PERFORMANCE WORK STATEMENT (PWS) &

TECHNICAL SPECIFICATION

DNA Sequencing Equipment & Consumables Package

1.0 Introduction/Background

This Performance Work Statement (PWS) outlines the detailed requirements for a complete and integrated ensemble of laboratory test equipment, ancillary hardware, and chemical consumables. The ensemble is intended for conducting comprehensive DNA marking detection, QC authentication, PCR amplification, and capillary electrophoresis.

2.0 Scope

The contractor shall provide a turnkey laboratory equipment ensemble. The configuration must match or exceed the capabilities of the 3500 Genetic Analyzer 8 Channel (Model No: 622-0010) and the Applied Biosystems 2720 Thermal Cycler (Model No: 4359659R), paired with GeneMapper 6 software on a Windows 11 workstation. Additionally, a comprehensive starter kit of PCR reagents and swabbing tools is required. The contractor shall also provide 1 day of on-site training for up to 6 individuals upon installation.

3.0 Operational & Process Requirements

The equipment and consumables must support the following laboratory workflows:

• Sample Swabbing & Initial Detection: Utilization of UV Flashlights and IR Lasers to detect rapid reporters.

Swabbing of substrates (Nickel, Gold, Epoxy, Alumina) using Synthetic Nylon Swabs, Puritan Cotton Swabs, cut via razors into 0.2 mL Flat cap PCR reaction tubes.

• PCR Master Mix Preparation: Must be performed in a dedicated DNA Free Hood to prevent contamination.

• DNA Amplification: Utilizing the Thermal Cycler to perform precise heating and cooling cycles, incorporating fluorescent tags to the target DNA.

• Capillary Electrophoresis: Utilizing the 3500 Genetic Analyzer to separate DNA based on size, with software interpretation of specific 3-clone DNA combinations.

4.0 Technical Specifications

The following specific equipment, software, services, and consumables, OR EQUIVALENTS are required:

Equipment & Services Requirements

Component / Part Specification / Requirement

Sequencing Platform 3500 Genetic Analyzer 8 Channel (Model No: 622-0010) OR EQUIVALENT.

Thermal Cycler Applied Biosystems 2720 Thermal Cycler (Model No: 4359659R) OR EQUIVALENT.

Workstation & Software Windows 11 PC running GeneMapper 6 OR EQUIVALENT.

Ancillary Lab Hardware DNA Free Hood, Plate Centrifuge, UV Flashlight, IR Laser, Mini desktop Centrifuge, Vortex Mixer.

4405778 3500 SEQ FRAG INSTALL KIT (Applied Biosystems Sequencing and Fragment Installation Kit for 3500 Series Genetic Analyzer)

4428259 SVC IQOQIPV 3500 SEQ/FRAG

4404685 CAPILLARY ARRAY 8-CAP 50CM RUO (Format: 8-Capillary Array, Capillary Length: 50 cm)

N8010560 MICROAMP 96-WELL RXN PLATE - Applied Biosystems MicroAmp Optical 96-Well Reaction Plate (10 Plates)

NC2164454 ON-SITE TRAINING

4393927 ANODE BFFR CONTAINR 3500SERIES (Contains 4 packs of anode buffer container)

4408256 CATHODE BFR CONTAINR 3500 SER (Contains 4 packs of cathode buffer container)

4393718 CONDITIONING REAGNT 3500SERIES (Applied Biosystems Conditioning Reagent, for 3500/SeqStudio Flex)

4408399 GENESCAN-600 LIZ SIZE STD V2.0 (Kit contains enough standard for 800 analyses)

4311320 HI-DI FORMAMIDE BOTTLE 25 ML (Applied Biosystems Hi-Di Formamide, Liquid)

4393708 POP-7 384 POLYMER 3500 SERIES

ZG11SC3500 AB ASSURANCE 3500

Required Consumables (PCR Master Mix & Prep)

Reagent / Item Vendor / Specification Part / Catalog Number

LyoReady Mix Meridian Bioscience MDX021

10uM Primers IDT (Forward and Reverse "NCFR") N/A (Sequence specific)

