C04_Attachment II - Statement of Work_1232SA26Q1062_Redacted.pdf

STATEMENT OF WORK (SOW)

Project Title: Procurement of Cryostat Microtome for Sectioning Snap-Frozen Animal Tissues Purpose: To support histological and molecular pathology workflows in prion disease research using precision cryosectioning of frozen tissues

1.0 Scope of Work:

This project seeks to acquire a cryostat microtome for sectioning snap-frozen mouse and large animal tissues. The system will support downstream histopathological, immunohistochemical (IHC) which is the gold standard for diagnostic evaluation of prion disease. Frozen sections allow for staining that is not always available in traditional paraffin embedded tissues. The goal is to increase efficiency, precision, and biosafety in frozen tissue preparation workflows.

2.0 Background:

Our research program routinely analyzes brain, lymphoid, and other tissues from prion-infected mice, cervids, and livestock. These tissues are snap-frozen in liquid nitrogen or dry ice/isopentane to preserve protein conformation and nucleic acids for subsequent histology, immunodetection (e.g., PrPSc deposition), and molecular assays. Cryostat-based tissue sectioning is essential for producing thin, reproducible sections from these frozen samples.

Currently, our lab does not own a dedicated cryostat system. Although the campus has a cryostat, due to the shared use of the Microscopy Service’s cryostat, it is unable to be properly deconned after TSE exposure. We are unable to use that cryostat and must purchase our own.

To support timely and consistent sample processing, we propose the purchase of a modern, research-grade cryostat with built-in safety features, temperature control, and user ergonomics.

3.0 Technical Requirements / Tasks:

The cryostat system must meet the following minimum specifications:

• Temperature-controlled cryochamber with adjustable range (e.g., −10°C to −35°C)

• Precision microtome integrated within cryochamber

• Compatible with mouse, deer, and livestock tissue block sizes

• Section thickness range: 1–100 microns

• Automatic or semi-automatic cutting functionality

• Anti-roll plate and specimen orientation system

• Stainless steel or antimicrobial chamber design for biosafety

• UV decontamination or formaldehyde fumigation-compatible (preferred)

• Safety features including finger guard, specimen retraction, and locking mechanisms

• LED illumination and ergonomic design for prolonged use

• Optional: Cryobar for quick freezing samples

• Optional: Disposable blade holder system

4.0 Government Furnished:

Access to the facility will be coordinated for equipment delivery, installation, and training. No other equipment or infrastructure will be provided by the government.

5.0 Deliverables / Schedule:

• Equipment must be delivered and installed within 45–60 days of contract award

• On-site or remote training must be provided

• User manual, validation documents, and warranty documentation included

6.0 Evaluation Factors:

• Technical compliance with specifications

• Equipment reliability and ease of use

• Warranty length and service/support availability

• Delivery schedule

• Biosafety and decontamination options

7.0 Technical Point of Contact:

8.0 Place of Delivery:

National Centers for Animal Health

Nation Animal Disease Center

1920 Dayton Ave, Ames, IA 50010