PD-Beckman.pdf

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Purchase Description Statement of Need and Purpose:

Background Information and Objective:

Generic Name of Product:

Purchase Description:

Salient Characteristics:

Quantity:

Delivery Date:

Period of Performance:

Option(s):

9.0.0.2.20101008.1.734229

This growing demand has led to the need for additional acoustic dispensers not just for assay ready plates but to be used as a general laboratory instrument for assay development projects beyond just being used to dispense small molecules solubilized in DMSO but nearly any aqueous media. Market research has led us to learn that the only instrument available that can dispense nearly any aqueous media or non-DMSO solvent type without a specific calibration is the LabCyte Echo, meaning a user can walk up to the instrument and start dispensing WITHOUT having to have a custom and SPECIFIC calibration for every liquid type. When we were just using DMSO as our source plate solvent this wasn’t a large issue since we could calibrate for DMSO, now however we have tremendous demand to use a variety of reagent types, be it aqueous solutions, antibodies, cell media, etc. and it takes tremendous effort to create a calibration curve on the EDC and it is not always accurate and once again, is specific to that particular formulation of the reagent. For example, we have an assay that has taken literally a YEAR to get a correct calibration on the EDC to acoustically dispense cell media as the source fluid and even then there are differences from batch to batch.

To address this limitation, the National Center for Advancing Translational Sciences (NCATS) has newly established a Biosafety Level 3 (BSL3) Laboratory to support its ongoing SARS-CoV2 work. To facilitate screening, we propose to purchase (1) Access system that will permit acoustic dispense of compounds, incubation of the assay plates, dispensing of the detection reagents and reading of the plates on a multimode reader. This facilitates high-throughput single agent and combination assays to be performed in 1536-well plate format. This high-containment biological laboratory is required due to the added safety infrastructure required for propagation and handling of SARS-CoV2, including increased air handling requirements and greater security, not currently available within the 9800-laboratory complex.

Overall this project will support ongoing work for small molecule and combination screening, in silico virtual screening, nanobody/antibody design and optimization that are part of NCATS’s ongoing work to support the pandemic response. In addition, we are performing in vitro assays to support ACTIV Trace’s efforts to characterize the response of variant SARS-CoV2 viruses to current vaccine and therapeutic interventions.

Access Workstation Base Echo and Access with 4 integrated devices Installation Service with Basic Operator Training Rear Automation Table Rear Echo Integration Barcode Reader 2D/ID Four Shelf Delidder 20 Plate LD - Random Access Rack NUC 2TB Hard Drive Base extension with shelf 96" Access Table with Echo Turn table ACC-TURNTABLE-65X 65X Series Echo Turntable iKit Centrifuge Device 115V/230V Centrifuge Integration Peeler Device 115V Peeler Shelf Integration Sealer Device 115V Sealer Shelf Integration Cytomat 10C Series Incubator Configurator

100 - 115V UTIL BOX

US/Canada 120V Power Cytomat 10C Incubator 120V Cytomat 10 Barcode Reader Cytomat Stacker P23/21 Cytomat 10C and Hotel Transfer Station Cytomat Next to Echo Right Rear Integration Echo 650 System Liquid Handler for Screening and Omics SU Echo 650 Start-Up Care Service Contract Echo Compressor North America Echo Client Computer Dual Voltage Vacuum Pump 115/230VAC Echo Installation Service without Training Echo Cherry Pick Software 1.7 Echo Dose-Response Software 1.7 Echo Plate Reformat Software 1.7

KIT SMC CHILLER

SW, SSD Upgrade, Echo Dose-Response, 1.7 to 1.8 SW, SSD Upgrade, Echo Cherry Pick, 1.7 to 1.8 Upgrade, Echo Plate Reformat, 1.7 to 1.8 Specify the significant, essential physical, functional, performance, or material element(s) (the distinguishing characteristic) of a product, system, component, or service required. Specify the functions that a system or component must perform to meet the Government’s objectives. Include required levels of performance, features or characteristics of the system or component that are essential to meet the government’s objectives. Specify the Government’s minimum acceptable standards.: 1. Robotic system, scheduling software and peripherals must be fully compatible and integratable with existing Labcyte ECHO 525 and ECHO 650 acoustic dispensers.

