Attachment1_Specifications_1232SA26Q1239.pdf
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- Attached to
- 4K Wheat SNP Targeted Amplicon Primer Pool Federal contract opportunity
- Solicitation number
- 1232SA26Q1239
About this file
This is a Specifications document for a federal procurement by the USDA-ARS for a custom wheat genomics product. The Government is procuring one Custom 4K Wheat SNP Targeted Amplicon Primer Pool in a quantity of 15,000 reactions. The primer pool must be the existing Eurofins BioDiagnostics-developed product previously used in a small test run, with this procurement representing a larger quantity of the same formulation. Delivery is required within 60 days after contract award to USDA-ARS, Clark Hall 287, 410 SE Dairy Road, Pullman, Washington 99164, Attention: Travis Ruff.
The primer pool must target approximately 4,000 SNPs within the wheat genome as specified by the Small Grain Genotyping Laboratories and maintain the existing Eurofins-developed multiplex assay structure. Key technical requirements include compatibility with Illumina next-generation sequencing targeted amplicon workflows, support for increased sequence coverage and read depth per marker and sample compared to prior Ion Torrent-based AgriSeq workflows, genotype reporting tied to SNP marker identities and genomic positions, and suitability for hexaploid bread wheat containing A, B, and D subgenomes with extensive sequence homology. The supplier must ensure the delivered primer pool is consistent with the Eurofins design used in the small test run to maintain data continuity and avoid redesign or revalidation. Award is contingent upon satisfactory technical review and acceptance of pending small-scale test-plate data demonstrating acceptable performance, including sufficient sequence coverage and usable genotype call quality, with the Government retaining the option to revise or cancel the requirement prior to award if performance is inadequate.
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| File | Type | Posted |
|---|---|---|
| C04_Solicitation Terms and Conditions_1232SA26Q1239.pdf |
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SPECIFICATIONS
1.0 Specifications Description:
Custom 4K Wheat SNP Targeted Amplicon Primer Pool
Quantity: 1
Unit Type: Primer Pool (15,000 rxn)
Minimum Requirements:
The required item is the existing Eurofins-developed custom ~4,000-marker wheat SNP targeted amplicon primer pool. The primer pool must be the same custom primer pool previously developed by Eurofins BioDiagnostics and used in a small test run. The current procurement is for the same primer pool in a larger quantity.
Minimum requirements:
• Custom SNP primer pool must target approximately 4,000 SNPs within the wheat genome as determined by the Small Grain Genotyping Laboratories (SGGLs).
• Primer pool must maintain the existing Eurofins-developed multiplex assay structure.
• Primer pool must be compatible with an Illumina next-generation sequencing targeted amplicon workflow.
• Primer pool must support increased sequence coverage/read depth per marker and per sample compared with the prior Ion Torrent-based AgriSeq workflow.
• Primer pool must support genotype reporting tied to the intended SNP marker identities and genomic positions.
• Primer pool must be suitable for use in hexaploid bread wheat, which has A, B, and D sub-genomes with extensive homoeologous and paralogous sequence similarity.
• The supplied primer pool must be consistent with the Eurofins-developed design used for the small test run to maintain data continuity and avoid redesign/revalidation.
2.0 Place of Delivery
USDA-ARS
410 SE Dairy Rd.
Clark Hall 287 Pullman, WA 99164 Attn: Travis RuƯ
3.0 Delivery Schedule
Item QTY Unit Place of Delivery
Delivery Date
4K GBS Panel Development/Creation 1 Primer Pool (15,000 rxn)
410 SE. Dairy Rd., Clark Hall 287, Pullman, WA 99164
60 days after award
4.0 Additional Requirements
Award is contingent upon satisfactory technical review and acceptance of the pending small-scale test-plate data generated using the same Eurofins-developed custom 4K wheat SNP targeted amplicon primer pool. If the test-plate data do not demonstrate acceptable performance for the intended wheat SNP genotyping application, including suƯicient sequence coverage/read depth per marker and per sample and usable genotype call quality, the Government may elect not to proceed with award or may revise/cancel the requirement prior to award.
File details come from the government source that posted it. Updated .