Attachment A PWS Whole Genome Sequencing beta.SAM.pdf

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Attached to
Whole Genome Sequencing, Lubbock, TX Federal contract opportunity
Solicitation number
12805B20Q0143
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Plains Area

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Attachment B Wage Determination (Lubbock County) 2015-5243.pdf PDF
Sol_12805B20Q0143.pdf PDF

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Identification of Agency and Activity:

The agency requesting the sorghum sequencing is the USDA-ARS Cropping Systems Research Laboratory located at 3810 4th St, Lubbock, TX 79415. The address where the sequencing data will be utilized at is USDA-ARS Cropping Systems Research Laboratory located at 3810 4th St, Lubbock, TX 79415.

Description of Action:

The Plant Stress and Germplasm Development Unit evaluates crop diversity via bioinformatics of genomic DNA sequence data. We have isolated >300 dwarf mutants. We will use whole genome resequencing to identify the dwarf genes in order to incorporate the optimum dwarf mutations into sorghum inbreed lines and hybrids. This data is essential to the research of four scientists within the Unit and is shared among all employees. Furthermore, the data will be used construct a sorghum breeding database, which will be used agency-wide to accelerate sorghum breeding.

Description of Supplies/Services:

The purchase is for the whole genome sequencing of 300 sorghum (Sorghum bicolor, genome size ~750 Mb) dwarf mutants. We will provide whole genome DNA. Service requested includes construction of the sequencing library and delivery of raw sequencing data. The provider will construct a >500 bp randomly sheared Paired End Library. The desired output is 10 Gb high quality sequencing data per sample. The deliverable would be Raw Sequence Data via Hard Drives within 10 weeks of receiving the samples.

Performance Work Statement Whole Genome Sequencing

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