5M Betaine Sigma Aldrich B0300

DMSO Sigma Aldrich D8418

PEG 400 Sigma Aldrich 81172

Mg+ Solution New England Biolabs B9021S

10% BSA Sigma Aldrich 126609

PCR Certified Water Teknova W3330

Swabbing Tools Synthetic Nylon Swabs, Puritan Cotton Swabs, Tongue Depressors, Razors

Standard Commercial

Solvents Ethanol and/or 2-Butanone (MEK) Standard Commercial

Lab Tubes 0.2 mL Flat cap PCR tubes, 2 mL microcentrifuge tubes Standard Commercial

5.0 Installation and Training

5.1 Installation Requirements

Installation shall be conducted on-site at the government facility by a highly trained, OEM-certified (preferred) Service Engineer. The installation process must adhere to ISO 9001 quality procedures (or equivalent certified quality management standards) to ensure consistency, high-quality setup, and baseline calibration.

5.2 Facility & Site Preparation Requirements

The contractor shall provide a comprehensive Site Preparation Guide within 15 days of contract award, if required. The equipment provided must be compatible with standard laboratory utility infrastructures, bounded by the following parameters:

Utility / Requirement Maximum Allowable Specification

Electrical Power 110-120V AC, 60Hz or 208V AC, 50/60Hz (Single Phase). System shall utilize standard NEMA 5-15P or equivalent laboratory receptacles.

Footprint & Weight The vendor must define the exact dimensional footprint (W x D x H) and total system weight to ensure compatibility with standard laboratory benches.

5.3 Training Requirements

Upon successful installation and site acceptance testing, the Contractor shall provide a minimum of one (1) day of operator familiarization training for up to six (6) individuals. Training must include:

• Overview of equipment hardware and safe handling of the 3500 Genetic Analyzer and Thermal Cycler.

• Proper loading of samples, Master Mix preparation protocols, and swabbing techniques.

• Basic software navigation (GeneMapper 6), test programming, and data export.

• Routine user-level maintenance and safety procedures.

• User manual (digital storage device or hardcopy).

6.0 Maintenance and Lifecycle Support

6.1 Maintenance Support Plan

To ensure improved instrument performance and increased uptime, the Contractor must offer a comprehensive post-warranty support plan. This coverage shall begin at the end of the standard 1-year warranty period and must include:

• Calibration: DLA ships the calibration standards to the contractor for calibration at no cost OR the contractor schedules a certified technician to come for one (1) on-site visit to perform calibration, as applicable.

• Technical Support: Priority telephone and email troubleshooting support.

• Repair Coverage: Complete corrective repair coverage, including all service, parts, accessories, technical labor, and travel costs.

• Consumables: Discounted pricing on standard consumable purchases for the life of the agreement.

7.0 Administrative Requirements

7.1 Period of Performance

Delivery of all hardware, software, and accessories shall be completed within 90 days after the date of award.

Installation and training shall be coordinated with the technical point of contact upon delivery.

7.2 Pricing

Pricing shall include all costs for shipping and delivery (FOB Destination) to the address specified below.

7.3 Notice of Delivery

Contractor shall give notice to Edward Francoeur and Mike McClain when the instrument is to be delivered.

7.4 Delivery Address / Marking Instructions

Attn: Edward Francoeur DLA Product Test Center 700 Robbins St., Bldg. 5-D Philadelphia, PA 19111

7.5 Invoicing

All invoices must be submitted through Wide Area Workflow (WAWF). See DFARS Clause 252.232-7006 Wide Area Workflow Payment Instructions for detailed instructions. The DoDAAC for this action is SL0701.

8.0 Certification and Verification

8.1 The contractor shall provide instrument certification or verification of instrument performance upon completion of installation (IQ/OQ).

8.2 A NIST Traceable ANSI/NCSL Z540.1 certificate (with data) shall be provided for calibrated components or sub-components, as applicable.

9.0 Warranty

A minimum one-year comprehensive warranty covering all parts, labor, travel, food, and lodging for one service technician must be included.