S02 Jackson Mold - AEC_138.22_VA Regional Office Building Mold Report.pdf

AEC

Advanced Environmental Consultants, Inc.

Project Managers and Scientists

P. O. Box 16847 775 N. President Street Jackson, MS 39236-6847 Jackson, MS 39208

Phone: (601) 362-1788 Fax: (601) 362-3967 Email: aecinc@advancedenviroconsultants.com www.advancedenviroconsultants.com

Microbiological Assessment

Veteran Affairs Regional Office Building 1600 E. Woodrow Wilson Blvd.

Jackson, MS 39216

Prepared for:

Armstrong Relocation & Companies 1223 Hwy 51 North

Madison, MS 39110

Prepared by:

Advanced Environmental Consultants, Inc.

775 North President Street

Jackson, MS 39202

Project AEC 138.22

October 11, 2022 http://www.advancedenviroconsultants.com/

TABLE OF CONTENTS

Microbiological Assessment Advanced Environmental AEC 138.22 i Consultants, Inc.

Section Page

1.0 EXECUTIVE SUMMARY

2.0 SCOPE OF WORK

3.0 FIELD WORK PROCEDURES

3.1 CALIBRATION OF PUMP

3.2 SAMPLING AREAS

3.2.1 AIR MONITORING.…………………………………………………………

3.2.2 TAPE LIFTS

3.3 SUBMITTAL TO LABORATORY

3.4 MEASUREMENT OF TEMPERATURE, HUMIDITY AND RELATIVE HUMIDITY ...8

3.5 GENERAL CONDITIONS

4.0 FINDINGS

4.1 WEATHER CONDITIONS

4.2 SAMPLING METHODOLOGY

4.2.1 AIR MONITORING

4.2.2 TAPE LIFT

4.3 DISCUSSION OF FINDINGS

4.3.1 VISUAL FINDINGS

4.3.2 ODORS

4.3.3 AIR MONITORING……………………………………………………………

4.3.4 TAPE LIFT

5.0 DISCUSSION OF FUNGI

5.1 AIR MONITORING OF INTERIOR SAMPLING

5.2 TAPE LIFT

6.0 CONCLUSION

7.0 RECOMMENDATIONS

8.0 GENERAL COMMENTS

APPENDICES

APPENDIX A .................................................... LABORATORY ACCREDITATION CERTIFICATE APPENDIX B ................................................................... LABORATORY ANALYTICAL RESULTS

CHAIN OF CUSTODY

APPENDIX C ........................................................................................... SITE PHOTOGRAPHS

1.0 EXECUTIVE SUMMARY

At the request of Mr. James Gardner, Facilities Manager for the Veteran Affairs (VA) Regional Office Building, Advanced Environmental Consultants, Inc. (AEC) conducted a Microbiological Assessment of the VA Regional Office Building located at 1600 E. Woodrow Wilson Boulevard, Jackson, MS 39216. Mr. Jay Abel, Commercial Services Manager for Armstrong Relocation & Companies is the contractor who hired AEC to conduct the following Microbiological Assessment for the VA Regional Office.

AEC personnel conducted the Microbiological Assessment on Wednesday, October 5, 2022, at 8:30 a.m.

The Microbiological Assessment consisted of the following:

• A visual inspection of the affected areas building.

• A Baseline sample was collected from the exterior front entrance to the building.

• Collection of Air-o-cells at twelve (12) locations :

• Baseline – Front Entrance to the Building

• First Floor – Area Leading to the Basement

• Second Floor – Core II, Northeast corner of room

• Third Floor – Men’s Bathroom

• Third Floor – Ladies Bathroom, East

• Third Floor – Human Resources Center, Training Area

• Third Floor – Human Resources Center, Hiring Area

• Third Floor – Human Resources Center, Conference Room, Room 354

• Third Floor – Human Resources Center, Room 343, North

• Third Floor – Alice Williams’s Office, Room 302.

• Third Floor – Bobby Carter’s Office, Room 318.

• Third Floor – Stairwell

• Collection of thirteen (13) tape lifts:

• First Floor – Door at Entrance to the Basement.

• First Floor – Door Frame at Entrance to the Basement.

• First Floor – Wall leading to the Basement.

• Second Floor – Core II, Northeast Wall under the Window.

• Third Floor – Men’s Bathroom, Ceiling

• Third Floor – Ladies Bathroom, East side, Ceiling

• Third Floor – Human Resources Center, Training Area, Wall under the Window.

• Third Floor – Human Resources Center, Hiring Area, Wall under Window.

• Third Floor – Human Resources Center, Conference Room, Room 354, Wall under the Window.

• Third Floor – Human Resources Center, Room 343, North, Wall under the Window.

• Third Floor – Alice Williams’s Office, Room 302, Wall under the Window.

• Third Floor – Bobby Carter’s Office, Room 318, Wall under the Window.

• Third Floor – Southwest Quality Area, Wall under the Window.

