SOW Cell Line Purchase 25 Aug.pdf

Statement of Work Cell Line Expressing Human Voltage-gated Sodium Channels

25 August 2022

RFQ # W9ZLK-22-Q-0062

1. PROJECT TITLE: Purchase of Cell Line Expressing Human Voltage-gated Sodium Channels.

This is a solution to measure the effects of synthesized conotoxins on its respective target.

2. BACKGROUND:

A cell line is described as a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. Utilizing a cell line allows for investigation of specific targets that are typically difficult to assess in vivo, such as ion channels. Many chemical warfare agents affect the structure and function of ion channels.

Growing cell lines that express specific ion channels allows DEVCOM CBC investigators to test a library of compounds and characterize the change in activity in a manner that was specifically designed for electrophysiological assays, such as the Sophion Q-Patch II Automated Patch Clamp system which is currently in use in the Threat Agent Sciences Division of R&T Directorate. This also reduces the number of animals needed to understand the effects of these agents on ion channel activity. DEVCOM CBC investigators can then compare potency and efficacy of chemical agents to benchmark compounds to rank and/or prioritize our chemical threat list.

3. SCOPE:

The scope of this contract is to purchase required cell lines for the associated project goal of measuring current through ion channels of individual cells when exposed to chemical agent. This will contribute to better risk assessments for chemical threat agents.

4. SPECIFICATIONS (generic description) NAICS Code- 325414 - Biological Product (except Diagnostic)

TWO ITEMS:

- Recombinant Stable Cell Line, hNav 1.4; Quantity: 2 vials; Amount per Vial: between two (2) and six (6) million cells (or minimum of 2 million cells/vial)

AND

- Recombinant Stable Cell Line, hNav 1.2; Quantity: 2 vials; Amount per Vial: between two (2) and six (6) million cells

5. REQUIREMENTS/SPECIFICATIONS:

-Cell line should be rigorously and continuously validated for accurate pharmacology with screening and profiling services used to screen millions of compounds

-There should be flexibility in the cell line backgrounds, including HEK, CHO, U2OS, 1321N1 and others

-Cell lines should have confirmed stability past 10 passages

-Cell line should be characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques

-Cell lines should be tested and found free of microbial contamination

-There should be additional function (pharmacological and electrophysiological) validation on multiple platforms available upon request

-There should be direct technical support offered for cell lines for manual patch-clamp and automated patch-clamp technology; cells should be able to be grown and tested in parallel with the customer to troubleshoot any cell culture or expression issues, and to assist customers in ion channel in electrophysiology assay design.

-A full-time electrophysiologist with at least 25 years of experience should be available to assist with assay design and troubleshooting.

6. DELIVERY SCHEDULE: 90 Days from date of award

7. DELIVERY LOCATION:

DEVCOM CBC

Building E3150 8938 North Kings Creek Road

APG, MD 21010-5424

8. INSTALLATION: N/A

9. GOVERNMENT-FURNISHED EQUIPMENT AND INFORMATION: N/A

10. PLACE OF PERFORMANCE

DEVCOM CBC

Building E3150, 8938 North Kings Creek Road

APG, MD 21010-5424

11. SECURITY: N/A