Book1.xlsx

This file is a pricing and supply list for customized molecular biology reagents required for viral diagnostics. The U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is soliciting primers and probes for real-time PCR assays targeting multiple pathogens including Dengue virus (four serotypes), Chikungunya virus, Zika virus, Mayaro virus, Hantavirus, Orthobunyavirus variants, Influenza A and B viruses with specific subtypes (H3, H1pdm/SwH1N1, H1 seasonal), and SARS-CoV-2. The requested materials will be used for molecular diagnosis with serum, saliva, blood, urine, pharyngeal swabs, rectal swabs, fecal samples, and egg culture samples from febrile and respiratory surveillance protocols.

The supply list details specific oligonucleotide products with quantities ranging from 1 to 370 units, including custom oligonucleotides, dual-labeled probes with various fluorophore and quencher combinations, primers in different purification grades, and pre-designed multiplex primer and probe sets for influenza and SARS-CoV-2 detection. All assays have been validated using TaqMan 7500/7500 Fast Real-time PCR systems and QuantStudio platforms. The materials specified must remain identical to those documented in existing standard operating procedures (SOPs) to avoid requiring complete redesign and revalidation, which would delay pathogen detection critical to public health surveillance. Delivery is required to NAMRU SOUTH at 3230 Lima Place, Washington, DC 20521. The solicitation is being conducted by the Department of the Navy Naval Supply Systems Command.