for 192 onion DNAs and the schedule is open to discussion, however we would like the results by December 1 2020.
o The contractor is required to provide written progress reports if the genotyping is not completed by December 1, 2020.
Key Deliverables Item No.
Deliverable
Objective
Due
1 Genotypes for 250 SNPs across 192 onion DNAs
Genetic analysis of insect resistance in onion.
December 1 2020
6.0 Travel: None
7.0 Contractor’s Key Personnel: Experience with KASP platform is required.
8.0 Security Requirements: None
9.0 Data Rights: Genotypic data is to provide in the form of an excel spreadsheet with genotypes for all SNPs across all DNAs. The data must be confidential and provided only to the USDA Vegetable Crops Research Unit, Madison WI.
10.0 Section 508 – Electronic and Information Technology Standards: None special.
| CONTRACTOR REQUIREMENTS |
| 3.0 Technical Requirements/Tasks: |
| o The contractor will use the KASP platform to SNP genotype the onion DNAs. The SNPs are optimized for this platform and sequences necessary to genotype each SNP have been established, resulting in a very high probability of good genotyping results. ... |
| 6.0 Travel: None |
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