B. AP Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this acquisition is to obtain iPSC derived Dopaminergic and Glutametrgic Neuronal cells, which is essential for the 3D invitro neuronal model for drug screening.

2. Background Information and Objective:

The National Center for Advancing Translational Sciences (NCATS) is a part of the NIH, with a mission to catalyze the generation of innovative methods and technologies that will enhance the development, testing, and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions. A significant emphasis of NCATS is the rapid development of effective therapies for patients diagnosed with these conditions. NCATS is directly addressing this problem by discovering new technologies and other approaches that could significantly accelerate the process of developing and deploying solutions that all translational researchers can use. iCell Dopa and Gluta Neurons are iPS cell-derived human midbrain floorplate dopaminergic and glutamatergic neurons respectively, differentiated in protocols licensed and adapted from the Lorenz Studer lab (Memorial Sloan Kettering) and industrialized for scale at FCDI. They are fully differentiated, >70% pure midbrain neurons, expressed relevant neuronal markers. This neurons are appropriate for electro physiological testing invitro also compatible to wide range of biochemical and cell-based assays like cell viability, cell mitophagy, calcium signalling, neurites outgrowth. The objective of this procurements is to use these iPSC derived human mature neurons in 3D invitro circuitry model to get invivo relevance interims of morphology, network formation and mainly functionality in pharmacological assays. 3D Tissue Bio printing lab using these cells for past 5 years and getting scientif ically satisfactory results which has been published in multiple peered reviewed journals.

The project is ongoing and to achieve consistency in the observation and model designing the vendor, Fujif ilm is the only option with respect to the continuity of science.

3. Generic Name of Product:

iCell DopaNeurons and iCell GlutaNeurons

4. Purchase Description:

These iPSC-derived Neuronal Cells:

3 each iCell GlutaNeurons Kit, 01279, #R1034 ≥6.0 M 3 each iCellGABANeurons Kit, 01279, #R1011 ≥ 4.0 M 3 each iCell DopaNeurons Kit, 01279, #R1032 ≥5.0 M 42 each iCell Astrocytes Kit, 01434, #R1092 ≥1.0 M 24 each iCell Motor Neurons Kit, 01279, #R1049 ≥3.0 M

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

14 each iCell Nervous System Supplement, #M1031, 1mL 15 each iCell Astrocytes Kit, 01434, #R1092 ≥1.0 M 10 each iCell DopaNeurons, 01279, [CELL ONLY] #C1028 ≥5.0 M 5 each iCell GABANeurons, 01279, [CELL ONLY] #C1008 ≥ 4.0 M 4 each MyCell DopaNeurons, 01279, [CELL ONLY] #C1113 ≥5.0 M

5. Salient characteristics These iPSC neurons have been used in ongoing projects to develop 3D neuronal circuitry models and various functional assays. Protocol has been established with pharmacological modulations on these models which are invivo relevant.

6. Quantity:

See Purchase Description

7. Delivery Date:

5-10 days ARO

8. Period of Performance:

One-time delivery

9. Option(s):

N/A