Sources Sought_Inverted Fluorescence Microscope_FA700026Q0062_Amendment 1.pdf

SOURCES SOUGHT AMENDMENT 01

Inverted Fluorescence Microscope

Notice Type: Sources Sought – Amendment 01

Response Deadline: 30 June 2026, 10:00 AM MDT

NAICS Code: 333310 – Commercial and Service Industry Machinery Manufacturing

Product Service Code (PSC): 6650 – Optical Instruments, Test Equipment, Components, and Accessories

Table of Contents Table of Contents

1. Purpose of Amendment

2. Updated Minimum Salient Characteristics

2.1 System Type and Base Capabilities

2.2 Illumination and Contrast

2.3 Objectives and Optics

2.4 Fluorescence Capabilities

2.5 Stage and Sample Handling

2.6 Digital Imaging and Software

2.7 Support and Logistics

Inverted Fluorescence Microscope | Sources Sought Amendment 01

1. Purpose of Amendment The purpose of this amendment is to update the Minimum Salient Characteristics for the required Inverted Fluorescence Microscope. All other terms, conditions, and submission instructions from the original Sources Sought notice remain unchanged.

2. Updated Minimum Salient Characteristics To be considered capable of meeting this requirement, the proposed equipment must meet or exceed the following brand-agnostic minimum specifications:

2.1 System Type and Base Capabilities

• Modular research-grade inverted microscope stand with a defined, easy path for future upgradability.

• Motorized, high-precision Z-focus mechanism for parfocality and long-term stability.

• Motorized automated objective nosepiece/turret (minimum 6-position capacity).

• External touch-screen control interface and/or smart multi-function control boxes for system operation.

2.2 Illumination and Contrast

• Motorized, tiltable transmitted light arm with LED transmitted light source.

• Fully motorized Differential Interference Contrast (DIC) capabilities for generating high-quality cell images (including motorized DIC turret, condenser, and polarizer).

• High-intensity incident light fluorescence provided by a broad-spectrum LED light source (delivered via fluid light guide or equivalent).

2.3 Objectives and Optics

• Infinity-corrected optical path with large field-of-view eyepieces for sample overviews (e.g., 22mm

FOV or greater).

• High-performance, fluorescence-grade objective lenses.

• Required magnifications: 10x, 20x, 40x, and 60x (or 63x) capabilities (including appropriate phase/fluorite/oil immersion specifications for high-resolution imaging).

2.4 Fluorescence Capabilities

• Motorized fluorescence filter turret (minimum 6 positions) with fluorescence protection shield.

• Fluorescence filter cubes/sets compatible with standard biological stains, including DAPI, GFP/FITC, and Texas Red (TXR).

Inverted Fluorescence Microscope | Sources Sought Amendment 01

2.5 Stage and Sample Handling

• High-precision motorized X-Y scanning stage.

• System must include a dedicated anti-vibration table specifically designed to ensure long-term stability during automated imaging.

2.6 Digital Imaging and Software

• Motorized camera side port with 1x C-mount adapter.

• High-resolution, high-sensitivity scientific monochrome camera (e.g., sCMOS) for fluorescence imaging.

Computational Clearing: The included image analysis software must feature an advanced computational clearing algorithm (opto-digital technology) designed to remove out-of-focus blur and haze from widefield images in real-time or post-processing for the generation of the highest quality images.

• Dedicated high-performance processing workstation/PC to run the software and process images.

2.7 Support and Logistics

• In-person, on-site installation by a qualified field service engineer.

• In-person training and post-installation support.

• Standard manufacturer warranty.