B08_Appendix_D_-_Methods_with_Reporting_Limits.xlsx
XLSX spreadsheet 31 KB Posted
- Attached to
- Harmful Algal Blooms (HABs) Analytical Services
- Solicitation number
- 140G0225R0001
About this file
This document appears to be an appendix detailing the analytical methods and reporting limits for a federal contract opportunity related to Harmful Algal Blooms (HABs) analytical services.
The key details include the required analytical methods for various matrices (freshwater, sediment, animal tissue, SPATT) and analytes (cyanotoxins, chlorophyll, taste/odor compounds, plankton enumeration). The methods specified include HPLC, LC-MS/MS, ELISA, fluorometry, and microscopy. The document outlines the required method details, reporting limits, and quality control requirements.
This appendix is likely part of the solicitation for the "Harmful Algal Blooms (HABs) Analytical Services" contract opportunity, issued by the Department of the Interior US Geological Survey Office of Acquisitions and Grants. The solicitation number is 140G0225R0001.
View the file
Other files
| File | Type | Posted |
|---|---|---|
| Sol_140G0225R0001_Amd_0001.pdf | ||
| Sol_140G0225R0001.pdf | ||
| B08_Appendix_B_-_Example_of_EDD.txt | TXT text file | |
| B08_Appendix_C_-_Daily_Receipt_File_Example.xlsx | XLSX spreadsheet | |
| B08_Appendix_E_-_ASR.docx | DOCX document | |
| B08_Appendix_A_-_Pricing_Schedule.xlsx | XLSX spreadsheet |
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Text version
HABs
| Line Item | Parameter | Sample Matrix | Analytical Method Description | Comments | Concensus Method number(s) | Contract Laboratory Method number(s) | Contract Laboratory Method Name | Contract Laboratory Capability: Published Method Modification Y/N | MDL Study Date Completion | MDL Concentration | MDL Units | Reporting Limit | Reporting Limit Units | Date of Acceptable PT | PT Provider | Comments for any failed analyte | Corrective Action Estimated Date of Completion | Date of PT Retake with acceptable results | Number of Instruments available | Number of Trained Analysts available | Maximum Capacity (Analyses / week) | Turn-Around-Time (Days) | If subcontracting labs performing the test - Copy and repeat columns to the right per per subcontractor. | Subcontractor A - Date of last Acceptable PT2 | Subcontractor A - PT Provider2 | Subcontractor A- Capacity (per week) | Subcontractor A-TAT |
| 1 | Cyanotoxin panel | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 2 | Cyanotoxin panel | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 3 | Cyanotoxin panel | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 4 | Cyanotoxin panel | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 5 | Cyanotoxin panel | Freshwater | ELISA | Custom or modified | |||||||||||||||||||||||
| 6 | Microcystins | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | EPA 544 | ||||||||||||||||||||||
| 7 | Microcystins | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 8 | Microcystins | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 9 | Microcystins | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 10 | Microcystins | Freshwater | ELISA | EPA 546 | |||||||||||||||||||||||
| 11 | Nodularin | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | EPA 544 | ||||||||||||||||||||||
| 12 | Nodularin | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 13 | Nodularin | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 14 | Nodularin | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 15 | Nodularin | Freshwater | ELISA | EPA 546 | |||||||||||||||||||||||
| 16 | Cylindrospermopsin | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | EPA 545 | ||||||||||||||||||||||
| 17 | Cylindrospermopsin | Marine Water | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 18 | Cylindrospermopsin | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 19 | Cylindrospermopsin | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 20 | Cylindrospermopsin | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 21 | Cylindrospermopsin | Freshwater | ELISA | Custom or modified | |||||||||||||||||||||||
| 22 | Anatoxin-a | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | EPA 545 | ||||||||||||||||||||||
| 23 | Anatoxin-a | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 24 | Anatoxin-a | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 25 | Anatoxin-a | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 26 | Anatoxin-a | Freshwater | ELISA | Custom or modified | |||||||||||||||||||||||
| 27 | Saxitoxin | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 28 | Saxitoxin | Marine Water | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 29 | Saxitoxin | Sediment | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 30 | Saxitoxin | Animal Tissue | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 31 | Saxitoxin | SPATT | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 32 | Saxitoxin | Freshwater | ELISA | Custom or modified | |||||||||||||||||||||||
| 33 | Saxitoxin | Marine Water | ELISA | Custom or modified | |||||||||||||||||||||||
| 34 | Guanitoxin | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 35 | Guanitoxin | Freshwater | ELISA | Custom or modified | |||||||||||||||||||||||
| 36 | Additional & Unknown cyanotoxin and congener identification / quantification | Freshwater | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 37 | Additional & Unknown cyanotoxin and congener identification / quantification | Marine Water | HPLC or LC-MS/MS | MS: Provide precursor ions and at least 1-2 fragment ions | Custom or modified | ||||||||||||||||||||||
| 38 | MIB & Geosmin | Freshwater | SPME GC-MS | Taste and odor compounds | SM 6040D | ||||||||||||||||||||||
| 39 | MIB & Geosmin | Marine Water | SPME GC-MS | Taste and odor compounds | SM 6040D | ||||||||||||||||||||||
| 40 | Chlorophyll-a, corrected for pheophytin | Surface Water | Fluorescence, acetone extraction, HCl acidification | Chlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three. |
Specify extraction time.
| NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification". | EPA 445 acidification | |||||
| 41 | Pheophytin-a | Surface Water | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 42 | Chlorophyll-a, uncorrected for pheophytin | Surface Water | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 43 | Chlorophyll-a, corrected for pheophytin | Marine Water | Fluorescence, acetone extraction, HCl acidification | Chlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three. |
Specify extraction time.
| NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification". | EPA 445 acidification | |||||
| 44 | Pheophytin-a | Marine Water | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 45 | Chlorophyll-a, uncorrected for pheophytin | Marine Water | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 46 | Chlorophyll-a, corrected for pheophytin | Periphyton | Fluorescence, acetone extraction, HCl acidification | Chlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three. |
Specify extraction time.
| NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification". | EPA 445 acidification | |||||
| 47 | Pheophytin-a | Periphyton | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 48 | Chlorophyll-a, uncorrected for pheophytin | Periphyton | Fluorescence, acetone extraction, HCl acidification | EPA 445 acidification | ||
| 49 | Chlorophyll-a | Surface Water | Spectrophotometric | Report Chlorophyll a, b, and c. | ||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 B | |||||
| 50 | Chlorophyll-b | Surface Water | Spectrophotometric | APHA 10150 B | ||
| 51 | Chlorophyll-c | Surface Water | Spectrophotometric | APHA 10150 B | ||
| 52 | Pheophytin-a | Surface Water | Spectrophotometric | APHA 10150 B | ||
| 53 | Chlorophyll-a | Marine Water | Spectrophotometric | Report Chlorophyll a, b, and c. | ||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 B | |||||
| 54 | Chlorophyll-b | Marine Water | Spectrophotometric | APHA 10150 B | ||
| 55 | Chlorophyll-c | Marine Water | Spectrophotometric | APHA 10150 B | ||
| 56 | Pheophytin-a | Marine Water | Spectrophotometric | APHA 10150 B | ||
| 57 | Chlorophyll-a | Periphyton | Spectrophotometric | Report Chlorophyll a, b, and c. | ||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 B | |||||
| 58 | Chlorophyll-b | Periphyton | Spectrophotometric | APHA 10150 B | ||
| 59 | Chlorophyll-c | Periphyton | Spectrophotometric | APHA 10150 B | ||
| 60 | Pheophytin-a | Periphyton | Spectrophotometric | APHA 10150 B | ||
| 61 | Chlorophyll-a | Surface Water | Fluorometric | |||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 C | |||||
| 62 | Pheophytin-a | Surface Water | Fluorometric | APHA 10150 C | ||
| 63 | Chlorophyll-a | Marine Water | Fluorometric | |||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 C | |||||
| 64 | Pheophytin-a | Marine Water | Fluorometric | APHA 10150 C | ||
| 65 | Chlorophyll-a | Periphyton | Fluorometric | |||
| Specify acid, extraction solvent, and detection method used. | APHA 10150 C | |||||
| 66 | Pheophytin-a | Periphyton | Fluorometric | APHA 10150 C | ||
| 67 | Chlorophylls and degradation products | Surface Water | HPLC | APHA 10150 D | ||
| 68 | Chlorophylls and degradation products | Marine Water | HPLC | APHA 10150 D | ||
| 69 | Chlorophylls and degradation products | Periphyton | HPLC | APHA 10150 D | ||
| 70 | Algal chlorophyll and cartenoid pigments | Surface Water | HPLC | APHA 10150 E | ||
| 71 | Algal chlorophyll and cartenoid pigments | Marine Water | HPLC | APHA 10150 E | ||
| 72 | Algal chlorophyll and cartenoid pigments | Periphyton | HPLC | APHA 10150 E | ||
| 73 | Chlorophyll-a | Surface Water | Narrow-band fluorimeter | |||
| Specify fluorimeter bandpass and extraction solvent used. | EPA 445 narrow band modification | |||||
| 74 | Chlorophyll-a | Marine Water | Narrow-band fluorimeter | |||
| Specify fluorimeter bandpass and extraction solvent used. | EPA 445 narrow band modification | |||||
| 75 | Chlorophyll-a | Periphyton | Narrow-band fluorimeter | |||
| Specify fluorimeter bandpass and extraction solvent used. | EPA 445 narrow band modification | |||||
| 76 | Phycocyanin | Surface Water | Fluorometric | Specify fluorimeter bandpass, acid and extraction solvent used. | Custom or modified | |
| 77 | Phycocyanin | Marine Water | Fluorometric | Specify fluorimeter bandpass, acid and extraction solvent used. | Custom or modified | |
| 78 | Phycocyanin | Periphyton | Fluorometric | Specify fluorimeter bandpass, acid and extraction solvent used. | Custom or modified | |
| 79 | Phycoerythrin | Surface Water | Fluorometric | Specify fluorimeter bandpass, extraction solvent, and purification used. | Custom or modified | |
| 80 | Phycoerythrin | Marine Water | Fluorometric | Specify fluorimeter bandpass, extraction solvent, and purification used. | Custom or modified | |
| 81 | Phycoerythrin | Periphyton | Fluorometric | Specify fluorimeter bandpass, extraction solvent, and purification used. | Custom or modified | |
| 82 | Plankton enumeration (phyto plankton, picoplankton) | Water | Microscope | Specify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option. | Custom or modified | |
| 83 | Plankton enumeration (phyto plankton, picoplankton) | Periphyton | Microscope | Specify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option. | Custom or modified | |
| 84 | Plankton enumeration (phyto plankton, picoplankton) | Water | Imaging flow cytometry | Indicate level of automation for detection and image analysis. | Custom or modified | |
| 85 | Picocyanobacteria enumeration | Water | Microscope | Specify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option. | Custom or modified | |
| 86 | Picocyanobacteria enumeration | Periphyton | Microscope | Specify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option. | Custom or modified | |
| 87 | Picocyanobacteria enumeration | Water | Imaging flow cytometry | Indicate level of automation for detection and image analysis. | Custom or modified | |
| 88 | Moisture Content | Solid samples | Gravimetric | Required if results are requested on a dry-weight basis. | D2216-19 |
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