VISUAL FINDINGS

At the time of the Microbiological Assessment, AEC personnel conducted a visual assessment of the building. The intrusion of water was observed under the outer perimeter windows on all floors (1-3), door and frame leading to the Basement, walls on stairs leading to the Basement, Second Floor (Core II, Northeast Wall under the Window, and Third Floor (Men’s Bathroom Ceiling, Ladies Bathroom Ceiling on the East side, and Stairwell).

ODORS

At the time of the Microbiological Assessment, AEC personnel did not detect any moldy odors in the building.

AIR MONITORING

First Floor - Area Leading to the Basement (AIR-02):

• There were minimal counts per cubic meter of the fungi (Cladosporium and Hyphal

Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Second Floor -Core II, Northeast Corner of Room (AIR-03):

• There were minimal counts per cubic meter of the fungi (Ascospores, Aspergillus/Penicillium-Like, Bipolaris/Dreschlera, Cladosporium, Curvularia, and Hyphal Fragments) detected in the air.

• There were moderate counts per cubic meter of the fungi (Stachybotrys) detected in the air.

• There were no elevated counts per cubic meter of fungi detected in the air.

Third Floor - Men’s Bathroom (AIR-04):

• There were minimal counts per cubic meter of the fungi (Ascospores and Hyphal

Fragments ) detected in the air.

• There were moderate counts per cubic meter of the fungi (Cladosporium) detected in the air.

• There were no elevated counts per cubic meter of fungi detected in the air.

Third Floor – Ladies Bathroom, East (AIR-05):

• There were minimal counts per cubic meter of the fungi (Ascospores, Cladosporium, and

Ganoderma) detected in the air.

• There were moderate counts per cubic meter of the fungi (Stachybotrys) detected in the air.

• There were no elevated counts per cubic meter of fungi detected in the air.

Third Floor – Human Resources Center, Training Area (AIR-06):

• There were minimal counts per cubic meter of the fungi (Ascospores, Cladosporium, Curvularia, Stachybotrys, Hyphal Fragments, and Pollen) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Third Floor – Human Resources Center, Hiring Area (AIR-07):

• There were minimal counts per cubic meter of the fungi (Cladosporium and Hyphal

Fragments ) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Third Floor – Human Resources Center, Conference Room, Room 354 (AIR-08):

• There were minimal counts per cubic meter of the fungi (Alternaria, Cladosporium, Curvularia, and Hyphal Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Third Floor – Human Resources Center, Room 343, North (AIR-09):

• There were minimal counts per cubic meter of the fungi (Ascospores, Cladosporium, Curvularia, Epicoccum, Ganoderma, and Smut/Myxomyces/Periconia) detected in the air.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Third Floor – Alice Williams’s Office, Room 302 (AIR-10):

• There were minimal counts per cubic meter of the fungi (Epicoccum, Smut/Myxomyces/Periconia, and Hyphal Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium Like) detected in the air.

Third Floor – Bobby Carter’s Office, Room 318 (AIR-11):

• There were minimal counts per cubic meter of the fungi (Ascospores, Cladosporium, Curvularia, Epicoccum, Smut/Myxomyces/Periconia, and Hyphal Fragments) detected in the air.

• There were moderate counts per cubic meter of fungi (Aspergillus/Penicillium Like) detected in the air.

• There were no elevated counts per cubic meter of fungi detected in the air.

Third Floor – Stairwell (AIR-12):

• There were minimal counts per cubic meter of the fungi (Ascospores, Basidiospores, Cladosporium, and Pollen) detected in the air.

• There were moderate counts per cubic meter of fungi (Aspergillus/Penicillium Like)

• There were no elevated counts per cubic meter of fungi detected in the air.

TAPE LIFTS

First Floor

Door at Entrance to the Basement (T-01):

• There were no minimal counts per cubic meter of fungi detected on the door.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the door.

Door Frame at Entrance to the Basement (T-13):

• There were minimal counts per cubic meter of the fungi (Ascospores and Curvularia) detected on the door frame.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and

Cladosporium) detected on the door frame.

Wall on Stairs Leading to the Basement (T-02):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

Second Floor

Core II, Northeast Wall under the Window (T-03):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the wall.

Third Floor

Men’s Bathroom, Ceiling (T-04):

• There were minimal counts per cubic meter of the fungi (Stachybotrys) detected on the ceiling.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the ceiling.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and

Cladosporium) detected on the ceiling.

Ladies Bathroom, East, Ceiling (T-05):

• There were no minimal counts per cubic meter of fungi detected on the ceiling.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the ceiling.

• There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the ceiling.

Human Resources Center, Training Area, Wall Under the Window (T-06):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

Human Resources Center Hiring Area, Wall Under the Window (T-07):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

Human Resources Center, Conference Room, Room 354, Wall Under Window (T-08):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

Human Resources Center, Room 343, North, Wall Under the Window (T-09):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and

Cladosporium) detected on the wall.

Alice Williams’s Office, Room 302, Wall Under the Window (T-10):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

Bobby Carter’s Office, Room 318, Wall Under the Window (T-11):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and

Ulocladium) detected on the wall.

