SOW.docx
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- Attached to
- NEXT GENERATION SEQUENCING ITEMS
- Solicitation number
- VEID260013_1301365570
About this file
This is a Performance Work Statement (PWS) for the procurement of next-generation sequencing (NGS) reagents and consumables by the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) Virology Department. The procuring activity is the Department of the Navy Naval Supply Systems Command, with delivery required to NAMRU SOUTH in Bellavista, Callao, Peru.
The required products are Illumina-compatible sequencing and library preparation kits with specific quantities: two PhiX Control v3 kits (Cat. # FC-110-3001), ten MiSeq Reagent Kits v3 600-cycle (MS-102-3003), three Nextera XT DNA Sample Preparation Kits (FC-131-1096), one Nextera XT Index Kit v2 Set A with 96 indexes for 384 samples (FC-131-2001), six MiniSeq High Output Reagent Kits 300-cycle (FC-420-1003), and one Nextera XT Index Kit v2 Set B with 96 indexes for 384 samples (FC-131-2002). The library preparation kits must support low DNA input of approximately 50 nanograms, include enzymatic fragmentation, size selection, end-repair, A-tailing, and ligation in a single reaction, and permit dual indexing at both 5' and 3' ends. Sequencing kits must support more than 150 cycles for paired-end libraries with high-quality performance yielding at least 2.0 gigabases per run with Q30 values and include an internal control for run validation. All materials require cold chain maintenance, and the vendor must communicate expiration dates to end users prior to shipment pending their approval. Delivery is required upon award signature, with no hazardous materials identified in the procurement.
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PERFORMANCE WORK STATEMENT
1. SCOPE
The laboratory of the Virology Department of the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is looking to purchase reagents to prepare libraries using human samples that later will be sequenced in the MiSeq and MiniSeq platforms developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command.
1.1. BACKGROUND
The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.
2. REQUIREMENTS
2.1. Location of work / Place of delivery
Shipment to NAMRU SOUTH – Callao, Peru
2.2. Period of performance / Delivery time
In accordance with deliverables schedule.
2.3. Deliverables schedule (if applicable)
Materials should be shipped upon award signature.
2.4. Hazardous material category.
None of the items are hazardous materials.
2.5. List of products
| Cat. # |
| Item |
| Quantity |
| FC-110-3001 |
| PhiX control v3 kit |
| 2 |
| MS-102-3003 |
| MiSeq Reagent Kit v3 (600-cycle) |
| 10 |
| FC-131-1096 |
| Nextera XT DNA Sample Preparation Kit |
| 3 |
| FC-131-2001 |
| Nextera XT Index Kit v2 Set A (96 indexes, 384 samples) |
| 1 |
| FC-420-1003 |
| MiniSeq High Output Reagent Kit (300-cycles) |
| 6 |
| FC-131-2002 |
| Nextera XT Index Kit v2 Set B (96 indexes, 384 samples) |
| 1 |
2.6. Applicable Standards
These reagents are very time-sensitive and have limited expiration dates, reason for which, vendor should inform final users of expiration dates as soon as they become available and request their approval prior to shipment.
2.7. Special Requirements
All materials require cold chain.
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