D. Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The NINDS Cellular Neurophysiology Section (CNS) requires one (1) lightsheet microscope system to examine the circuits that surround different subpopulations of vulnerable and resilient dopamine neurons in the substantia nigra. A light-sheet microscope would enable examination of the circuits that innervate dopamine neurons on a large scale in whole mouse brains.

2. Background Information and Objective:

Research in the CNS focuses on circuit and cellular properties of dopaminergic neurons located in the midbrain. The CNS is especially interested in the physiology of a subset of dopamine neuron located in the substantia nigra pars compacta (SNc) that undergo selective cell death in Parkinson’s patients, specifically, in understanding how synaptic and intrinsic conductances differ among subpopulations dopamine neurons with the hope that this may provide insight to why SNc dopamine neurons are particularly vulnerable in Parkinson’s disease. Its’ goal is to identify novel physiological and genetic markers in single SNc dopamine neurons that may be useful in defining subpopulations of these neurons in a meaningful way.

3. Generic Name of Product:

Light-sheet microscope

4. Purchase Description:

Contractor shall provide the following components:

-SmartSPIM Base System -ASI Clearing Objective 22x -Refrigerated Circulator -SmartSPIM S&H -SmartSPIM Installation -estimated shipping

5. Salient characteristics The NINDS Cellular Neurophysiology Section requires one (1) light-sheet microscope system that meets or exceeds the following minimum specifications:

• The system must have 488 nm, 561 nm and 639 nm laser lines installed, including a fiber switch with <500ms switching time

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

• The system must include dual illumination arms using custom optics for axially swept light sheet generation.

• The system must include a detection pathway that includes 22x objective, 180 mm tube lens, motorized 6 position filter wheel, triple emission filter set for 488nm, 525nm, and 639nm fluorescence light.

• The system must contain a high QE sCMOS camera (Hamamatsu Fusion) for detection.

• The system must contain XYZ stage for sample movement as well as a joystick for sample movement, stage for detection objective focus, sample holders: 15mm wide (x15), sample chambers (x2), system assembly optomechanics, complete system enclosure

• The system must include a PC workstation, peripherals and acquisition software.

6. Quantity:

One (1) light-sheet microscope system (inclusive of all system components and peripherals), PC workstation (inclusive of all electronics for system control and data acquisition), associated software and hardware, and user manual are required.

7. Delivery Date:

The contractor shall deliver and install the required equipment within six (6) months after receipt of order. Delivery must be FOB Destination and must include inside installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday.