Statement of Work_RNAseq_ketosis_v2.doc
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- Attached to
- RNA Sequencing Federal contract opportunity
- Solicitation number
- 1232SA25Q0327
About this file
The Statement of Work (SOW) details a mRNA sequencing project for the USDA Agricultural Research Service's Quality & Safety Assessment Research Unit located in Athens, GA. The contract requires an analytical service to perform comprehensive RNA sequencing on 354 chicken tissue samples, including multiple sophisticated bioinformatics analyses such as novel gene prediction, alternative splicing analysis, SNP/InDel analysis, gene expression quantification, differential expression analysis, pathway enrichment studies, and protein interaction analysis. The sequencing will be conducted using an Illumina sequencing platform, generating 6G of raw data per sample.
The associated federal contract opportunity (Solicitation Number: 1232SA25Q0327) is a 100% small business set-aside with a size standard of $19.0 million under NAICS code 541380. Quotes are due on 8/11/2025 at 2:00 PM PT, with questions accepted until 8/4/2024. The award will be based on best value, evaluated across three primary factors: price, technical capability, and past performance. Interested offerors must submit a firm fixed-price quotation, provide their Sam.gov Unique Entity ID, and demonstrate technical capability to meet the Statement of Work requirements. The contract will be administered by Elizabeth Wilson at Elizabeth.Wilson2@usda.gov.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Wage Determination 2015-4469 Rev 27 05222025.pdf | ||
| Contract Terms and Conditions.docx | DOCX document | |
| Statement of Work.doc | DOC document |
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Statement of Work (SOW) mRNA sequencing of various chicken tissue samples for USDA-ARS, USNPRC, QUALITY & SAFETY ASSESSMENT RESEARCH UNIT
GENERAL INFORMATION
Scope of Work: This contract is located at the USNPRC at 950 College Station Road Athens GA, 30605. A company/analytical service will conduct mRNA sequencing and provide bioinformatics analysis of chicken tissue samples provided by ARS. The company/analytical service will perform all experiments at their location. The purpose of this SOW is to acquire data that will support publications in peer reviewed journals. Analyses and deliverables include:
Plant and animal eukaryotic mRNA-seq with reference (WBI)
- RNA sample QC
- mRNA library preparation (poly A enrichment)
- Illumina sequencing platform (e.g. NovaSeq, HiSeq, etc.) or equal quality sequencing platform
- 6 G raw data per sample
- 354 samples
- Standard bioinformatics analyses including:
- Novel Gene Prediction
- Alternative Splicing Analysis
- SNP/InDel Analysis
- Gene Expression Quantification
- Differential Expression Analysis
- GO Enrichment Analysis of Differentially Expressed Genes (DEGs)
- GSEA Enrichment Analysis of Expressed Genes
- KEGG Pathway Enrichment Analysis
- Protein Protein Interaction Analysis
- Reactome Pathway Enrichment Analysis o Government Furnished: No Government-furnished property will be provided.
Contractor Furnished Items and Services: GENERAL. Contractor shall furnish everything required to perform this Statement of Work.
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