Mirava Product List.pdf

PRODUCTS LIST

1. MIRAVA STED AND Confocal Core Microscope – Qty (1) EA

The MIRAVA Core platform is the cornerstone of your advanced microscopy system. Designed for unparalleled precision, flexibility, and ease of use, it empowers you to elevate your research to unprecedented levels. It not only serves as the foundation for more advanced configurations but also functions as a STED and confocal microscope on its own. From here, additional abberior modules can be integrated, each adding another superpower to your

MIRAVA to give you exactly what you need to acquire the perfect image. With its user-friendly interface and versatile expansion options, this platform is ideal for cutting-edge research in biology, medicine, and materials science. Begin your journey into the world of super resolution microscopy with a foundation that offers everything you need and more.

◘ Motorized research inverted microscope body

Olympus / Evident IX83 (2-deck version) featuring as follows.

• Transmitted Koehler illuminator (halogen lamp)

• Motorized nose piece and filter wheels

• UPLXAPO60xO, WD: 0.15, NA: 1.42, Objective lens for epifluorescence, confocal and STED mode

• Motorized XY-stage including universal sample holder

(well plates, 35mm dishes, and slides). Fully software integrated

• 200μm piezo z-stage with universal sample holder for precise and fast z-stacks

◘ Ergonomic Workbench and Microscope Table with

Active Damping featuring as follows.

MIRAVA table frame with integrated active vibration isolation for six degrees of freedom without the need for compressed air supply

• High-stability, high-stiffness recessed optical breadboard with optical enclosure for superior suppression of unwanted stray light or reflected light

• Ergonomic workbench with computer desk

◘ 4 Channel excitation laser unit with 640nm, 561nm, 485nm and 405nm:

• Fluorescence Excitation Laser Module 405 nm (+-5 nm, CW), 20mW output power @40MHz, fast power control with external AOM

• Pulsed fluorescence excitation laser module 485 nm

(+-5 nm, pulsed), 1mW output power @40MHz, fast power control with external AOM

• Pulsed fluorescence excitation laser module 561 nm

(+-5 nm, pulsed), 200μW output power @40MHz, fast power control by direct trigger modulation

• Pulsed fluorescence excitation laser module 640 nm

(+-5 nm, pulsed), 1mW output power @40MHz, fast power control with external AOM

• Motorized mirrors for auto-alignment

• Highly efficient narrow notch filters completely removable from beampath when laser is not in use, resulting in a high-transmission, gap-free detection spectrum - automatically set by software

◘ Patent protected QUADScan beam scanning technology

• Field number: 11.3

• Maximum image size: 16000 x 16000 or 256 Mpixels

• Point scanner with a line frequency of up to 2.6 kHz;

data collection of up to 4.2 frames per second for 512 x 512 pixels

◘ 3 Channel spectral detection unit with super-sensitive single photon detectors as follows.

• Three spectral detection modules freely tunable between 400nm and 800nm

• Three single photon detectors (APDs) with superior quantum efficiency of up to 65%, extremely low dark counts (<250 Hz) to render finest details and high time resolution for gated detection in confocal and STED mode

• Motorized pinhole with 18 diameters and motorized alignment integrated in the auto-alignment routine

◘ High-power 3 Watt 775nm STED laser with easy3D module

• STED Laser Module 775nm, output power >2750mW

@ 40MHz, repetition rate 25-40MHz

• High-power AOM on 3-axis mount for ultrafast power

Control

• Easy3D module via single-beam design for shaping ideal PSFs for 2D and 3D STED using spatial light modulator technology, seemless control of light distribution for 2D and 3D STED.

• STED resolution <100x100x100 nm in 3D and

<30x30 nm in 2D (typically 80x80x90 nm and 25x25 nm, respectively) for UPLXAPO60XO objective lens

◘ Electronic workstation with high-end PC, FPGA and monitor as follows:

• Fast PC-Workstation (min. AMD Ryzen 7 5800X 8-core CPU, up to 4.5 GHz, 128 GB DDR4 RAM, Nvidia

GTX-5070 with 12GB VRAM, 2TB NVMe SSD, 2TB

SSD, and 2.5GB ethernet. Monitor: Lenovo ThinkVision

T32h-20 or similar)

• FPGA-based control board with upgradable firmware with multiple addressable IO channels: 24AO, 8AI, 40DIO, 8 laser trigger, 2 sync inputs, 9 counter inputs, Firmware package with entire control of the microscope and collection of signal in up to 16 timing or gating channels for quantitative measurements. Firmware ready for FLIM and TimeBow with additional TCSPC hardware

◘ LED 16 Color Widefield Illumination on rear port, condenser, turret with Quadband Filter

• Quad band filter cube (DAPI/FITC/Cy3/Cy5)

• LED spectrum: 365-770 nm, with 16 LEDs

• Four channel wavelength-grouping design matches most commercially available dual, triple, and quad filters

• Individual control of selected LED wavelengths reduces background

• Instant on/off: No shutters required, no warm up or cool down

• Stable and repeatable: reliable and consistent results

• Precise irradiance control in 1% steps (0-100%): no

ND filters required

◘ LiGHTBOX software suite for a ease-of use control of the microscope hardware, data acquisition and analysis tool boxes

• Easy to use for beginners as well as expert users. Get a STED image in three clicks

• Intuitive experiment planning, from smart selection of used lasers, detection windows and imaging parameters such as pinhole and pixelsize

• Progressive disclosure allows advanced users full control, while new users can start immediately without training due to the intuitive workflow

• Highly automated settings engine keeps nonessential parameters away from the user unless specifically requested

• Smart settings enable optimized imaging sequences through line or frame steps with automated selection of detectors

• Images and samples are held in a spatial context, e.g.

between overview images and closeup recordings

• Very fast 1-click switching between imaging parameter sets, for example a set for fast panning and one for super resolution imaging

• Intuitive drag & drop for quick transfer of parameter sets between single or multiple images, also for easy repetition of experiments

• Intuitive data display, analysis and 3D rendering

• Timelapse, frame steps, line steps and multiposition measurements including tiling and stitching

• Processing, annotation and quantification

• 3D viewer with video export function

• Quick export function for instant publication-ready figures

• TRUESHARP image boosting deconvolution. It removes noise and background signal from your recorded data and strongly enhances resolution, sharpness, and brilliance of your STED or confocal images by incorporating experimentally measured information

◘ FLEXPOSURE Adaptive Illumination Package for

Highest Resolution and Live-cell Imaging

• Light dose minimization by up to two orders of magnitude to leverage live cell imaging

• Bleaching reduction applicable to all fluorescent markers

• Ultra-high resolutions down to 25 nm (2D) or 75 nm

(3D) (depends on sample and dye; only with high power laser upgrade)

• Signal enhancement by one order of magnitude

(depends on sample and dye)

• Based on concurrent, pixel-wise, intelligent adaptiation of the STED-power required for reaching maximal resolution with minimal light dosages

• Easy to use LightBox software interface; specially illuminated areas can be highlighted and analyzed. All parameters can be applied in a continuous fashion

• Using MINFIELD imaging modality, ultra-high resolutions down to 20 nm (2D) or 65 nm (3D)

(depends on sample, scan field, and dye; only with high power laser upgrade) achievable in various samples

• Signal enhancement by orders of magnitude

(depends on sample, scan field, and dye)

• Applicable to all fluorescent markers

◘ Dye & sample bundle for an easy and efficient usage of the microscope

• 2 x 100μl each of abberior STAR ORANGE, STAR

GREEN and STAR RED each as goat anti-mouse IgG and goat anti-rabbit IgG

• 10 x 1ml each of Abberior Mount Solid antifade and

Mount Liquid antifade

• abberior NANOPARTICLE AUTOALIGNMENT SLIDE

• 3 microscope slides (ready-to-image):

NANOPARTICLES GOLD 150 nm + 2C FLUOR 120 nm, NANOPARTICLES RED FLUOR 40 nm, NANOPARTICLES GREEN FLUOR 40 nm

• 5 microscope slides (ready-to-image):

NANOPARTICLES 2C FLUOR 120 nm, NANOPARTICLES RED FLUOR 40 nm, CELLS 1C

NPC-abberior STAR RED, CELLS 2C NPC abberior

STAR RED / Golgi-abberior STAR ORANGE, CELLS 4C

NPC-abberior STAR RED / Golgi-abberior STAR

ORANGE / Phalloidin-abberior STAR GREEN / DAPI

2. Evident / Olympus Objective lens 20X, dry immersion, long working distance

• Olympus/Evident LUCPLFLN20X2 objective lens

• working distance of 6.6 to 7.8 mm

• correction collar

• NA 0.45, 20X magnification

• integration into measurement software

3. RAYSHAPE: Adaptive Optics System for MIRAVA - Qty (1) EA

• Deformable mirror-based correction of arbitrary aberrations for all beams

• Including software package

• More than 140 elements with a stroke of several microns ensure precise correction, even of high-order aberrations

• Compensates for sample-induced aberrations, e.g.

when focusing into a refractive-index mismatched sample, or into samples with internal inhomogeneities

• Applied spherical correction changes with focus to ensure automatic correction at all focal depths

• No moving elements in the objective

• Enables superior signal and resolution, in particular with 3D-STED, but also for confocal and multiphoton images, e.g. of thick tissue samples

4. MATRIX detector upgrade for one APD channel – Qty (1) EA (base, 1st Matrix)

• Enables dramatic reduction of background levels on a the basis of physics - no estimation procedure sch as deconvolution. MATRIX detects the background for each pixel in the STED image and allows the operator to subtract it ("differential detection"). The final image is greatly suited for further processing and analysis, e.g.

deconvolution, particle counting, etc.

• Exclusive technology from abberior.

• Combines with pixel reassignment and our intelligent array deconvolution algorithm for even better resolution enhancement.

• Fully compatible with spectral RAINBOW detection.

Detection bands can be continuously selected between 400 and 800 nm.

• ~50% quantum efficiency (@ 500 nm)

• Contains one MATRIX array detector with more than 20 avalanche photodiodes in single photon counting mode

• Full software integration

5. Handling, Packing, Shipping and Insurance (North America) – Qty (1) EA

6. Installation North America – Qty (1) EA

7. Training 2 Days on-site – Qty (1) CHG

- 2 Day on-site training for Abberior Instrument products

- Review of theory -> to practical hands on sessions on the machine

- Includes all travel and expense for Applications Person