Combined_syn_sol 75N98026Q01236-04985.docx

Hospital and Laboratory Support Division Office of Logistics and Operations (OLAO) Office of Acquisition and Logistics Management (OALM)

Title: Stopped Flow System and associated components

Combined Synopsis Solicitation

Synopsis No. 75N98026Q01236-04985

COMBINED SYNOPSIS / SOLICITATION WORKFORM

Action Code: Combined Synopsis/Solicitation Date: September 17, 2026 Contracting Office Zip Code: 20892 Classification Code: 6640 Contracting Office Address:

6701 Rockledge Drive Bethesda, MD 20817 Subject/Title: Stopped Flow System and associated components Solicitation Number: 75N98026Q01236-04985 Closing Response Date: September 24, 2026 at 10:00 A.M. Eastern Daylight Time (EDT) Questions Due Date: September 19, 2026 at 10:00 A.M Eastern Daylight Time (EDT) Contact Point(s): Luz Casey, Program Specialist/Contract Specialist Contract Award Type: Firm Fixed Price Contract Award: TBD Contract Award Dollar Amount: TBD Contract Line Item Number(s): TBD Contractor Award Date: TBD Contractor Name: TBD Brand Name or Equal Acquisition (FAR 52.211-6): Yes Place of Contract Performance: Research Triangle Park, NC 27709 Set-aside Status: YES Small

Description of Requirement: The NIEHS requires the acquisition of a Stopped Flow System and associated components. The Nucleolar Integrity Group at the National Institute of Environmental Health Sciences (NIEHS) investigates molecular machines involved in critical RNA processing pathways through an integrated approach combining structural, molecular, and cellular biology. The Group's current research focuses primarily on three areas: ribosome assembly, tRNA processing, and viral RNA processing.

A critical requirement of these research programs is the ability to characterize rapid molecular events and determine the kinetics of RNA degradation and multi-protein complex formation associated with ribosome biogenesis and RNA processing. Conventional endpoint assays are insufficient to fully resolve these rapid processes because many biologically important intermediate steps occur on millisecond or faster timescales. The Group therefore requires a high-sensitivity stopped-flow instrument capable of monitoring rapid biochemical reactions in real time.

We require an instrument that is specifically suited for rapid kinetic measurements involving enzyme activity, protein folding, protein-ligand interactions, and other biochemical reactions. Its high sensitivity and rapid acquisition capabilities should be able to allow NIEHS investigators to monitor transient reaction intermediates and characterize the sequence and kinetics of molecular events. A significant requirement for the Group is the ability to collect fluorescence measurements with high sensitivity while also supporting absorbance measurements without requiring substantial system reconfiguration.

Precise temperature control is also required for the Group's kinetic studies. We require a system that can provide controlled experimental temperatures across a broad operating range of approximately -20°C to 85°C, allowing reaction conditions to be maintained consistently from the initiation of the experiment through data acquisition. Temperature control is essential for accurately characterizing reaction rates and determining the effects of temperature on molecular processes. We require that the instrument come with rapid mixing and detection capabilities which are particularly important for the Group's research. The SFM-3000 system can provide valid measurements at reaction times as early as approximately 0.2 milliseconds, enabling investigators to study exceptionally fast biochemical events.

We require a Stopped-Flow System instrument from a vendor that will enable the Nucleolar Integrity Group to:

· Measure the real-time kinetics of RNA degradation and processing reactions.

· Characterize the formation and dissociation of multi-protein and RNA-protein complexes.

· Resolve rapid intermediate steps in ribosome assembly and RNA processing pathways.

· Determine substrate and product binding kinetics.

· Identify rate-limiting steps within biochemical reaction pathways.

· Characterize reaction mechanisms and transient intermediates.

· Measure enzyme kinetics and other rapid biochemical processes with high temporal resolution.

· Conduct fluorescence and absorbance measurements under controlled temperature conditions.

· Minimize sample consumption through the use of low-volume flow cells.

· Install the instrument.

· Provide training on how to operate the instrument and analyze data obtained from the instrument.

· Provide operational and analytical software for use with the instrument.

· Provide the necessary accessories needed for routine use of the instrument in typical experimental protocols.

Acquisition of this instrument is necessary to provide NIEHS investigators with the specialized capability to directly measure rapid molecular events that cannot be adequately characterized using conventional steady-state or endpoint techniques. The system will substantially enhance the Group's ability to define reaction mechanisms, identify kinetic intermediates and rate-limiting steps, and develop a more complete understanding of molecular processes involved in ribosome assembly, tRNA processing, and viral RNA processing. The requested SFM-3000 Stopped-Flow System will therefore provide an essential research capability supporting the NIEHS mission to advance understanding of fundamental biological processes and mechanisms relevant to human health and disease.

The Contractor shall provide all resources necessary to accomplish the tasks and deliverables described in this Statement of Work (SOW). Subject to prior approval of the Contracting Officer (CO), the Contractor may acquire the services of a subcontractor and/or consultants to provide specialized services if deemed appropriate and within scope by the COR.

