SOW DORIC Fiber Photometry System.docx

Sioux Falls Health Care System 2501 W 22nd Street Sioux Falls, SD 57105 Project: SF Research Activation - DORIC Fiber Photometry system

STATEMENT OF WORK - Functional/Performance/Specification (Brand Name or Equal)

1.0 General Scope

This requirement includes warranty services. Sioux Falls VA Health Care System requires the following Brand Name or Equal items: DORIC Fiber photometry system. The contractor shall deliver all system materials, equipment, and assembly/installation guides.

2.0 Performance Requirements

The contractor shall provide and be responsible for technical assistance, development, and generation for final specifications.

Delivery to the Research building, a one level building located on the Sioux Falls VA Health Care System campus at 2501 West 22nd Street, Sioux Falls, SD 57105. Ground level unloading is required. Installation window to be arranged during the activation timeframe with a tentative installation date no earlier than May 2026. VA will provide a schedule update at the time of award.

Duty hours will be 8am to 430pm, Monday thru Friday excluding holidays. The Government recognized US holidays are: New Year’s Day, Martin Luther King, Jr. Day, Presidents Day, Memorial Day, Juneteenth, Independence Day, Labor Day, Columbus Day, Veterans Day, Thanksgiving Day and Christmas Day. If the holiday falls on Sunday, it is observed on Monday.

3.0 PRODUCT (Functional/Performance/Specification (Brand Name or Equal)) This requirement includes meeting Brand Name or equal to the DORIC Fiber Photometry system. The system includes Behavior & Bundle Photometry Console (BBC300), Fiber Photometry with Fluorescence Mini Cubes, Fiber Photometry with Rotary Fluorescence Mini Cube, Bundle-imaging Fiber Photometry, Wireless Photometry, Rotary Bundle-imaging Fiber Photometry, FluoPulse™ system, Doric Neuroscience Studio V6 software, Danse™ software, and Fiber Photometry Accessories.

Fiber photometry monitors neuronal activity in the brain of freely moving behaving animals that helps correlate brain functioning with animal behavior. It uses implanted fiber-optic cannula to deliver excitation light to a group of neurons labeled with genetically encoded calcium indicators and detect their calcium-related fluorescence fluctuations. While it lacks the cellular/spacial resolution of a miniature fluorescence microscope, fiber photometry uses a relatively simple setup to record fluctuations in the calcium activity of a group of neurons in behaving animals. A smaller cannula's fiber diameter is better suited for deep tissue neuronal activity monitoring than a somewhat larger lens of miniature fluorescence microscopes.

Optogenetics uses light to control the activity of specific neurons that have been genetically modified to express light sensitive proteins. Allows researchers to turn neurons on or off based on cell type, enabling causal investigation of neural circuit function. Used to test the role of specific neural pathways in complex behaviors like aggression, anxiety, and sleep. Allows for precise control over when and where neural activity is manipulated.

Integrating fiber photometry and optogenetics using specialized software allows for simultaneous recording of neural activity and optogenetic stimulation in freely moving animals. Allows researchers to correlate specific neural activity patterns with specific behaviors. Enables the design of more complex and comprehensive experiments to probe neural circuits.

Sioux Falls VA Health Care System requires the following:

A. QTY 1: Behavior & Bundle Photometry Console

· Must have at least 4 x USB 3.0 ports

· Must have at least 4 x Camera Trigger ports

· Must have at least 4 x Analog Outputs (for light source control)

· Must have at least 8 x Digital Input/Output (DIO) ports

· Must have at least 24 additional Digital ports (8x inputs/outputs, 8x inputs, 8x outputs) with the extension board

B. QTY 1: 4 laser diodes Fiber Light Source - 4x 638nm - 80 mW, LDFLS-4CH and 4x 450nm - 75 mW, LDFLS-4CH

· Must meet the following laser diode and fiber light criteria:

· Optical Fiber: Multimode 50 μm

· Power stability: < 1% over 1 hour with the following Operating Modes: OFF, Continuous, External TTL, External Analog, Computer (USB)

· TTL Modulation: HI > 2.8 V, LOW < 2.3 V

· Analog Modulation: 0 - 5 V (80 mA / V and 75mA/ V)

· Rise / Fall time: < 10 µsec

· Max modulation frequency: 10 kHz

C. QTY 4: Mono Fiberoptic Patchcord, MFP_200/220/LWMJ-0.22_1m_FC-FCA

· Must meet the following Fiber-optic capability: 200/220/900-0.37 or Core (μm): 200, Cladding (μm): 220, Buffer: 245, Jacket: 900, NA: 0.37

D. QTY 1: Branching Box for HD9 pattern - 9 FC inputs, 1 HD9 output

· Must have at least 9 input ports

· Must be compatible with FC Input Optical Connector

· Must be compatible with HD9 Output Optical Connector

· Must have the following dimension, 211 x 94 x 45 mm

E. QTY 1: Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics

· Must have a wavelength range of 350 to 1100 nm

· Must have a field of view (FOV) of Ø3 mm

· Must have an objective numerical aperture (NA) of 0.40

· LEDs Excitation Uniformity must be 80% over FOV

· Optogenetics Excitation Uniformity must be 65% over FOV

· Optogenetics Crosstalk between fibers must be >31 dB

· Must have optical fiber compatibility with the following fiber optic core diameter 200 or 400 µm and NA 0.37

· Must be able to measure spectral bandwidth within the following 3 excitation and 2 emission ranges:

· Excitation #1: 405-410, 410-415, 421-433 or 433-445nm

· Excitation #2: 460-490 nm

· Excitation #3: 555-570 nm

· Emission #1: 500-540 nm

· Emission #2: 580-600 nm

· Optical filter attenuation must be > Optical Density (OD) 5 outside band

· Must be compatible with HD7, HD9 or HD19 Optical fiber connectors.

