1207444-event.pdf

26 March 2024

PRC Molecular Testing of eDNA or Specimens PRC Molecular Testing of eDNA or Specimens

26 March 2024

Open 2/7/2024 10:00 AM MST Type Invitation For Bid Close 3/7/2024 4:00 PM MST Number FWP-24-0161 Currency US Dollar

Sealed Until 3/8/2024 1:30 PM MST

Payment Terms 0% 0, Net 30

26 March 2024

Contacts Pamela Hemminger Pamela.Hemminger@mt.gov

Phone +1 406-495-3276

26 March 2024

Commodity Codes Commodity Code Description 3003 Laboratory - Services 3202 Livestock, Fish & Game - Animal DNA Testing 3203 Livestock, Fish & Game - Aquatic Biology Services

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This invitation for bid is a request is for a minimum of one lab and a maximum of two labs to provide the following services:

• qPCR analyses of filter disks for the presence of environmental DNA with the option to select one to several different species including but not limited to Zebra and Quagga (Dreissenid) mussels New Zealand mudsnails, Corbicula, and fish species.

• Option to expedite processing for some samples for an additional surcharge.

• DNA analyses of suspect mussel species found during processing samples through microscopy or of other species from other sampling methods.

• Novel qPCR Diagnostic Assay Design and Validation as needed for listed species to be tested.

• With prior agreement between parties, return of unused samples or sample remnants not consumed during the analysis process.

Description

Required to View Event

Prerequisites Required to Enter Bid

26 March 2024

1.

Please review and accept the State of Montana standard terms and conditions identified herein. Bidders requesting additions or exceptions to the standard terms and conditions must submit them to the procurement officer by the Q&A deadline. The State reserves the right to address nonmaterial requests for exceptions to the standard terms and conditions and contract language with the awarded vendor during contract negotiation.

2. Please review and accept warranties language for services

3. Please review all question and answer entries for this solicitation by clicking on the Q&A Board and viewing each question and answer.

4. Review and accept the Single Point of Contact information.

5. Review and accept the contract as provided.

6. Review and accept the Q & A Board information.

7. Review and accept the Montana Time Zone information.

8. Review and accept the Registration with the Secretary of State information.

9. Review and accept the Debarment information

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Buyer Attachments

1. eDNA_Filter_Lab_Testing_Services_SOW_020624.docx

26 March 202426 March 202426 March 202426 March 202426 March 202426 March 2024 https://bids01.jaggaer.com/apps/Router/PublicEventDownload?file=U291cmNpbmdldmVudC8xMjA3NDQ0LTE1NjEzMTM5NTNlRE5BX0ZpbHRlcl9MYWJfVGVzdGluZ19TZXJ2aWNlc19TT1dfMDIwNjI0LmRvY3g%3D&auth=MDpBRVMyI0NQNVp4a2IvSnkxZDNIUm81TGpka2h4M0V4bkpBZDlVSkVFUnk5WnZrK2l5eGZjOVRGNkRHUlU9

Questions Required Questions

Group 1: Contractor Information Instructions:

1.1 Provide your full company name, address, and phone number. In addition, list the contact(s) for your company who can answer questions regarding your submitted response. Include contact(s) name, phone number, and email address.

1.2 Bidder must download and review the attached Scope document. Thoroughly address each item in the document and upload it to this question.

1.3 Bidder must download and review the attached Cost Proposal document. Thoroughly address each item in the document and upload it to this question.

1.4 Contractor must have a minimum of five years of experience conducting contracted DNA qPCR processing of eDNA samples. The Contractor must validate that they have at least five years of experience by providing a statement with their quote affirming they meet this requirement.

1.5

•Contractor must conduct DNA qPCR analysis on filter disks for the presence/absence of selected species using established qPCR methods. Contractor must have the knowledge and experience to identify listed species utilizing established qPCR methods. Contractor also must follow standard quality assurance/quality control (QA/QC) protocols utilizing both positive and negative controls.

1.6

Contractor must run all eDNA samples in triplicate qPCR reactions. If two of three replicates are positive the sample is scored as positive. If only one replicate is positive the sample will be rerun in a second qPCR run (again in triplicate). If one replicate in the second run (rerun) is positive the sample is scored as positive. If none of the replicates in second run (rerun) are positive, then the sample is scored as negative.

Other processes must be approved by FWP for contract eligibility.

1.7 eDNA samples, where available, may be processed and tested for:

oZebra/Quagga (combined species) (Dreissena polymorpha/ Dreissena bugensis) oNew Zealand mudsnail (Potamopyrgus antipodarum) oCorbicula clam (Corbicula fluminea) oWestslope cutthroat trout (Oncorhynchus clarkii lewisi) oYellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) oRainbow trout (Oncorhynchus mykiss) oBrook trout (Salvelinus fontinalis) oSalmoninae sub-family

1.8

Specimen genetic testing, where available, may include testing for:

oZebra/Quagga (combined species) (Dreissena polymorpha/ Dreissena bugensis) oNew Zealand mudsnail (Potamopyrgus antipodarum) oCorbicula clam (Corbicula fluminea) oEurasian watermilfoil (Myriophyllum spicatum) oRusty crayfish (Faxonius rusticus) oCommon reed (Phragmites australis ssp australis/ Phragmites australis ssp americanus) oStarry stonewort (Nitellopsis obtusa)

1.9 FWP maintains ownership of any samples and may request any untested or sample remnants be returned to FWP or sent to a different lab for verification.

