SOW_Attachment_A_-_Rev_9-7-2018.pdf

Attachment A NIEHS Defined Specific Pathogen Free (SPF) Agent Exclusion List

The NIEHS reserves the right to alter this exclusion list based on emerging pathogens or changes in agent pathogenicity

RANK TESTING

FREQUENCY *

NIEHS ORGANISM LIST SPECIES RECOMMENDED TEST

OR SPECIMEN

1 4 wks Minute Virus of Mice (MVM) M Serology, IFA, PCR or Western Blot 1 4 wks Mouse Hepatitis Virus (MHV) M Serology, IFA, PCR or Western Blot 1 4 wks Mouse Norovirus (MNV) M Serology, IFA or PCR or Western Blot 1 4 wks Mouse Parvovirus (MPV) M Serology, IFA, PCR or Western Blot (see additional requirements in SOW)

1 4 wks Mouse Polio Virus (TMEV, MEV, GDVII)

M Serology, IFA, PCR or Western Blot

1 4 wks Rota Virus (Enzootic Diarrhea of Infant Mice - EDIM)

M Serology, IFA, PCR or Western Blot

1 4 wks Rat Parvovirus (RPV) R Serology, IFA, PCR or Western Blot

1 4 wks Rat Minute Virus R Serology, IFA, PCR or Western Blot

1 4 wks Rat Coronavirus (RCV/SDAV) R Serology, IFA, PCR or Western Blot

1 12 wks Cilia-Associated Respiratory (CAR) Bacillus

M, R, G Serology, IFA, PCR or Western Blot

1 12 wks Citrobacter rodentium M, G Fecal Culture or PCR

1 12 wks Clostridium piliforme All Serology, PCR, Histopathology (if indicated)

1 12 wks Corynebacterium kutscheri M, R, H Tracheal/Oral/Nasopharyngeal Culture 1 12 wks Ectoparasites All Low Power Microscopic Examination of

Fur and Skin; PCR

1 12 wks Giardia muris All Proximal Small Intestine 1 12 wks Helicobacter sp. (if positive, speciate) All PCR and/or Culture

(specify screening method in health reports)

1 12 wks Lymphocytic Choriomeningitis Virus (LCMV)

All Serology, IFA, PCR or Western Blot

1 12 wks Mycoplasma pulmonis M, R, H Tracheal/Oral/Nasopharyngeal Culture, Serology, IFA, or /PCR

1 12 wks All Intestinal Helminths All Fecal Concentration or PCR 1 12 wks Pasteurella pneumotropica M, R, H Tracheal/Oral/Nasopharyngeal Culture or PCR 1 12 wks Pneumonia Virus of Mice (PVM) M, R, H Serology, IFA, PCR or Western Blot 1 12 wks Rat Theilovirus (RTV) or TMEV-like agent R Serology, IFA, PCR or Western Blot

1 12 wks Reo Virus - Strains 1-3 M, R, H Serology, IFA, PCR or Western Blot 1 12 wks Rodentolepsis nana Fecal Concentration 1 12 wks Salmonella species All Fecal Culture

1 12 wks Simian Paramyxovirus Type 5

(SV5)

H Serology, IFA, PCR or Western Blot

1 12 wks Sendai Virus M, R Serology, IFA, PCR or Western Blot 1 12 wks Spironucleus muris M, R Proximal Small Intestine Wet Mount 1 12 wks Streptobaccillus moniliformis M, R Tracheal/Oral/Nasopharyngeal Culture

1 12 wks Streptococcus pneumoniae R Tracheal/Oral/Nasopharyngeal Culture

1 26 wks Ectromelia Virus M Serology, IFA, PCR or Western Blot 1 26 wks Encephalitozoon cuniculi M, R Serology, IFA, PCR or Western Blot 1 26 wks Hanta Virus (Testing quarterly for international shipments or animals imported from outside sources)

M, R Serology, IFA, PCR or Western Blot

1 26 wks Lactate Dehydrogenase- Elevating Virus (LDHV)

M Serology, IFA, PCR or Western Blot

1 26 wks Mouse Adenovirus (MAV) M, R Serology, IFA, PCR or Western Blot 1 26 wks Mouse Cytomegalovirus

(MCMV)

M Serology, IFA, PCR or Western Blot

1 26 wks Mouse Thymic Virus (MTV) M Serology, IFA, PCR or Western Blot 1 26 wks Polyoma Virus M Serology, IFA, PCR or Western Blot 1 26 wks Mouse Pneumonitis Virus (K) M Serology, IFA, PCR or Western Blot 1 26 wks Polyoma Virus M Serology, IFA, PCR or Western Blot 1 necessary only if ectoparasites present

Eperythrozoon coccoides Stained Blood Smears, Smears prepared from splenectomized mice

1 necessary only if ectoparasites present

Hemobartonella muris M Stained Blood Smears, Smears prepared from splenectomized mice

2 12 wks Klebsiella oxytoca M Tracheal/Oral/Nasopharyngeal Culture;

PCR

2 12 wks Klebsiella pneumoniae M Tracheal/Oral/Nasopharyngeal Culture;

PCR

2 12 wks Other Protozoa All Fecal Wet Mounts

2/3 12 wks Bordetella bronchiseptica M, R, H Tracheal/Oral/Nasopharyngeal Culture or PCR

2/3 12 wks Corynebacterium bovis M Skin culture or PCR 2/3 12 wks Coagulase (+) Staphylococcus species All Tracheal/Oral/Nasopharyngeal Culture or PCR 2/3 12 wks Pseudomonas aeruginosa All Tracheal/Oral/Pharyngeal Culture and

Fecal Culture or PCR

2/3 12 wks Pneumocystis murina/carinii (immuno-deficient mice)

M, R Lung, Histopathology or Immunochemistry or PCR; Serology (if available)

RANKING CATEGORY AS IT APPLIES TO ISSUING ANIMALS TO ANY NIH DELIVERY ADDRESS

1. Vendor shall not issue animals to NIEHS from rooms/units/barriers positive for these agents without a specific written request demonstrating acceptance from the ICD Veterinarian acknowledging the positive status on the face of the order.

2. Vendor may issue animals, with pre-approval from NIEHS COR or designee, from areas known to be positive for these agents if no alternate clean source is available unless a specific request for animals free of these agents are present on the face of the order form. (Vendors shall continue to emphasize maintenance of colonies free of these agents.)

3. For Immunodeficient animals, these agents shall be interpreted at the criteria stated in ranking category 1.

* Four weeks may be interpreted as monthly, twelve weeks may be interpreted as quarterly, twenty-six weeks may be interpreted as semi-annually, and 52 weeks may be interpreted as annually.

Species M = Mouse R = Rat H = Hamster G = Gerbil All = Mouse, Rat, Hamster, Gerbil, Guinea Pig