Statement of Work.doc
DOC document 77 KB Posted
- Attached to
- RNA Sequencing Federal contract opportunity
- Solicitation number
- 1232SA25Q0327
About this file
The document is a Statement of Work (SOW) for mRNA sequencing services issued by the USDA Agricultural Research Service (ARS) at the U.S. National Poultry Research Center in Athens, GA. The solicitation (Number 1232SA25Q0327) seeks a contractor to perform comprehensive mRNA sequencing and bioinformatics analysis on 354 chicken tissue samples, with detailed technical requirements including RNA sample quality control, library preparation, Illumina sequencing, and advanced bioinformatics analyses such as gene expression quantification, alternative splicing analysis, pathway enrichment, and protein interaction analysis.
The procurement is a 100% small business set-aside with a NAICS code of 541380 and a small business size standard of $19.0 million. Quotes are due on 8/11/2025 at 2:00 PM PT, to be submitted via email to Elizabeth Wilson, with evaluation based on best value considering price, technical capability, and past performance. The contract will be awarded as a firm fixed-price commercial item using FAR Part 13 procedures, with the primary objective of generating data to support publications in peer-reviewed scientific journals.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Statement of Work_RNAseq_ketosis_v2.doc | DOC document | |
| Wage Determination 2015-4469 Rev 27 05222025.pdf | ||
| Contract Terms and Conditions.docx | DOCX document |
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Statement of Work (SOW) mRNA sequencing of various chicken tissue samples for USDA-ARS, USNPRC, QUALITY & SAFETY ASSESSMENT RESEARCH UNIT
GENERAL INFORMATION
Scope of Work: This contract is located at the USNPRC at 950 College Station Road Athens GA, 30605. A company/analytical service will conduct mRNA sequencing and provide bioinformatics analysis of chicken tissue samples provided by ARS. The company/analytical service will perform all experiments at their location. The purpose of this SOW is to acquire data that will support publications in peer reviewed journals. Analyses and deliverables include:
Plant and animal eukaryotic mRNA-seq with reference (WBI)
- RNA sample QC
- mRNA library preparation (poly A enrichment)
- Illumina sequencing platform (e.g. NovaSeq, HiSeq, etc.)
- 6 G raw data per sample
- 354 samples
- Standard bioinformatics analyses including:
- Novel Gene Prediction
- Alternative Splicing Analysis
- SNP/InDel Analysis
- Gene Expression Quantification
- Differential Expression Analysis
- GO Enrichment Analysis of Differentially Expressed Genes (DEGs)
- GSEA Enrichment Analysis of Expressed Genes
- KEGG Pathway Enrichment Analysis
- Protein Protein Interaction Analysis
- Reactome Pathway Enrichment Analysis o Contractor Furnished Items and Services: GENERAL. Contractor shall furnish everything required to perform this Statement of Work.
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