SOW.docx

Statement of Work for Real-Time PCR System Project Overview This project aligns with the objectives to conduct real-time PCR analyses for research at the Abdel-Latif laboratory. The acquisition of the Real-Time PCR System will enhance the lab's capabilities for precise gene expression analysis, genotyping, and pathogen detection.

Purpose of the Project The primary objective is to implement a robust and versatile real-time PCR platform that can significantly enhance the efficiency and innovation of our discovery research, with specific emphasis on high-sensitivity quantification of gene expression, genotyping, and multiplex analysis capabilities.

Background The research projects require a sophisticated real-time PCR platform for accurate quantitative measurements and analysis of DNA and RNA targets. The Real-Time PCR System provides the technological capabilities essential for achieving the molecular analysis goals outlined in ongoing and planned research initiatives.

Scope and Equipment Specifications The vendor will provide the Real-Time PCR System with the following specifications:

· 96-well or 384-well block configuration with fast protocol capability

· White LED excitation source with OptiFlex technology (6 decoupled channels for 96-well or 5 coupled channels for 384-well format)

· VeriFlex Block technology with 6 independent temperature zones

· Integrated touchscreen interface with cloud connectivity

· Compliance with 21 CFR Part 11 guidelines

· Completion of 40-cycle runs in under 30 minutes (96-well) or 35 minutes (384-well)

· Factory calibration for multiple dyes including FAM, SYBR Green, VIC, NED, ABY, JUN, Mustang Purple, TAMRA, Cy5, and ROX Tasks and Associated Deliverables Equipment Delivery and Installation Deliverables:

· Delivery of the Real-Time PCR System to designated laboratory location

· Installation setup documentation

· RNase P verification plate for performance verification

· IT checklist for streamlining internet connectivity Training and Knowledge Transfer Deliverables:

· Orientation session provided by a trained technical scientist or through eLearning modules

· User training materials and access to support resources

· Documentation for system operation and maintenance System Validation and Setup Deliverables:

· Instrument qualification (IQ), operational qualification (OQ), and instrument performance verification (IPV) services

· Verification of optical and thermal accuracy

· Documentation of system performance specifications Reporting Requirements

· Installation and qualification report

· Performance verification documentation

· System specifications and validation documentation

· Warranty information and service contract details Warranty and Support

· One-year limited warranty from the date of installation (or 15 months from shipment)

· Optional post-warranty service contracts with next-business-day on-site repairs

· Telephone technical support and field applications assistance By signing this Statement of Work, the parties agree to adhere to the objectives, tasks, deliverables, and reporting requirements as specified above.