Southwest Quality Area, Wall Under the Window (T-12):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the wall.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and Cladosporium) detected on the wall.

2.0 SCOPE OF WORK

The Microbiological Assessment consisted of the following:

• A visual inspection of the affected areas building.

• A Baseline sample was collected from the exterior front entrance to the building.

• Baseline – Front Entrance to the Building

• First Floor – Area Leading to the Basement

• Second Floor – Core II, Northeast corner of room

• Third Floor – Men’s Bathroom

• Third Floor – Ladies Bathroom, East

• Third Floor – Human Resources Center, Training Area

• Third Floor – Human Resources Center, Hiring Area

• Third Floor – Human Resources Center, Conference Room, Room 354

• Third Floor – Human Resources Center, Room 343, North

• Third Floor – Alice Williams’s Office, Room 302.

• Third Floor – Bobby Carter’s Office, Room 318.

• Third Floor – Stairwell

• Collection of thirteen (13) tape lifts:

• First Floor – Door at Entrance to the Basement.

• First Floor – Door Frame at Entrance to the Basement.

• First Floor – Wall leading to the Basement.

• Second Floor – Core II, Northeast Wall under the Window.

• Third Floor – Men’s Bathroom, Ceiling

• Third Floor – Ladies Bathroom, East side, Ceiling

• Third Floor – Human Resources Center, Training Area, Wall under the Window.

• Third Floor – Human Resources Center, Hiring Area, Wall under Window.

• Third Floor – Human Resources Center, Conference Room, Room 354, Wall

• under the Window.

• Third Floor – Human Resources Center, Room 343, North, Wall under the

• Window.

• Third Floor – Alice Williams’s Office, Room 302, Wall under the Window.

• Third Floor – Bobby Carter’s Office, Room 318, Wall under the Window.

• Third Floor – Southwest Quality Area, Wall under the Window.

The Air Monitoring samples identified the amount of spores that were airborne in the breathing zone.

The Tape Lift samples identified the type and quantity of fungi on the substrate. The testing is in accordance with the Environmental Protection Agency’s guidelines.

3.0 FIELD WORK PROCEDURES

3.1 Calibration of Pump

The BUCK BioAire sampling pump is used for calibration and sampling. Prior to collection of airborne spores, the pump was calibrated at a flow rate of 15 liters per minute.

3.2 Sampling Areas

Air sampling was conducted using the Buck BioAire sampling pump. The total volume for areas sampled yielded 75 liters. The sampling areas are outlined in the sections to follow.

3.2.1 Air Monitoring:

Air Monitoring was conducted in the following areas:

Baseline – Front Entrance to the Building First Floor – Area Leading to the Basement Second Floor – Core II, Northeast corner of room Third Floor – Men’s Bathroom Third Floor – Ladies Bathroom, East Third Floor – Human Resources Center, Training Area Third Floor – Human Resources Center, Hiring Area Third Floor – Human Resources Center, Conference Room, Room 354 Third Floor – Human Resources Center, Room 343, North Third Floor – Alice Williams’s Office, Room 302.

Third Floor – Bobby Carter’s Office, Room 318.

Third Floor – Stairwell

3.2.2 Tape Lifts

Tape Lifts were conducted in the following areas:

• Collection of thirteen (13) tape lifts:

First Floor – Door at Entrance to the Basement.

First Floor – Door Frame at Entrance to the Basement.

First Floor – Wall leading to the Basement.

Second Floor – Core II, Northeast Wall under the Window.

Third Floor – Men’s Bathroom, Ceiling Third Floor – Ladies Bathroom, East side, Ceiling Third Floor – Human Resources Center, Training Area, Wall under the Window.

Third Floor – Human Resources Center, Hiring Area, Wall under Window.

Third Floor – Human Resources Center, Conference Room, Room 354, Wall under the Window.

Third Floor – Human Resources Center, Room 343, North, Wall under the

Window.

Third Floor – Alice Williams’s Office, Room 302, Wall under the Window.

Third Floor – Bobby Carter’s Office, Room 318, Wall under the Window.

Third Floor – Southwest Quality Area, Wall under the Window.

3.3 Submittal to Laboratory

AEML was the laboratory used for this project. AEML is accredited with the American Industrial Hygiene Association. A copy of the laboratory certification is included in Appendix A.

The samples were packed and shipped via federal express air to AEML for analysis. The laboratory data and chain of custody is included in Appendix B.

3.4 Measurement of Temperature, Humidity and Relative Humidity

The outdoor temperature, humidity, and environmental pollen count were recorded for the sampling event on October 5, 2022, utilizing the weather channel website with Jackson, MS zip code (39216) as reference.

3.5 General Conditions

AEC personnel conducted air monitoring in the building.

At the time of the Microbiological Assessment, AEC personnel conducted a visual assessment of the building. The intrusion of water was observed under the outer perimeter windows on all floors (1-3), door and frame leading to the basement, wall on stairs leading to the basement, Second Floor (Core II, Northeast Wall under the Window), and Third Floor (Men’s Bathroom Ceiling, Ladies Bathroom Ceiling on the East side, and Stairwell).

