B08_Appendix_A_-_Pricing_Schedule.xlsx

XLSX spreadsheet 51 KB Posted

Attached to
Harmful Algal Blooms (HABs) Analytical Services Federal contract opportunity
Solicitation number
140G0225R0001
Issued by
Department of the Interior US Geological Survey Office of Acquisitions and Grants

About this file

This document appears to be a pricing schedule appendix for a federal contract opportunity related to Harmful Algal Blooms (HABs) Analytical Services. The contract is solicited by the Department of the Interior US Geological Survey Office of Acquisitions and Grants.

The pricing schedule outlines various analytical tests and methods for cyanotoxins, chlorophyll, phytoplankton, and other parameters across different sample matrices such as freshwater, marine water, sediment, and animal tissue. It provides estimated annual quantities and unit pricing for each test over a 5-year base period with a 6-month extension. The required analytical methods and performance specifications are specified for each test. The contract appears to be seeking a broad range of HABs-related analytical services to support environmental monitoring and research programs.

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Other files attached to Harmful Algal Blooms (HABs) Analytical Services, newest first.
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Sol_140G0225R0001_Amd_0001.pdf PDF
Sol_140G0225R0001.pdf PDF
B08_Appendix_B_-_Example_of_EDD.txt TXT text file
B08_Appendix_C_-_Daily_Receipt_File_Example.xlsx XLSX spreadsheet
B08_Appendix_E_-_ASR.docx DOCX document
B08_Appendix_D_-_Methods_with_Reporting_Limits.xlsx XLSX spreadsheet

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HABs

Line ItemParameterSample MatrixAnalytical Method DescriptionCommentsConcensus Method number(s)Contract Laboratory Method number(s)Contract Laboratory Method NameEST. Annual QUANTITYUnit Price Year 1Estimated Total Year 1Estimated Annual Quantities Year 2Unit Price
Year 2Estimated Total Year 2Estimated Annual Quantities Year 3Unit Price
Year 3Estimated Total Year 3Estimated Annual Quantities Year 4Unit Price
Year 4Estimated Total Year 4Estimated Annual Quantities Year 5Unit Price
Year 5Estimated Total Year 5Estimated Quantities 6 month Extention (Qty = hafl of the annual est only)Unit Price
6 month ExtentionEstimated Total 6-month extention
1Cyanotoxin panelFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified707070707035
2Cyanotoxin panelSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
3Cyanotoxin panelAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
4Cyanotoxin panelSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified505050505025
5Cyanotoxin panelFreshwaterELISACustom or modified200200200200200100
6MicrocystinsFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsEPA 544707070707035
7MicrocystinsSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
8MicrocystinsAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
9MicrocystinsSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
10MicrocystinsFreshwaterELISAEPA 546555553
11NodularinFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsEPA 544606060606030
12NodularinSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
13NodularinAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
14NodularinSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
15NodularinFreshwaterELISAEPA 546555553
16CylindrospermopsinFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsEPA 545606060606030
17CylindrospermopsinMarine WaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
18CylindrospermopsinSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
19CylindrospermopsinAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
20CylindrospermopsinSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
21CylindrospermopsinFreshwaterELISACustom or modified555553
22Anatoxin-aFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsEPA 545606060606030
23Anatoxin-aSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
24Anatoxin-aAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
25Anatoxin-aSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
26Anatoxin-aFreshwaterELISACustom or modified555553
27SaxitoxinFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified606060606030
28SaxitoxinMarine WaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
29SaxitoxinSedimentHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified202020202010
30SaxitoxinAnimal TissueHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified10101010105
31SaxitoxinSPATTHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
32SaxitoxinFreshwaterELISACustom or modified555553
33SaxitoxinMarine WaterELISACustom or modified555553
34GuanitoxinFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified303030303015
35GuanitoxinFreshwaterELISACustom or modified555553
36Additional & Unknown cyanotoxin and congener identification / quantificationFreshwaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified555553
37Additional & Unknown cyanotoxin and congener identification / quantificationMarine WaterHPLC or LC-MS/MSMS: Provide precursor ions and at least 1-2 fragment ionsCustom or modified555553
38MIB & GeosminFreshwaterSPME GC-MSTaste and odor compoundsSM 6040D555553
39MIB & GeosminMarine WaterSPME GC-MSTaste and odor compoundsSM 6040D111111
40Chlorophyll-a, corrected for pheophytinSurface WaterFluorescence, acetone extraction, HCl acidificationChlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three.

Specify extraction time.

NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification".EPA 445 acidification555553
41Pheophytin-aSurface WaterFluorescence, acetone extraction, HCl acidificationEPA 445 acidification555553
42Chlorophyll-a, uncorrected for pheophytinSurface WaterFluorescence, acetone extraction, HCl acidificationEPA 445 acidification555553
43Chlorophyll-a, corrected for pheophytinMarine WaterFluorescence, acetone extraction, HCl acidificationChlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three.

Specify extraction time.

NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification".EPA 445 acidification222221
44Pheophytin-aMarine WaterFluorescence, acetone extraction, HCl acidificationEPA 445 acidification222221
45Chlorophyll-a, uncorrected for pheophytinMarine WaterFluorescence, acetone extraction, HCl acidificationEPA 445 acidification222221
46Chlorophyll-a, corrected for pheophytinPeriphytonFluorescence, acetone extraction, HCl acidificationChlorophyll-a corrected = Chlorophyll-a uncorrected - Pheophytin-a. Report all three.

