Specifications.pdf

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Attached to
RNA sequencing Federal contract opportunity
Solicitation number
12805B23Q0476
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Plains Area

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CLAUSES AND PROVISIONS - 12805B23Q0476.pdf PDF

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Specifications:

Must meet or exceed the following:

Scope of Work

Sunflower Basal Stalk Rot RNA sequencing

Vendor will prepare RNA sequencing (RNAseq) libraries for 72 samples of sunflower root total RNA to be provided by USDA-ARS and conduct appropriate quality control assessments for submitted samples and RNAseq libraries. Vendor will sequence prepared RNA libraries to a minimum average read depth of 20 million reads (3Gb data) per sample. Vendor will conduct basic data quality control to include demultiplexing and filtering of low-quality reads or reads containing adapter sequence, no other data analysis is required. Vendor will deliver RNAseq read data in FASTQ file format to USDA-ARS either on physical media (external hard drive) or by providing a secure file download. Vendor will deliver RNAseq data within 90 business days after confirmation of submitted RNA sample quality.

CLIN Description Unit Quantity

0001 RNA library preparation and sequencing

Sample 72

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