4.0 FINDINGS

Laboratory Data and Chain of Custody are enclosed in Appendix B. Photographs of areas sampled are provided in Appendix C. During the sampling event, weather conditions are outlined on the following page.

4.1 Weather Conditions

Sampling

Date Outdoor Temperature

(oF) Humidity (percent)

Pollen Counts Indoor Relative Humidity (percent)

10/5/22 60° 51% Medium High (8.0) N/A

4.2 Sampling Methodology

4.2.1 Air Monitoring

Sampling was conducted by drawing a measured volume (15 liters) of air through the sampling pump for a specific length of time (5 minutes) yielding a total of 75 liters. The collection of this volume was trapped in the sampling media (biocassette/air-o-cell), which was analyzed by direct microscopic examination by AEML. The air-sampling event was accompanied by an outside sample, which is used as a reference or comparison tool. Since mold / fungi spores are ubiquitous and always present in breathing zone level, a comparison guide is necessary to determine the representative total spores counts and types present in the indoor environment to outdoor environment.

4.2.2 Tape Lift

Sampling was conducted by placing tape on the area where mold was visible. A smooth surface was pressed over the tape, removed, and transferred to a zip lock bag.

The tape was analyzed by direct microscopic examination by AEML, Inc. Microbiology Laboratories.

4.3 Discussion of Findings

4.3.1 Visual Findings

At the time of the Microbiological Assessment, AEC personnel conducted a visual assessment of the building. The intrusion of water was observed under the outer perimeter windows on all floors (1-3), door and frame leading to the Basement, wall on stairs leading to the Basement, Second Floor (Core II, Northeast Wall under the Window), and Third Floor (Men’s Bathroom Ceiling, Ladies Bathroom Ceiling on the east side, and Stairwell).

4.3.2 Odors

At the time of the Microbiological Assessment, AEC personnel did not detect a moldy odor in the building.

4.3.3 Air Monitoring

First Floor - Area Leading to the Basement (AIR-02):

• There were minimal counts per cubic meter of the fungi (Cladosporium Hyphal

Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium

Like) detected in the air.

Second Floor -Core II, Northeast Corner of Room (AIR-03):

• There were minimal counts per cubic meter of the fungi (Ascospores, Aspergillus/Penicillium-Like, Bipolaris/Dreschlera, Cladosporium, Curvularia, and Hyphal Fragments) detected in the air.

• There were moderate counts per cubic meter of the fungi (Stachybotrys) detected in the air.

Third Floor - Men’s Bathroom (AIR-04):

• There were minimal counts per cubic meter of the fungi (Ascospores and Hyphal

Fragments ) detected in the air.

• There were moderate counts per cubic meter of the fungi (Cladosporium) detected

Third Floor – Ladies Bathroom, East (AIR-05):

• There were minimal counts per cubic meter of the fungi (Ascospores, Cladosporium, and Ganoderma) detected in the air.

• There were moderate counts per cubic meter of the fungi (Stachybotrys) detected

Third Floor – Human Resources Center, Training Area (AIR-06):

Cladosporium, Curvularia, Stachybotrys, Hyphal Fragments, and Pollen) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

Third Floor – Human Resources Center, Hiring Area (AIR-07):

• There were minimal counts per cubic meter of the fungi (Cladosporium and Hyphal

Fragments ) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

Third Floor – Human Resources Center, Conference Room, Room 354 (AIR-08):

• There were minimal counts per cubic meter of the fungi (Alternaria, Cladosporium, Curvularia, and Hyphal Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

Third Floor – Human Resources Center, Room 343, North (AIR-09):

Cladosporium, Curvularia, Epicoccum, Ganoderma, and Smut/Myxomyces/Periconia) detected in the air.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected in the air.

Third Floor – Alice Williams’s Office, Room 302 (AIR-10):

• There were minimal counts per cubic meter of the fungi (Epicoccum, Smut/Myxomyces/Periconia, and Hyphal Fragments) detected in the air.

• There were no moderate counts per cubic meter of fungi detected in the air.

Third Floor – Bobby Carter’s Office, Room 318 (AIR-11):

Cladosporium, Curvularia, Epicoccum, Smut/Myxomyces/Periconia, and Hyphal Fragments) detected in the air.

• There were moderate counts per cubic meter of fungi (Aspergillus/Penicillium Like)

Third Floor – Stairwell (AIR-12):

Basidiospores, Cladosporium, and Pollen) detected in the air.

• There were moderate counts per cubic meter of fungi (Aspergillus/Penicillium Like)

4.3.4 Tape Lift

First Floor

Door at Entrance to the Basement (T-01):

• There were no minimal counts per cubic meter of fungi detected on the door.

• There w ere moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the door.

Door Frame at Entrance to the Basement (T-13):

• There were minimal counts per cubic meter of the fungi (Ascospores and

Curvularia) detected on the door frame.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like and Cladosporium) detected on the door frame.

