QIAGEN EZ2 Connect Fx Specifications.pdf

January 2025

Sole Source Specification QIAGEN® EZ2® Connect Fx

9003220

QIAGEN, GmbH, QIAGEN Strasse 1, 40724 Hilden, GERMANY

Sample to Insight

2 EZ2 Connect Fx Sole Source Specification | 01/2025

QIAGEN EZ2 Connect Fx

Sole Source Specification

The QIAGEN EZ2 Connect Fx (cat. no. 9003220) is designed for automated isolation of nucleic acids from a wide variety of sample types. The EZ2 Connect Fx with MagAttract® silica magnetic particle technology combines the speed, efficiency, and purity of silica-based nucleic acid purification, with the convenient handling of magnetic particles. Plus, the

EZ2 Connect Fx provides full walk-away automation; proven self-contained, sealed reagent strip chemistries; and gDNA, mitoDNA, or RNA extraction protocols for multiple sample types.

We hereby certify that QIAGEN LLC and its corporate affiliate, QIAGEN GmbH, are the only vendors that can meet the specifications below. The QIAGEN EZ2 Connect Fx (cat. no.

9003220) and related products (EZ1&2™ DNA Investigator Kits) are solely distributed by

QIAGEN subsidiaries and official QIAGEN distributors in the United States and Canada.

Sole source specifications/customer requirements

The EZ2 Connect Fx meets all of the following criteria and specifications:

l Vendor instrument (EZ2 Connect Fx) offers the ability to run up to 24 samples at a time.

l Vendor instrument (EZ2 Connect Fx) offers extraction that takes place within a filtered tip, minimizing or eliminating cross contamination.

l Vendor instrument (EZ2 Connect Fx) offers protocols using “tip-dance”, which enables the lab to keep sample substrate in the sample tube during extraction, eliminating an extra transfer and/or centrifugation step.

l Vendor instrument (EZ2 Connect Fx) offers an integrated UV LED to decontaminate the robotic work deck.

EZ2 Connect Fx Sole Source Specification | 01/2025 3 l Vendor instrument (EZ2 Connect Fx) offers the ability to track run data, including reagent and sample data. Individual components include:

o Vendor instrument (EZ2 Connect Fx) offers sample tracking capabilities throughout the purification via a barcode reader connected to the instrument. Data collected includes sample and reagent information.

o Vendor instrument (EZ2 Connect Fx) offers the ability to provide instrument alerts or status updates to a remote PC, smart phone, or tablet.

o Vendor instrument (EZ2 Connect Fx) allows the transfer of data via a USB port.

o Vendor instrument (EZ2 Connect Fx) data reporting is compatible with commonly used

Laboratory Information Management Systems (LIMS).

l Vendor instrument (EZ2 Connect Fx) offers the capability of an automated “load-check” function that ensures correct loading of plasticware and reagents prior to extraction run commencement.

l Vendor instrument (EZ2 Connect Fx) should offer protocols and required hardware (“Flip

Cap Rack”) that enable the lab to elute the DNA directly into the final 1.5 mL, screw-cap storage tube, eliminating an extra transfer step by the end user.

l Vendor kit (EZ1&2 DNA Investigator Kit) should offer a single kit, the EZ1&2 DNA

Investigator Kit (48), which contains sealed reagent strips suitable for performing DNA extractions for both CODIS/NDIS DNA and trace DNA casework.

l Vendor instrument/kit (EZ2 Connect Fx/EZ1&2 DNA Investigator Kit) should allow the end user to select to elute the DNA with either water or low TE buffer, both of which are contained in the reagent strip. Elution volume should be selectable from 20 to 200 µL.

l Vendor instrument (EZ2 Connect Fx) should offer existing protocols for casework and reference samples for each sample type used in the DNA forensics lab including liquid blood, dried blood spots, buccal swabs, saliva on cards, hair, tissue, fingernails, feces, 4 EZ2 Connect Fx Sole Source Specification | 01/2025 clothing, cigarette butts, differential extractions (epithelial and sperm cells), mouth washes, and DNA clean-up of organic extractions.

l Vendor instrument (EZ2 Connect Fx) should be able to process samples using the EZ1&2

DNA Investigator Kit in less than 20 minutes.

l Vendor instrument (EZ2 Connect Fx) offers a sample recovery protocol. In the event of an instrument crash/error, the recovery protocol function guides the user to recover the affected sample.

