PWS (Sol).docx

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PACbio Sequencing
Solicitation number
12805B22Q0103
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Performance Work Statement (PWS)

Project Title: Service Agreement for sequencing wheat samples using Pacbio PacBio Sequel II

1. Government Points of Contact

2. Location: HARD WINTER WHEAT GENETICS RESEARCH UNIT

4008 THROCKMORTON HALL, KANSAS STATE UNIVERSITY

MANHATTAN, KS 66506

3. Project Requirements:

We require ~300 Gb processed CCS data per wheat sample that shall need 12 PacBio SMRT Cells per sample. We will submit two samples for total sequencing of 24 cells at a turnaround time of 6 weeks.

4. Description of Work:

The vendor shall conduct quality check of submitted two DNA samples, prepare libraries, conduct sequencing on the library in PacBio Sequel II using HiFi Mode and provide about ~300 GB CCS long reads data per wheat sample.

END

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