Wall on Stairs Leading to the Basement (T-02):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the wall.

Second Floor

Core II, Northeast Wall under the Window (T-03):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the wall.

Third Floor

Men’s Bathroom, Ceiling (T-04):

• There were minimal counts per cubic meter of the fungi (Stachybotrys) detected on the ceiling.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium- Like and Cladosporium) detected on the ceiling.

Ladies Bathroom, East, Ceiling (T-05):

• There were no minimal counts per cubic meter of fungi detected on the ceiling.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the ceiling.

Human Resources Center, Training Area, Wall Under the Window (T-06):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the wall.

Human Resources Center, Hiring Area, Wall Under the Window (T-07):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the wall.

Human Resources Center, Conference Room, Room 354, Wall Under Window (T-08):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the wall.

Human Resources Center, Room 343, North, Wall Under the Window (T-09):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like and Cladosporium) detected on the wall.

Alice Williams’s Office, Room 302, Wall Under the Window (T-10):

• There were no minimal counts per cubic meter of fungi detected on the wall.

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

• There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-

Like) detected on the wall.

Bobby Carter’s Office, Room 318, Wall Under the Window (T-11):

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

Like and Ulocladium) detected on the wall.

Southwest Quality Area, Wall Under the Window (T-12):

• There were moderate counts per cubic meter of the fungi (Hyphal Fragments) detected on the door frame.

Like and Cladosporium) detected on the wall.

5.0 DISCUSSION OF FUNGI

5.1 Air Monitoring of Interior Sampling

• Alternaria (all-tur-nair’-ee-uh) – common allergen / contaminant / opportunistic pathogen, one of the most common molds found world wide in soil and on plants and can commonly can be found indoors (frequently appearing black on window frames). It is an important airborne allergen and common agent for hay fever, asthma, and other allergy related symptoms.

• Ascospores (ass-co’-spores) - A large category of spores (produced in a sac-like structure) that are found everywhere in nature and include more than 3000 genera.

Most Ascospores of health or IAQ importance are identified separately by their genus (e.g. Chaetomium) when possible on a IAQ report, and the Ascospore category is used primarily on these reports for a large group of less important spore types often found in quantity on outdoor air samples. On tape samples, Ascospore is sometimes also used as a general morphological identification (i.e. the ascus or sac structure is present) for certain samples in those cases when the spores do not appear to represent any of the IAQ significant genera.

• Aspergillus (as-per-jill-us) – allergen / contaminant / opportunistic pathogen, commonly found in the environment around the world. It comprises approximately 200 species and can appear almost any color. Though commonly found on cultures, tape-lifts, and air samples, its spores are indistinguishable from Pencillium on non-cultured samples (like tape-lifts and air-o-cells) unless the conidiophore is present.

Health effects vary by species, but many species are reported to be allergenic.

Some species produce toxins that might have significant health effects in humans.

Aspergillus is one of the most infectious of molds, but infections are not common in normal immune systems. In immuno-compromised individuals, however, the disease Aspergillosis is a very significant and potentially deadly health concern.

• Basidiospores (bah-sid-ee-oh’-spores) - allergen / contaminant, a general class of spore formed on a structure known as a basidium, characteristic of the Basidiomycete class (that includes rusts, smuts and mushrooms). This category is commonly found in outdoor air samples. Many species are reported to be allergenic and some species are associated with dry rot in wood. Elevated airborne concentrations indoors might be indicative of water damage or too high of humidity.

• Bipolaris/Dreschlera – Toxigenic. Also recognized as an allergen. Under certain conditions, this fungi has been documented to produce the mycotoxin, sterigmatocystin. Studies have indicated that this toxin may cause damage to the liver and kidneys in laboratory animals.

• Cladosporium species (clad-oh-spore-ee-um) – common allergen / contaminant / very rarely pathogenic, found everywhere, many times the most common and numerous mold found in outdoor air. Indoor concentrations are usually not as high, but it is an important airborne allergen and common agent for hay fever, asthma, and other allergy related symptoms. It can thrive in various indoor environments, appearing light green to black (the black mold on air vent grills is usually Cladosporium).

• Curvularia (curve-you-lair’-ee-uh) - contaminant / opportunistic pathogen, found in air, soil and textiles. Reported to be allergenic. Rare infections of corneas, nails, and sinuses, primarily in immunocompromised individuals. Curvularia species- are allergenic molds that grow on cellulose surfaces. These species are often found growing on a variety of building materials. Some people may experience hay fever, asthma and or allergic fungal sinusitis.

• Epicoccum (epp-ee-cock’-um) - contaminant / opportunistic pathogen, found in soil, air, water and rotting vegetation and can be commonly found in outdoor air. It is a common allergen, and rarely it can cause an infection in the skin.

• Ganoderma - are wood-decaying fungi with a cosmopolitan distribution. They can grow on both coniferous and hardwood species. They are white-rot fungi with enzymes that allow them to break down wood components, such as lignin and cellulose.

• Hyphal Fragments- Hyphal fragments or mycelia are components of fungal growth (similar to the roots and branches of a tree); it is common to find small hyphal fragments in outdoor air and possibly in indoor dust. However, their presence in indoor air samples, if in quantity or in large segments, suggests an active fungal colony in the building. Their presence in a surface sample in quantity or in large segments indicates that active fungal growth is present or nearby, or that fungal material has been disturbed in the building. May be allergenic.

• Smuts/Myxomyces/Periconia - general category for commonly found genera usually associated with living and decaying plants as well as decaying wood. Sometimes can be found indoors. Some allergenic properties reported, but generally pose no health concerns to humans or animals.

• Stachybotrys (stack-ee-bought-ris) - contaminant, found indoors primarily on wet cellulose containing materials. It is the "toxic black mold" that has garnered much media attention. Some species produce a potent toxin that is lethal to animals, though dose effect on humans is not clear. One species produces a toxin linked to the bleeding lung deaths of several infants. A host of other toxic reactions in humans are also linked to it, but many of these require further study. Stachybotrys is sometimes difficult to detect indoors because many times it will grow unseen on the back of walls or in the wall cavity with little disturbance that would cause it to be detected by routine air sampling. This is potentially also when it is of most health concern: when it covers entire wall areas and constantly produces toxins undetected. Non-cultured lab analyses (air-o-cells and tape-lifts) usually are the proper method of identification because Stachybotrys does not grow or compete well on most culture plate media, and it is reported that even non-viable spores can be toxigenic.

• Ulocladium - (you-low-clay-dee-um) - contaminant, found everywhere. Can grow indoors on various materials including paper, but requires more water than some other molds. It is reported to be a major allergen. Common outdoors, grows on decaying plants and organic matter.

5.2 Tape Lift

• Ascospores (ass-co’-spores) - A large category of spores (produced in a sac-like structure) that are found everywhere in nature and include more than 3000 genera.

Most Ascospores of health or IAQ importance are identified separately by their genus (e.g. Chaetomium) when possible on a IAQ report, and the Ascospore category is used primarily on these reports for a large group of less important spore types often found in quantity on outdoor air samples. On tape samples, Ascospore is sometimes also used as a general morphological identification (i.e. the ascus or sac structure is present) for certain samples in those cases when the spores do not appear to represent any of the IAQ significant genera.

• Aspergillus (as-per-jill-us) – allergen / contaminant / opportunistic pathogen, commonly found in the environment around the world. It comprises approximately 200 species and can appear almost any color. Though commonly found on cultures, tape-lifts, and air samples, its spores are indistinguishable from Pencillium on non-cultured samples (like tape-lifts and air-o-cells) unless the conidiophore is present.

Health effects vary by species, but many species are reported to be allergenic.

Some species produce toxins that might have significant health effects in humans.

Aspergillus is one of the most infectious of molds, but infections are not common in normal immune systems. In immuno-compromised individuals, however, the disease Aspergillosis is a very significant and potentially deadly health concern.

• Cladosporium species (clad-oh-spore-ee-um) – common allergen / contaminant / very rarely pathogenic, found everywhere, many times the most common and numerous mold found in outdoor air. Indoor concentrations are usually not as high, but it is an important airborne allergen and common agent for hay fever, asthma, and other allergy related symptoms. It can thrive in various indoor environments, appearing light green to black (the black mold on air vent grills is usually Cladosporium).

• Stachybotrys (stack-ee-bought-ris) - contaminant, found indoors primarily on wet cellulose containing materials. It is the "toxic black mold" that has garnered much media attention. Some species produce a potent toxin that is lethal to animals, though dose effect on humans is not clear. One species produces a toxin linked to the bleeding lung deaths of several infants. A host of other toxic reactions in humans are also linked to it, but many of these require further study. Stachybotrys is sometimes difficult to detect indoors because many times it will grow unseen on the back of walls or in the wall cavity with little disturbance that would cause it to be detected by routine air sampling. This is potentially also when it is of most health concern: when it covers entire wall areas and constantly produces toxins undetected. Non-cultured lab analyses (air-o-cells and tape-lifts) usually are the proper method of identification because Stachybotrys does not grow or compete well on most culture plate media, and it is reported that even non-viable spores can be toxigenic.

6.0 CONCLUSION

The findings from the Microbiological Assessment concluded the following in those areas tested.

• Visual Signs of Water Intrusion: The intrusion of water was observed under and around the outer perimeter windows of the first, second, and third floors, the wooden door and metal door frame leading to the Basement, wall of Stairs leading to the Basement, Second Floor Core II, Northeast Wall under the Window, and the Third Floor Men’s Bathroom Ceiling, Ladies Bathroom Ceiling on the East side, and Stairwell. Mold was visually observed on the walls under the windows in all areas tested, the wooden door and metal door frame leading to the Basement, the wall of the stairs leading to the Basement as well as the ceiling in the Third Floor Men’s Bathroom and the Third Floor Ladies Bathroom on the east side of the building.

