eDNA_Filter_Lab_Testing_Services_SOW_020624.docx

Business and Scope of Work – DNA analysis

This invitation for bid is a request is for a minimum of one lab and a maximum of two labs to provide the following services:

· qPCR analyses of filter disks for the presence of environmental DNA with the option to select one to several different species including but not limited to Zebra and Quagga (Dreissenid) mussels New Zealand mudsnails, Corbicula, and fish species.

· Option to expedite processing for some samples for an additional surcharge.

· DNA analyses of suspect mussel species found during processing samples through microscopy or of other species from other sampling methods.

· Novel qPCR Diagnostic Assay Design and Validation as needed for listed species to be tested.

· With prior agreement between parties, return of unused samples or sample remnants not consumed during the analysis process.

Estimated Contract Usage:

The total contract value cannot exceed $199,996. The number of samples to be analyzed each contract year is estimated at 350-400 samples; however, there is no guarantee of any minimum number of samples; and the number of samples may also exceed 350-400 some years as need arises. Samples will be submitted on an “as needed” basis utilizing the total cost per sample rate provided by the Contractor. After the contract’s initial term and if State agrees to a renewal, the parties may negotiate cost adjustments at the time of contract renewal. Any cost increases must be based on demonstrated industrywide or regional increases in Contractor's costs. State is not obligated to agree upon a renewal or a cost increase but expects a total contract length to not exceed 4 years.

Estimated Fee Schedule: The contractor must complete a blank fee schedule following the estimated feed schedule listed below with relevant prices. The following fee schedule breakdowns the estimated cost for each service. The total contract value of $199,996 may include different combinations of each service and species tested each year.

Technical Specifications:

The following is a list of the technical specifications for DNA sample analysis for presence/absence detection:

Contractor must provide laboratory testing services per the following:

· Contractor must have a minimum of five years of experience conducting contracted DNA qPCR processing of eDNA samples. The Contractor must validate that they have at least five years of experience by providing a statement with their quote affirming they meet this requirement.

· Contractor must conduct DNA qPCR analysis on filter disks for the presence/absence of selected species using established qPCR methods. Contractor must have the knowledge and experience to identify listed species utilizing established qPCR methods. Contractor also must follow standard quality assurance/quality control (QA/QC) protocols utilizing both positive and negative controls.

· Contractor must run all eDNA samples in triplicate qPCR reactions. If two of three replicates are positive the sample is scored as positive. If only one replicate is positive the sample will be rerun in a second qPCR run (again in triplicate). If one replicate in the second run (rerun) is positive the sample is scored as positive. If none of the replicates in second run (rerun) are positive, then the sample is scored as negative. Other processes must be approved by FWP for contract eligibility.

· eDNA samples, where available, may be processed and tested for:

· Zebra/Quagga (combined species) (Dreissena polymorpha/ Dreissena bugensis)

· New Zealand mudsnail (Potamopyrgus antipodarum)

· Corbicula clam (Corbicula fluminea)

· Westslope cutthroat trout (Oncorhynchus clarkii lewisi)

· Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri)

· Rainbow trout (Oncorhynchus mykiss)

· Brook trout (Salvelinus fontinalis)

· Salmoninae sub-family

· Specimen genetic testing, where available, may include testing for:

· Zebra/Quagga (combined species) (Dreissena polymorpha/ Dreissena bugensis)

· New Zealand mudsnail (Potamopyrgus antipodarum)

· Corbicula clam (Corbicula fluminea)

· Eurasian watermilfoil (Myriophyllum spicatum)

· Rusty crayfish (Faxonius rusticus)

· Common reed (Phragmites australis ssp australis/ Phragmites australis ssp americanus)

· Starry stonewort (Nitellopsis obtusa)

· FWP maintains ownership of any samples and may request any untested or sample remnants be returned to FWP or sent to a different lab for verification.

· Assays used must be publicly available, made public, or at a minimum, in the event any results come back positive, the contractor must provide the assay used to verifying lab for its use. FWP may cover the lab’s cost to develop assays so lab can perform test for species that are not publicly available in return they are made available to FWP to use as needed in the future.

· Contractor must provide highly trained personnel with extensive experience with species identification utilizing established and accepted DNA qPCR analysis methods to process eDNA samples.

· Contractor must provide species ID/verification using established genetic analysis methods.

· Contractor must be capable of receiving samples in coolers via FedEx, UPS, or hand delivery.

· Contractor must provide sample results by email using a form provided by FWP or other FWP-approved form within 14 business days of receiving the sample. The sample results must include a sample report indicating the samples that were analyzed using FWP location and date information provided on the sample bottle and a detailed summary of analysis conducted and results obtained.

· Expedited Samples: Contractor, must provide sample results by email using a form provided by FWP or other FWP-approved form within 2 business days of receiving the sample. The sample results must include a sample report indicating the samples that were analyzed using FWP location and date information provided on the sample bottle and a detailed summary of analysis conducted and results obtained.

Contractor must use the following laboratory equipment to perform eDNA laboratory testing services:

· State of the art qPCR analysis equipment.

Data Confidentiality:

Contractor must sign a Nondisclosure Agreement to provide the results of any and all tests to FWP only and not share this information with any other party unless written consent is granted from FWP. Contractors that do not provide a signed Nondisclosure Agreement will be considered non-responsive and will not be considered for award.

I ______________________acknowledge that __________________________ complies with the above Scope of Work, and if awarded, will perform these duties as required.

___________________________________________ Contractor Name

___________________________________________ Name

___________________________________________ Title

__________________________________________ Signature