Specify extraction time.

NOTE: EPA 445 is preferred over APHA 10150 series. Denote as "EPA 445 acidification".EPA 445 acidification222221
47Pheophytin-aPeriphytonFluorescence, acetone extraction, HCl acidificationEPA 445 acidification222221
48Chlorophyll-a, uncorrected for pheophytinPeriphytonFluorescence, acetone extraction, HCl acidificationEPA 445 acidification222221
49Chlorophyll-aSurface WaterSpectrophotometricReport Chlorophyll a, b, and c.
Specify acid, extraction solvent, and detection method used.APHA 10150 B555553
50Chlorophyll-bSurface WaterSpectrophotometricAPHA 10150 B555553
51Chlorophyll-cSurface WaterSpectrophotometricAPHA 10150 B555553
52Pheophytin-aSurface WaterSpectrophotometricAPHA 10150 B555553
53Chlorophyll-aMarine WaterSpectrophotometricReport Chlorophyll a, b, and c.
Specify acid, extraction solvent, and detection method used.APHA 10150 B555553
54Chlorophyll-bMarine WaterSpectrophotometricAPHA 10150 B555553
55Chlorophyll-cMarine WaterSpectrophotometricAPHA 10150 B555553
56Pheophytin-aMarine WaterSpectrophotometricAPHA 10150 B555553
57Chlorophyll-aPeriphytonSpectrophotometricReport Chlorophyll a, b, and c.
Specify acid, extraction solvent, and detection method used.APHA 10150 B555553
58Chlorophyll-bPeriphytonSpectrophotometricAPHA 10150 B555553
59Chlorophyll-cPeriphytonSpectrophotometricAPHA 10150 B555553
60Pheophytin-aPeriphytonSpectrophotometricAPHA 10150 B555553
61Chlorophyll-aSurface WaterFluorometric
Specify acid, extraction solvent, and detection method used.APHA 10150 C555553
62Pheophytin-aSurface WaterFluorometricAPHA 10150 C555553
63Chlorophyll-aMarine WaterFluorometric
Specify acid, extraction solvent, and detection method used.APHA 10150 C555553
64Pheophytin-aMarine WaterFluorometricAPHA 10150 C555553
65Chlorophyll-aPeriphytonFluorometric
Specify acid, extraction solvent, and detection method used.APHA 10150 C555553
66Pheophytin-aPeriphytonFluorometricAPHA 10150 C555553
67Chlorophylls and degradation productsSurface WaterHPLCAPHA 10150 D555553
68Chlorophylls and degradation productsMarine WaterHPLCAPHA 10150 D555553
69Chlorophylls and degradation productsPeriphytonHPLCAPHA 10150 D555553
70Algal chlorophyll and cartenoid pigmentsSurface WaterHPLCAPHA 10150 E555553
71Algal chlorophyll and cartenoid pigmentsMarine WaterHPLCAPHA 10150 E555553
72Algal chlorophyll and cartenoid pigmentsPeriphytonHPLCAPHA 10150 E555553
73Chlorophyll-aSurface WaterNarrow-band fluorimeter
Specify fluorimeter bandpass and extraction solvent used.EPA 445 narrow band modification202020202010
74Chlorophyll-aMarine WaterNarrow-band fluorimeter
Specify fluorimeter bandpass and extraction solvent used.EPA 445 narrow band modification10101010105
75Chlorophyll-aPeriphytonNarrow-band fluorimeter
Specify fluorimeter bandpass and extraction solvent used.EPA 445 narrow band modification10101010105
76PhycocyaninSurface WaterFluorometricSpecify fluorimeter bandpass, acid and extraction solvent used.Custom or modified200200200200200100
77PhycocyaninMarine WaterFluorometricSpecify fluorimeter bandpass, acid and extraction solvent used.Custom or modified505050505025
78PhycocyaninPeriphytonFluorometricSpecify fluorimeter bandpass, acid and extraction solvent used.Custom or modified505050505025
79PhycoerythrinSurface WaterFluorometricSpecify fluorimeter bandpass, extraction solvent, and purification used.Custom or modified10101010105
80PhycoerythrinMarine WaterFluorometricSpecify fluorimeter bandpass, extraction solvent, and purification used.Custom or modified10101010105
81PhycoerythrinPeriphytonFluorometricSpecify fluorimeter bandpass, extraction solvent, and purification used.Custom or modified10101010105
82Plankton enumeration (phytoplankton, picoplankton)WaterMicroscopeSpecify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option.Custom or modified10010010010010050
83Plankton enumeration (phytoplankton, picoplankton)PeriphytonMicroscopeSpecify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option.Custom or modified10010010010010050
84Plankton enumeration (phytoplankton, picoplankton)WaterImaging flow cytometryIndicate level of automation for detection and image analysis.Custom or modified10010010010010050
85Picocyanobacteria enumerationWaterMicroscopeSpecify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option.Custom or modified505050505025
86Picocyanobacteria enumerationPeriphytonMicroscopeSpecify counting technique and magnification, reference taxonomy standard. Provide voucher for analysis as add-on option. Provide slide preservation as add-on option.Custom or modified505050505025
87Picocyanobacteria enumerationWaterImaging flow cytometryIndicate level of automation for detection and image analysis.Custom or modified10010010010010050
88Moisture ContentSolid samplesGravimetricRequired if results are requested on a dry-weight basis.D2216-19202020202010

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