• Odor: At the time of the Microbiological Assessment, AEC personnel did not detect any moldy odors in those areas assessed.

• Air Monitoring: There were twelve air-o-cells collected at designated locations where mold existed. The findings depicted below identify those areas with elevated levels on fungi:

o First Floor – Area Leading to the Basement – There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

o Third Floor – Human Resources Center, Training Area - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

o Third Floor – Human Resources Center, Hiring Area - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

o Third Floor – Human Resources Center, Conference Room, Room 354 - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

o Third Floor – Human Resources Center, Room 343, North - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

o Third Floor – Alice Williams’ Office, Room 302 - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected in the air.

• Tape Lifts: There were thirteen tape lifts collected at designated locations where mold existed. The findings depicted below identify those areas with elevated levels on fungi:

o First Floor: Door at Entrance to Basement – There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the door.

o First Floor: Door Frame at Entrance to Basement - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the door frame.

o First Floor: Wall of Stairs Leading to the Basement - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

o Second Floor: Core II, Northeast Wall under the Window – There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the wall.

o Third Floor: Men’s Bathroom, Ceiling - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and Cladosporium) detected on the ceiling.

o Third Floor: Ladies Bathroom, Ceiling, East - There were elevated counts per cubic meter of the fungi (Stachybotrys) detected on the ceiling.

o Third Floor: Human Resources Center, Training Area, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

o Third Floor: Human Resources Center, Hiring Area, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

o Third Floor: Human Resources Center, Conference Room, Room 354, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

o Third Floor: Human Resources Center, Room 343, North, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium- Like and Cladosporium) detected on the wall.

o Third Floor: Alice Williams’ Office, Room 302, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like) detected on the wall.

o Third Floor: Bobby Carter’s Office, Room 318, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and Ulocladium) detected on the wall.

o Third Floor: Southwest Quality Area, Wall Under Window - There were elevated counts per cubic meter of the fungi (Aspergillus/Penicillium-Like and Cladosporium) detected on the wall.

Based on the findings of this assessment on October 5, 2022, AEC concludes the following:

The fungi levels detected in the laboratory readings are extremely elevated and well above the baseline sample’s readings in both air and tape lift sampled areas. The baseline sample that was taken at the time of testing, was used as a control to gauge what fungi levels may be normal for exterior sampling in this particular area. We use the baseline sample to compare to our interior sample data to measure what is naturally occurring on the exterior of the building.

Elevated levels of Aspergillus/Penicillium-Like and Cladosporium fungi, although classified as allergenic molds has the potential to become toxic in extreme amounts. Allergenic molds may cause allergic or asthmatic symptoms, but generally these types of fungi can be abated safely.

The levels of Aspergillus/Penicillium-Like fungi detected in the samples may result in an indoor reservoir for water existing. Because Aspergillus/Penicillium-Like and Cladosporium fungi are water markers, it is evident that water intrusion is present in the areas where these two organisms are present.

Hyphal Fragments are single, unidentifiable fragments of mold. Hyphal Fragments were detected in nine of the twelve air samples obtained in minimal to moderate levels and in all tape lift samples in moderate levels. High amounts of hyphal fragments are typically anything above 100 count/m3. The Hyphal Fragments that were detected in the nine of the twelve air samples where it surfaced ranged from 27 count/m3 to 2,280 count/m3, with 53 count/m3 being detected in our control (Baseline sample). Hyphal Fragments typically indicate that active fungal growth is nearby.

Stachybotrys fungi is classified as a toxic fungi. Stachybotrys was found in both elevated, moderate and minimal quantities. Despite being found in minimal quantities in select areas of the building, there is always the potential to increase in toxicity when there is a continuous flow of water. Stachybotrys species need a direct water source to grow. Trace levels (0 - 50 spore count) of spores are not an issue. Toxic mold species like Stachybotrys, however; are an issue at a lower spore count level. Stachybotrys in conjunction with water markers like Chaetomium or high levels of Aspergillus/Penicillium are considered an issue in low levels.

There are no national standards for the number of fungal spores that may be present in the indoor environment. As a general rule and guideline that is widely accepted in the indoor air quality field, the numbers and types of spores that are present in the indoor environment should not exceed those that are present outdoors at any given time. There will always be some mold spores present in “normal” environments. The purpose of sampling and counting spores is to help determine whether an abnormal condition exists within the indoor environment and if it does, to help pinpoint the area of contamination. Spore counts should not be used as the sole determining factor of mold contamination. Many factors can cause anomalies in the comparison of indoor and outdoor samples due to the dynamic nature of both environments.

7.0 RECOMMENDATIONS

AEC recommends where the intrusion of water is introduced, water must be stopped. Fungi usually exists where there is or has been a possible leak. Water intrusion is a viable source to encourage mold growth. The client is advised that Microbiological growth will occur if the source of moisture / water is not remedied.

The intrusion of water has entered the building around the outer perimeter windows on all floors.