Unique application specifications l Reliable, high-quality nucleic acid suitable for qPCR and STR downstream applications from cultured cells, blood, blood on clothes, dried blood spots, hair, nail clippings, swabs from handled materials (guns, bats, knives, etc.), sperm cells, buffy coat, buccal cells, mouth washes, paraffin-embedded sections, FFPE samples, urine, cerebral spinal fluid, soil, air filters, water, bone, stool, cigarette butts, nasal washes, and nasal swabs. The

EZ2 Connect Fx offers specific protocols for viral nucleic acid, genomic DNA, mitoDNA, total RNA, total nucleic acid and bacterial DNA from multiple sample types.

o Standardization of sample preparation eliminating variability between technicians.

o Efficient sample processing – fully automated for true walk-away capability. Clear on-screen messages tell you exactly what step the instrument is processing and the time to completion.

l The proprietary and unique self-contained, sealed Reagent Cartridge Strips offer:

o Safety – no contact or dispensing of reagents.

o Ease of use – reagent strips are easily loaded into the instrument, without prior opening.

o Time savings – loading of reagent strips and samples takes less than 5 minutes.

EZ2 Connect Fx Sole Source Specification | 01/2025 5 l MagAttract chemistry is offered on higher-throughput QIAGEN robotic systems, allowing for scale up in the future without a need to change chemistry.

l Total sample extraction time is less than 20 minutes.

Power 100–120/200–240V AC ± 10%, 50/60 Hz, 1000VA

Operating temperature 18–30°C (64–86°F)

Operating humidity 10–75% RH

Altitude Up to 2000 m (6500 ft)

Place of operation For indoor use only

Storage temperature 5–40°C (41–104°F) in manufacturer's package

Pipettor head Contains 24 high-precision syringe pumps, each containing a tip adapter that attaches to fil-ter-tips. Each tip aspirates and dispenses 20–1000 μL liquid.

Magnet system M-axis contains a magnet that can be moved in Z-direction above the worktable to attract particles within tips attached to the pipettor head.

Pipetting accuracy 20–49 μL: ± 15%

50–99 μL: ± 10%

100–1000 μL: ± 8%

Syringe pumps are air-filled.

Liquids containing salts, alcohol, solvents and/or magnetic particles can be aspirated and dispensed. Air gaps can be aspirated to prevent aspirated liquid from dripping.

Filter tips Filter-tips are picked up from the tip holder and ejected back into the tip holder. The EZ2 Connect Fx accommodates up to 48 tip holders, each containing a filter-tip, in the tip rack on the worktable.

Heating system Accommodates the heating positions of reagent cartridges and has a temperature range of between ambient temperature and 95ºC (203ºF).

Heating block accuracy ±2°C*

UV-LED Wavelength: 270−285 nm

Barcode reader Decoding capability: 1D, 2D, postal codes and stocked codes

Reading range (depth of field) for Code 39 barcode (5 mils): 7.0 to 38.0cm

Operating Temperature: 0−50°C

Storage Temperature: −40°C to 70°C

Light Green/Light Grey Reading angle (pitch): ±65 degrees

Resolution (maximum): 0.102mm/4 mils (1D Linear) Interface: USB

Internal camera OPTICS: Standard Density (5.2 mm), High-Density (8.0 mm), UHD (16.0 mm)

FOCUS: SD, HD: Factory Set to 50, 102, 190, Display Screen Size 10.1 (diagonal) inch

Display Format 1280(H) x (R,G,B) x 800(V) dots

LCD Active Area 216.96(H) ×135.6 (V) mm

Sensor active area 218.96(H) ×137.6(V) mm

Pixel Pitch 0.1695(H) × 0.1695(V) mm

Pixel Configuration RGB-Stripe

Outline Dimension 295(H) ×191.95(V) ×13.86Max(D) mm

Back-light LED LCM Interface LVDS View direction ALL

Pollution level 2

Environmental class Operation: 3K21 (IEC 60721-3-3), 3M11 (IEC 60721-3-3)

Storage: 1K21 (IEC 60721-3-1), 1M11 (IEC 60721-3-1)

Transport: 2K11 (IEC 60721-3-2), 2M4 (IEC 60721-3-2)

Data output USB port: USB2.0; Compatible OS: Windows 10; Mac OS x 10,1 or later, File system format: FAT32

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.

Trademarks: QIAGEN®, Sample to Insight®, EZ2®, EZ1&2™, MagAttract® (QIAGEN Group. Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

01/2025 PROM-18044-005 © 2025 QIAGEN, all rights reserved.

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