1.10

Assays used must be publicly available, made public, or at a minimum, in the event any results come back positive, the contractor must provide the assay used to verifying lab for its use. FWP may cover the lab’s cost to develop assays so lab can perform test for species that are not publicly available in return they are made available to FWP to use as needed in the future.

1.11 Contractor must provide highly trained personnel with extensive experience with species identification utilizing established and accepted DNA qPCR analysis methods to process eDNA samples.

1.12 Contractor must provide species ID/verification using established genetic analysis methods.

1.13 Contractor must be capable of receiving samples in coolers via FedEx, UPS, or hand delivery.

1.14

•Contractor must provide sample results by email using a form provided by FWP or other FWP-approved form within 14 business days of receiving the sample. The sample results must include a sample report indicating the samples that were analyzed using FWP location and date information provided on the sample bottle and a detailed summary of analysis conducted and results obtained.

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1.15

Expedited Samples: Contractor, must provide sample results by email using a form provided by FWP or other FWP-approved form within 2 business days of receiving the sample. The sample results must include a sample report indicating the samples that were analyzed using FWP location and date information provided on the sample bottle and a detailed summary of analysis conducted and results obtained.

1.16 Contractor must use the following laboratory equipment to perform eDNA laboratory testing services:

•State of the art qPCR analysis equipment.

1.17

Data Confidentiality:

Contractor must sign a Nondisclosure Agreement to provide the results of any and all tests to FWP only and not share this information with any other party unless written consent is granted from FWP. Contractors that do not provide a signed Nondisclosure Agreement will be considered non-responsive and will not be considered for award.

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Product Line Items Product Line Items

There are no Items added to this event.

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Service Line Items Service Line Items

1.

# Item Name, Commodity Code, Description Allow

Alternates Qty. UOM Requested Service Delivery

S1 eDNA qPCR - filter sample 1

Zebra/Quagga (combined species) (Dreissena polymorpha/ Dreissena bugensis)

Turnaround

S2 eDNA qPCR - filter sample 1

New Zealand mudsnail (Potamopyrgus antipodarum)

Standard Turnaround

S3 eDNA qPCR - filter sample 1

Corbicula clam (Corbicula fluminea)

S4 eDNA qPCR - filter sample 1

Westslope cutthroat trout (Oncorhynchus clarkii lewisi

S5 eDNA qPCR - filter sample 1

Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri)

S6 eDNA qPCR - filter sample 1

Rainbow trout (Oncorhynchus mykiss)

S7 eDNA qPCR - filter sample 1

Brook trout (Salvelinus fontinalis)

S8 eDNA qPCR - filter sample 1

Salmoninae sub-family

S9 eDNA qPCR - filter sample 1

Add additional species test to same sample where possible

S10 eDNA qPCR - filter sample 1

EXPEDITED

S11 eDNA qPCR - filter sample 1

EXPEDITED

S12 eDNA qPCR - filter sample 1

Corbicula clam (Corbicula fluminea)

EXPEDITED

S13 eDNA qPCR - filter sample 1

Westslope cutthroat trout (Oncorhynchus clarkii lewisi)

EXPEDITED

S14 eDNA qPCR - filter sample 1

Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri

EXPEDITED

S15 eDNA qPCR - filter sample 1

Rainbow trout (Oncorhynchus mykiss)

EXPEDITED

S16 eDNA qPCR - filter sample 1

Brook trout (Salvelinus fontinalis)

EXPEDITED

S17 eDNA qPCR - filter sample 1

Salmoninae sub family

EXPEDITED

S18 eDNA qPCR - filter sample 1

Add additional species test to same sample where possible

EXPEDITED

S19 Identification or verification with genetic analysis of suspect specimens 1

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S20 Identification or verification with genetic analysis of

S21 Identification or verification with genetic analysis of

Corbicula clam (Corbicula fluminea)

S22 Identification or verification with genetic analysis of

Eurasian watermilfoil (Myriophyllum spicatum)

S23 Identification or verification with genetic analysis of

Rusty crayfish (Faxonius rusticus)

S24 Identification or verification with genetic analysis of

Common reed (Phragmites australis ssp australis/ Phragmites australis ssp americanus)

S25 Identification or verification with genetic analysis of

Starry stonewort (Nitellopsis obtusa)

S26 qPCR Diagnostic Assay Design and Validation 1

Species the lab not setup to test for – as needed and mutually

S27 Delivery for returned samples plus shipping costs 1

All types

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