In those areas where elevated levels of fungi existed, the areas should be sealed off, intrusion of water stopped, and fungi remediated. Additionally, all wallpaper, drywall, and carpet should be removed along with any furnishings in those areas that came in contact with water or extreme moisture. After remediation, a Microbiological Clearance should be conducted to assure that the fungi count has been reduced and the areas tested are at safe levels for occupancy.

The Environmental Protection Agency (EPA) recommends that any indoor mold growth be addressed and all water or moisture sources be eliminated. For the EPA’s publication, “A Brief Guide to Mold, Moisture, and Your Home” visit the website http://www.epa.gov/iaq/molds/images/moldguide.pdf.

Currently, there are no regulations on mold practices provided by the State of Mississippi Department of Environmental Quality (MDEQ) or United States Environmental Protection Agency (EPA). The recommendations are based on the accepted industry standards developed by the American Conference of Governmental Industrial Hygiene (ACGIH), American Industrial Hygiene Association (AIHA), Indoor Air Quality Association (IAQA), and Centers for Disease Control (CDC). This air monitoring sampling event was conducted in accordance with the Environmental Protection Agency (EPA) guidelines.

http://www.epa.gov/iaq/molds/images/moldguide.pdf

8.0 GENERAL COMMENTS

This report is an instrument of service prepared for the exclusive use of the Veteran Affairs Regional Office Building, and may not be reproduced or distributed without written authorization from said party. The services described in this report were performed consistent with generally accepted professional consulting principles and practices for microbiological constituents only. No other warranty, expressed or implied, is made. The services were performed consistent with the agreement between AEC and the Armstrong Relocation & Companies. This report is solely for the use and information of the Veteran Affairs Regional Office Building or as otherwise noted. Any unauthorized use of this report is strictly prohibited and AEC assumes no liability for any such use.

This report has been prepared in order to aid in the evaluation of the Veteran Affairs Regional Office Building, with regard to mold at the time of this study. The conclusions presented in this report were based on information obtained during this assessment. We do not warrant the accuracy of information supplied by others.

Microbiological Assessment Advanced Environmental AEC 138.22 Consultants, Inc.

APPENDICES

APPENDIX A

LABORATORY ACCREDITATION CERTIFICATE

(A2LA Cert. No. 2572.01) 05/25/2021 Page 1 of 1

SCOPE OF ACCREDITATION TO ISO/IEC 17025:2017

AEML, INC.

601 East Atlantic Blvd.

Pompano Beach, FL 33060 Ronald Mazur Phone: (954) 333-8149

BIOLOGICAL

Valid To: July 31, 2023 Certificate Number: 2572.01

In recognition of the successful completion of the A2LA evaluation process (including an assessment of the laboratory's compliance with ISO IEC 17025:2017 and the Texas DOH Indoor Air Quality Testing Laboratory Accreditation Program), accreditation is granted to this laboratory to perform the following types of biological and microbiological tests on: air, bulk, surface, water, Air-o-Cell, Micro-5, Mold-Snap, Via Cell, VersaTrap, Cyclex-D, Buck BioAire, and similar sampling systems:

SOP #

Test Code(s)

Title

Microscopy

AEML Doc 18 A001, A002, A003 Microscopic Spore Trap Analysis by

Direct Examination

AEML Doc 19

T001, T002 Microscopic Screen Analysis by Direct Examination – Tape/Bulk Samples

S001, S002 Microscopic Screen Analysis by Direct Examination – Swab Samples

For the tests to which this accreditation applies, please refer to the laboratory’s Biological Scope of Accreditation.

Accredited Laboratory

A2LA has accredited

AEML, INC.

Pompano Beach, FL for technical competence in the field of

Biological Testing

This laboratory is accredited in accordance with the recognized International Standard ISO/IEC 17025:2017 General requirements for the competence of testing and calibration laboratories. This laboratory also meets the requirements of A2LA R215 - Specific Requirements- Texas DOH Indoor Air Quality Testing Laboratory Accreditation Program. This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management system (refer to joint ISO-ILAC-IAF Communiqué dated April 2017).

Presented this 25th day of May 2021.

Vice President, Accreditation Services For the Accreditation Council Certificate Number 2572.01 Valid to July 31, 2023

APPENDIX B

LABORATORY ANALYTICAL RESULTS

CHAIN OF CUSTODY

AEML, Inc.

601 E. Atlantic Blvd.

Pompano Beach, FL 33060 Batch:

Phone: (954) 333-8149

AEML Test: A001 Spore Trap Analysis email: customerservice@aemlinc.com

% 1 1 K

% 1 1 K

12 ―

4 100

46 ―

26 <1

1 ―

1 ―

3 ―

1 ―

5 ―

1 ―

* Debris rating due to spores.

# Estimation performed due to high count.

1,541 20,547

2 27

2 27

1,539 # 20,520

153 2,040

4 53

1 13

8 107

1 13

1 13

5 67

40 533

2 27

Debris Rating

Detection Limit

19 253

6 80

70 933

Ulo…