Salient_Characteristics_1.pdf

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eDNA AUTOSAMPLER
Solicitation number
DOIGFBO260037
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This document outlines the salient characteristics and technical specifications for an autonomous eDNA water sampling platform. The platform must collect, filter, and preserve a minimum of 30 eDNA water samples for up to 3 months without human intervention. Key operational requirements include a UV-based system to minimize biofouling, autonomous metadata logging capabilities, and a design that minimizes contamination risks both at the sampling site and during laboratory transport. The platform must be field-serviceable in less than 20 minutes by a single operator and feature reusable, decontaminable filter sample holders compatible with 47-millimeter diameter filters in various materials (PES, GF, MCE) and different preservation solutions (RNALater, EtOH).

Additional technical specifications require water volume accuracy of at least 95 percent for sample volumes of 2 liters or less, land-based deployment from shore locations, compact dimensions allowing portability by one to two people, and battery-powered operation with swappable battery packs. The sampling rate must be user-definable and programmable to accommodate varying operational needs. Vendors must provide evidence of validation and testing demonstrating the platform's ability to meet these specifications.

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Salient Characteristics

This platform must meet or exceed the following specifications:

• Collect, filter, and preserve 30 or more eDNA water samples for up to 3 months autonomously

• UV-based system to minimize biofouling

• Logs sampling metadata

• Easy to field service, requiring minimal time (< 20 min) and personnel (1 person)

• Design minimizes contamination risk at the site and also when samples are being transported to the laboratory

• Filter sample holders can be decontaminated and reused multiple times

• Can accommodate different 47-mm diameter filter types (e.g., PES, GF, MCE)

• Can accommodate different sample preservation solutions (e.g., RNALater, EtOH)

• Water volume accuracy of ≥ 95% for ≤ 2 L sample volumes

• Land-based, can be deployed from shore

• Relatively compact and can be carried by 1-2 people

• Run off of battery in the field, swappable battery pack

• Sampling rate is user-definable and programmable

• Evidence of validation and testing

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