2. The system must support a variety of reagent types; including DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents.

3. Must be capable of 24/7 operation.

4. System must include Echo expansion software for cherry picking and IC50. It must have the ability to manipulate cherry picking and IC50 on the Echo via a standard, non-custom interface.

5. System must have integrated, random accessible, automated humidified CO2 incubator for plate storage and incubation.

6. System must be capable of handling, in an automated fashion, plates with lids or foil seals to centrifuge, remove seal, handle liquid dispense, and seal/re-lid post transfer.

7. The acoustic liquid handler must support a variety of reagent types; including DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents.

8. The acoustic liquid handler must NOT require any reagent specific calibration.

9. The instrument can transfer multiple fluid types and volumes from one plate into another plate enabling rapid assay creation from one source plate.

10. Must be a completely non-contact dispense. The instrument uses acoustic energy to transfer fluids. The instrument does not require tips, nozzles, or pin tools during fluid transfer. This eliminates the possibility of samples adhering to the tips, tip carryover, or tip leachates entering the sample.

11. The instrument uses a single transducer to transfer samples acoustically from a source plate to an inverted destination plate.

12. The instrument can transfer reagents in the following ways:

One well to one well for plate replication in 384 and 1536 well patterns.

One well to many wells to distribute solutions to destination plates containing 1 - ≥3456 wells or target locations.

13. Any well to any well which enables cherry picking, hit picking, and reformatting of solutions to destination plates containing 1 - ≥3456 wells or target locations.

14. Must include Echo expansion software for cherry picking and and IC50. The DRP must have the ability to manipulate cherry picking and IC50 on the Echo via a standard, non-custom interface.

Specify quantity. : Qty 1 Access Workstation Base Qty 1 Echo and Access with 4 integrated devices Installation Service with Basic Operator Training Qty 1 Rear Automation Table Qty 1 Rear Echo Integration Qty 1 Barcode Reader 2D/ID Qty 1 Four Shelf Delidder Qty 3 20 Plate LD - Random Access Rack Qty 1 NUC 2TB Hard Drive Qty 1 Base extension with shelf Qty 1 96" Access Table with Echo Turn table Qty 1 ACC-TURNTABLE-65X 65X Series Echo Turntable iKit Qty 1 Centrifuge Device 115V/230V Qty 1 Centrifuge Integration Qty 1 Peeler Device 115V Qty 1 Peeler Shelf Integration Qty 1 Sealer Device 115V Qty 1 Sealer Shelf Integration Qty 1 Cytomat 10C Series Incubator Configurator Qty 1 100 - 115V UTIL BOX Qty 1 US/Canada 120V Power Qty 1 Cytomat 10C Incubator 120V Qty 1 Cytomat 10 Barcode Reader Qty 10 Cytomat Stacker P23/21 Qty 1 Cytomat 10C and Hotel Transfer Station Qty 1 Cytomat Next to Echo Right Rear Integration Qty 1 Echo 650 System Liquid Handler for Screening and Omics Qty 1 SU Echo 650 Start-Up Care Service Contract Qty 1 Echo Compressor North America Qty 1 Echo Client Computer Qty 1 Dual Voltage Vacuum Pump 115/230VAC Qty 1 Echo Installation Service without Training Qty 1 Echo Cherry Pick Software 1.7 Qty 1 Echo Dose-Response Software 1.7 Qty 1 Echo Plate Reformat Software 1.7 Qty 1 KIT SMC CHILLER Qty 1 SW, SSD Upgrade, Echo Dose-Response, 1.7 to 1.8 Qty 1 SW, SSD Upgrade, Echo Cherry Pick, 1.7 to 1.8 Qty 1 Upgrade, Echo Plate Reformat, 1.7 to 1.8