The Contractor shall employ a technical approach, utilize organizational resources, and implement management controls to achieve the cost, schedule, and performance requirements throughout the execution of the Contract Requirements. The Contractor shall be responsible for providing, managing, and overseeing its personnel, as well as any subcontractor/consultant efforts used in the performance of the Contract Requirements.

This is intended to be a firm-fixed price contract.

Period of Performance: This is a one-time supply acquisition with associated training and warranty support.

Delivery Location:

NIH/NIEHS

105 TW Alexander Drive Bldg. 110, NIEHS Warehouse Research Triangle Park, NC 27709

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued.

The solicitation number is 75N98026Q01236-04985 and the solicitation is issued as a request for quote (RFQ). The solicitation document, the incorporated provisions, and clauses are those in effect through Federal Acquisition Circular 2024-07, effective August 29, 2024. This solicitation is being released unrestricted. The NAICS code is 334516. This requirement is set aside for small business. This acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13. The Statement of Need and any terms and conditions relevant to the requirement are included as attachments. The provisions at FAR Clause 52.212-1, Instructions to Offerors – Commercial Items, applies to this acquisition. The following addendum related to the Instructions to Offerors is included:

Addendum to FAR Clause 52.212-1, Instructions to Offerors – Commercial Items. Please refer to this addendum for additional attachments, including the Statement of Work and other relevant information.

Evaluations will be conducted in accordance with the provision at FAR Clause 52.212-2. Offerors will be evaluated as follows: EVALUATION-COMMERCIAL ITEMS (OCT 2014) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers:

· Technical Proposal/Equipment Capability

· Equipment Warranty and Maintenance Coverage

· Delivery

· Price The Government will make award to the responsible vendor whose quote represents the lowest price technically acceptable, considering equipment capability, equipment warranty and maintenance coverage, delivery and price. The Government may select a higher-priced quote if it is determined that the additional technical merit or lower performance risk warrants the price premium.

Options. The Government will evaluate offers for award purposes by adding the total price for all options to the total price for the basic requirement. The Government may determine that an offer is unacceptable if the option prices are significantly unbalanced. Evaluation of options shall not obligate the Government to exercise the option(s). A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer’s specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award. Please refer to the addendum for additional evaluation information. Each Offeror must include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications – Commercial Items, with its offer.

Please note, each Offeror shall complete only paragraph (b) of this provision if the Offeror has completed the annual representations and certification electronically via the System for Award Management (SAM) website located at https://www.sam.gov/portal.

· FAR Clause 52.212-4, Contract Terms and Conditions – Commercial Items, applies to this acquisition. The terms and conditions outlined in the following addendum also apply:

· Addendum to FAR Clause 52.212-4, Contract Terms and Conditions – Commercial Items. Please refer to this addendum for additional terms and conditions related to the requirement.

· FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items, applies to this acquisition. The full text version of FAR 52.212-5 included in the addenda as Attachment 5 indicate which FAR clauses and provisions are applicable to this acquisition.

Evaluation of Options Provision, The Government will evaluate offers for award purposes by adding the total price for all options to the total price for the basic award. This solicitation notified offerors that the award will include the Government's unilateral option to extend performance for an additional period up to six months under FAR 52.217-8, during which the pricing and terms of the period in which the option was exercised would apply. The Government cannot predict if or when the option may be exercised. Because any exercise of the Government's option extends the pricing and terms of the period in which the option was exercised, the Government expressly and affirmatively evaluates pricing for the option to extend under 52.217-8 co-extensive with the Government's price evaluation for each of the base and option periods of this award. Because pricing for each period subject to possible extension under the 52.217-8 has been evaluated, pricing for any possible future use of that option to extend has, likewise, been evaluated and would apply in strict accordance with this evaluation in the event of the Government’s exercise of the option to extend services. (End of Provision)

Submission Requirements: Responses must include sufficient information to demonstrate the respondent’s capability to provide the required product or service. Price quotations shall include, at a minimum: unit price, list price, shipping and handling costs, delivery timeframe after award, delivery terms, prompt payment discount terms, F.O.B. point (destination or origin), product or catalog numbers, product descriptions, and any other relevant information.

Evaluation factors may include, but are not limited to: equipment capability, equipment warranty and maintenance coverage, delivery, past performance, technical capability, special features, trade-in considerations, expected lifecycle, warranty, maintenance availability, and environmental or energy efficiency considerations.

Respondents claiming to be manufacturers or authorized resellers of a specified brand-name product must provide:

(a) Product, catalog, model, and/or part number(s);

(b) Product description;

(c) Documentation demonstrating that the offered item meets all required salient characteristics;

(d) Quantity;

(e) Estimated price or cost;

(f) Shipping, handling, and/or installation charges; and

(g) Delivery timeframe after receipt of order.

Responses must also include the Unique Entity Identifier (UEI), Taxpayer Identification Number (TIN), and business size certification. All offerors must be actively registered in the System for Award Management (SAM) at www.sam.gov.

All responses must be received by September 24, 2026 and must reference solicitation number 75N98026Q01236-04985. Responses may be submitted electronically to Luz.Casey@nih.gov.

Contact Information:

Contract Specialist/Program Specialist: Luz Casey Email: Luz.Casey@nih.gov image1.png