PUR 7776 (Revised 07/2022) Page 1 of 3

Incorporated by Reference: Rule 60A-1.045, F.A.C.

DESCRIPTION OF INTENDED

SINGLE SOURCE PURCHASE

(PUR 7776)

AGENCY: Florida Fish and Wildlife Conservation Commission

TITLE: Advanced Microscopy Techniques Transmission Electron Microscope camera system

Short description of the commodity or service desired: Scientific grade complementary metal oxide semiconductor (CMOS) camera that is fully compatible with our existing Transmission

Electron Microscope (TEM) model JEM 1400 and that includes all specifications needed to achieve the desired detection sensitivity for our applications

CONTACT

Name: Martin Ehlen

Address: 1875 Orange Avenue East, Tallahassee, FL 32311-6160

Telephone: 850-617-9602

Email: martin.ehlen@myfwc.com

Internal tracking number, if any: FWC SS 25/26-15

Date posted: 4/17/2026 Last day for receipt of information: 5/8/2026

This description of commodities or contractual services intended for purchase from a single source is posted in accordance with Sections 120.57(3) and 287.057(3), Florida Statutes (F.S.), and will remain posted for a period of at least 15 business days.

Commodity or Contractual Service Required (commodity or United National Standard Products and Services Code (UNSPSC), manufacturer, model, and description, as appropriate):

81101706: Laboratory equipment maintenance

81141504: Equipment test calibration or repair

41116500: Instrument parts and accessories

41100000: Laboratory and scientific equipment

41111706: Photo attachments for microscopes

41104000: Sampling equipment

41111721: Transmission electron microscopes

Quantity or Term (as appropriate):

3 years

Requestor (division, bureau, office, individual, as appropriate):

Fish and Wildlife Research Institute, Stephanie Keller Abbe, Research Associate

Performance and/or Design Requirements (e.g. intended use, function or application, compatibility, requirements; reference to policy, rule, statute or other act of the Legislature, etc., as appropriate):

PUR 7776 (Revised 07/2022) Page 2 of 3

Incorporated by Reference: Rule 60A-1.045, F.A.C.

Minimum specifications for high-resolution imaging on our transmission electron microscope

(TEM) requires a scientific grade complementary metal oxide semiconductor (CMOS) camera that is fully compatible with our existing TEM model JEM 1400 and that includes all specifications needed to achieve the desired detection sensitivity for our applications, as follows: phosphor scintillator coupled by a finite conjugate macro lens that is optimized for the phosphor spectrum with a lens input numerical aperture exceeding 0.3; phosphor pixels at a minimum of 8.5 µm x 8.5 µm in physical size; lens modulation transfer function at the CMOS Nyquist frequency at a minimum of 50% across the entire field of view; minimum of 5000 pixels in the horizontal direction and a minimum of 2900 pixels in the vertical direction; and image acquisition at a minimum of 16-bits, with x2 and x4 binning supported for increased sensitivity with a maximum frame rate of 30 fps or more. Full functionality requires that the package must provide software for image acquisition and processing that includes all of the following: auto-numbering and file generation for efficient acquisition of case studies; data generation (automatically imbedded) such as magnification, kV and stage position; and image files with user generated graphics such as captions, text and measurement directly saved in standard JPEG or TIFF formats without the need for image format conversion. Moreover, all software and hardware should be compatible with the

Windows 10 and Windows 11 operating systems and be able, when necessary, to communicate with our JEM 1400’s control system via a dedicated Ethernet link used to control TEM functions.

The camera should also support SerialEM for tomography, montaging and single particle analysis critical to our electron microscopy applications.

Intended Single Source:

Advanced Microscopy Techniques

Estimated Dollar Amount:

Up to $150,000

Justification for single source acquisition (what is necessary and unique about the product, service or source; steps taken to confirm unavailability of competition, as appropriate):

Advanced Microscopy Techniques (AMT) is the only vendor that can provide a scientific grade complementary metal oxide semiconductor (CMOS) camera that is fully compatible with our existing transmission electron microscope (TEM) model JEM 1400 and that includes all specifications required to achieve the desired detection sensitivity for our applications. The

NanoSprint15B achieves high sensitivity, fine-pixel, low noise CMOS sensor that offers a large

15-megapixel sampling region with a high speed 30fps readout. AMT is the sole distributor of the

NanoSprint15B.

Approved By (names & titles, as appropriate, e.g., requestor, requestor management, information systems, budget, purchasing):

Stephanie Keller Abbe (Research Associate, FWRI)

Stephen Pollett (OMC 1 Fiscal Asst., FWRI)

Katherine Hubbard (Research Scientist/Administrator II, FWRI)

Caroline Gorga (Section Leader, FWRI)

Leanne Flewelling (Deputy Director, FWRI)

Joshua Stringer (OMC Manager, OED)

Rachel Korade (General Services Purchasing Director, OED)

PUR 7776 (Revised 07/2022) Page 3 of 3

Incorporated by Reference: Rule 60A-1.045, F.A.C.

Prospective vendors are requested to provide information regarding their ability to supply the commodities or contractual services described. If it is determined in writing by the agency, after reviewing any information received from prospective vendors, that the commodities or contractual services are available only from a single source, the agency shall provide notice of its intended decision to enter a single-source purchase contract in the manner specified in Rule 60A-1.045, Florida Administrative Code, and section 120.57(3), F.S.