· Dimensions must be 220mm x 220mm x 59mm

· Must have a CMOS (Complementary Metal-Oxide Semiconductor) image sensor

· Must provide 1024x1024 px resolution

· Must provide Pixels size down to 5.86 µm x 5.86 µm

· Must have a frame rate up to 60 Hz

· Must have a USB 3.0 Computer interface.

F. QTY 1: High Density Fiberoptic Patch cord - Low Autofluorescence

G. QTY 8: Ferrule Connector (FC)/Ferrule Connector (FC) Mating Adapter – Round

H. QTY 8: Fiberoptic Rotary Joint, pigtailed with 400 um, NA0.37 fibre, 1.0m input, 0.15m output, optimized for fiber photometry

I. QTY 8: Gimbal holder for FRJ 1x1_PT-G2 - ID 19 mm

· Must have 96 mm x 69 mm x 25 mm dimensions

J. QTY 8: Mono Fiberoptic Patchcord - Low AutoFluorescence

· Must have the following Fiber-Optic Codes to ensure system compatibility

· Optical Fiber Type: Silica/Silica

· Core: Glass

· Cladding: Glass

· Numerical Aperture: 0.22-0.37

· Flexibility: Good

· Auto-Fluorescence: Excellent

· Core Diameter (μm): 400

· Cladding (μm): 440

· Buffer (μm): 470

· NA: 0.37

· Must have the following Jacket Codes to ensure system compatibility.

· Composition Code: 1100

· Description: Hytrel loose tube 1.1 mm

· Color: Black

· OD (mm): 1.1

· Linear Mass (g/m): 0.5

· Must be compatible with the following Connectors:

· 1st Connector Code: FCM (Flexible metal conduit)

· 1st Connector Description: FC Connector with Metal Ferrule

· 2nd Connector Code: MF1.25(F)

· 2nd Connector Description: Metal Ferrule OD 1.25 mm with Flange

K. QTY 10: Zirconia Sleeve 1.25 mm with black cover

· Must have a Ferrule Diameter of 1.25mm

L. QTY 30: Mono Fiberoptic Cannula

· Fiber-Optic must be made from Silica/Silica material

· Fiber-Optic type must be 400(μm) – NA 0.37

· Fiber Lenth must 5.0mm

· Receptacle must be a male to female (MF) 1.25

· Fiber tip must be flat (FLT)

M. QTY 2: Stereotaxic Cannula Holder 1.25 mm

· Ferrule diameter must be 1.25mm

· Rod diameter must be 6.3 mm

N. QTY 1: CamLoop with RGB camera and Wide-Angle lens, Behavior tracking System for closed loop stimulation

· Must be able to provide 1440 x 1080 px (1.6MP) resolution

· Must have dynamic range of 12 bits

· Must provide gain range of 0 to 48 dB

· Must be able to provide a Frame rate of at least 60 fps at full resolution

· Sensor format: 1 / 2.9''

· Must have a USB 3.0 Interface

· Minimum exposure of 1 ms or less

· Lens must be mounted using C-mount or CS-mount

O. QTY 1: Danse™ 3-year subscription, Data Analysis Software, 1 license

P. QTY 1: Gentec-EO Pronto-SI Power Meter + SM1 Treaded adaptor + 2.5mm receptacle + 1.25mm, receptacle

Q. QTY 1: High performance workstation set up for Doric's systems

· Must have an Intel Core i9 13th gen. 24-core processor or better

· Must have a minimum RAM of 2x32GB DDR5 5600

· Must have GeForce RTX 3060 12GB DDR6 or better graphic card to accurately capture and measure video files.

· Must have SSD 4TB NVMe + Hard Drive 4TB or better to support the software and system.

· Must be Win11 Pro compatible to run the system software.

R. QTY 1: Assisted 2x2 Fiber-optic Rotary Joint, 400 um NA0.37 fibre, 1m input, optimized for fiber photometry – Part # AFRJ_2x2_PT_400-0.37

S. QTY 1: Base Station for wireless fiber photometry system

· Must have a transmission range up to 2 meters

· Must be able to accommodate 4 headstages

· Must operate within 2.4 - 2.5 GHz wireless frequency band.

· Base Station Interface must be compatible with HDMI (to NC500) or USB-B (to BBC300)

T. QTY 1: Wireless Photometry Headstage - ferrule 2.5 mm - 1 integrated photodetector and 2 integrated LEDs. With a 40mAh battery

U. QTY 1: Battery Charger for wireless fiber photometry - Part # WiFP_CHRG

· Must Interface with a USB-C

· Power Supply: 1 meter cable USB-C to USB-A

· Must have a capacity of 4 headstages

V. QTY 10: Multi Fluid Injections Cannulas, OmFC_ZF1.25_400/470-0.37_5.0mm_FLT_4.9mm_PLGS

W. QTY 10: OmFC Fluid Injector – Part # FI_OmFC-ZF_140/190_5.1mm

X. QTY 10: Mono Fiberoptic Cannula– Part # MFC_400/430-0.37_5mm_ZF2.5_FLT

Y. QTY 1: Co-Extruded PE/PVC Tubing for HRJ-OL – Part# HRJ-OL _BTCOEX_25Ga_clear (2 meters)

Warranty Information – 1 Year Warranty or Manufacturer’s Warranty – Whichever is greater.