SOW_PR0044031882_Analysis_Fish_Liver_Samples_2.pdf

PR0044031882 Analysis Fish Liver Samples

STATEMENT OF WORK

1. BACKGROUND

The U.S. Geological Survey (USGS) conducts long-term monitoring projects on the Snake River to estimate abundance, growth, and survival of juvenile fall Chinook salmon migrating through Lower Granite Reservoir. A portion of these juvenile salmon remains in the reservoir for extended periods, during which they feed primarily on zooplankton and invertebrates.

In recent years, harmful algal blooms (HABs) have occurred during summer months in Lower Granite Reservoir. Prior research indicates that planktivorous fish accumulate higher levels of microcystins (MCs) than carnivorous fish and that liver tissue is a primary target organ for toxin accumulation.

This requirement represents a continuation of previously awarded analytical work supporting ongoing USGS monitoring efforts.

The 3-methoxy-2-methyl-4-phenylbutyric acid (MMPB) method is required to detect total microcystins (free and bound forms), as alternative screening methods may under-detect bound microcystins in liver tissue.

2. OBJECTIVE

Analyze fish liver tissue samples submitted by USGS to determine concentrations of total microcystins using the MMPB analytical method and to provide analytical results to USGS in an electronic format.

3. SCOPE OF WORK

The contractor shall analyze fish liver tissue samples provided by USGS in accordance with this Statement of Work.

3.1 Sample Quantity

Up to 80 fish liver samples for analysis.

3.2 Sample Preparation and Submission (Government-Furnished Items)

USGS will:

• Prepare liver tissue samples in accordance with contractor-provided procedures

• Individually package and label each sample with a sample identification number and sample type (liver tissue)

• Ship samples overnight on dry ice to the contractor’s designated laboratory

PR0044031882 Analysis Fish Liver Samples

4. CONTRACTOR REQUIREMENTS

4.1 Analytical Method

The contractor shall analyze all submitted fish liver samples using the MMPB method to detect total microcystins, including free and bound forms.

5. DELIVERABLES

5.1 Analytical Results

The contractor shall provide an electronic Excel spreadsheet or PDF document containing, at a minimum:

• Sample identification number

• Concentration of microcystins detected for each sample

• Units of measurement

5.2 Delivery Timeline

The results will be provided to the USGS, and an invoice will be submitted by 03/31/2026.

6. PLACE OF PERFORMANCE

Contractor’s laboratory facilities.

7. GOVERNMENT-FURNISHED PROPERTY / INFORMATION (GFP/GFI)

The Government will furnish fish liver tissue samples as described in Section 3.

8. ACCEPTANCE CRITERIA

Deliverables will be accepted when all submitted samples have corresponding analytical results and results are provided in the required electronic format.