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This Statement of Work (SOW) specifies requirements for procuring a flow cytometer for NASA to detect and measure physical and chemical characteristics of cell and particle populations.

The required flow cytometer must feature four lasers: Violet (405nm/80mW), Blue (488nm/50mW), Yellow Green (561nm/30mW), and Red (638nm/50mW). Key specifications include fixed integrated optics and quartz flow cell design with >1.3 numerical aperture, flow cell dimensions of 420μm x 180μm internal diameter, and scatter resolution capabilities of <300nm for Blue Side Scatter and 80nm polystyrene particles for Violet Side Scatter. The system must achieve carryover rates of <1.0% in Single Tube Format and <0.5% in Plate Loader Format. Physical requirements specify dimensions of 42.5cm x 42.5cm x 34cm for the cytometer and 14cm x 35.6cm x 35.6cm for tanks/holder, with a cytometer weight of 23.4kg. The unit must operate on 100-240V power drawing 150-250W, within a temperature range of 15-27°C non-condensing.

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Statement of Work

Objective/Requirement:

Purchase flow cytometer to detect and measure the physical and chemical characteristics of a population of cells or particles.

Laser Specs:

Laser Wavelength Power

Violet 405 nm 80 mW

Blue 488 nm 50 mW

Yellow Green 561 nm 30 mW

Red 638 nm 50 mW

Other Specs

Fixed integrated optics and quartz flow cell design with >1.3numerical aperture.

Flow Cell dimensions: 420 μm x 180 μm internal diameter

SCATTER RESOLUTION

Blue (488 nm) Side Scatter Resolution: <300 nm

Violet (405 nm) Side Scatter Resolution (VSSC): 80 nm polystyrene particles

CARRYOVER

Single Tube Format: < 1.0%

Plate Loader Format: < 0.5%

DIMENSIONS (W X D X H)

Cytometer

42.5 cm x 42.5 cm x 34 cm

Tanks and Holder

14 cm x 35.6 cm x 35.6 cm

WEIGHT

Cytometer: 23.4 kg

POWER SPECIFICATIONS

Voltage: 100–240 V Power: 150 -250 W

OPERATING TEMPERATURE NON-CONDENSING

15-27 °C

File details come from the government source that posted it. Updated .