Attachment 6 SDSs.pdf

G-Biosciences, St Louis, MO, USA ♦ 1-800-628-7730 ♦ 1-314-991-6034 ♦ technical@GBiosciences.com

A Geno Technology, Inc. (USA) brand name

Safety Data Sheet think proteins! think G-Biosciences! www.GBiosciences.com

Bicinchoninic Acid (BCA) Protein Assay

Cat.# 786-570 Part # Description

030B BCA Solution (BCA Reagent A)

224B-B BSA (2mg/mL) Solution

274C Copper Solution (BCA Reagent B)

Cat.# 786-571 Part # Description

030B BCA Solution (BCA Reagent A)

224B-B BSA (2mg/mL) Solution

274C Copper Solution (BCA Reagent B)

Last saved: 7/30/2015 TN mailto:technical@GBiosciences.com

BCA Solution according to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations

Date of issue: 07/29/2015 Revision date: 08/06/2015 Version: 2.1

08/06/2015 EN (English US) Page 1

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1. Identification

Product form : Mixtures

Product name. : BCA Solution

Product code : 030B, 030B-B, 030B-C, 030B-D, 786-844, 786-845, 786-846, 786-847

1.2. Relevant identified uses of the substance or mixture and uses advised against

No additional information available

1.3. Details of the supplier of the safety data sheet

Geno Technology, Inc./ G-Biosciences 9800 Page Avenue Saint Louis, 63132-1429 - United States T 800-628-7730 - F 314-991-1504 technical@GBiosciences.com - www.GBiosciences.com

1.4. Emergency telephone number

Emergency number : Chemtrec 1-800-424-9300 (USA/Canada), +1-703-527-3887 (Intl)

SECTION 2: Hazard(s) identification

2.1. Classification of the substance or mixture

Classification (GHS-US)

Skin Corr. 1B H314 - Causes severe skin burns and eye damage

Full text of H-phrases: see section 16

2.2. Label elements

GHS-US labeling

Hazard pictograms (GHS-US) :

GHS05

Signal word (GHS-US) : Danger

Hazard statements (GHS-US) : H314 - Causes severe skin burns and eye damage

Precautionary statements (GHS-US) : P260 - Do not breathe mist, spray P264 - Wash hands, forearms and face thoroughly after handling P280 - Wear eye protection, protective clothing, protective gloves P301 + P330 + P331 - If swallowed: rinse mouth. Do NOT induce vomiting P303 + P361 + P353 - If on skin (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower P304 + P340 - If inhaled: Remove person to fresh air and keep comfortable for breathing P305+P351+P338 - If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing P310 - Immediately call a a doctor, a POISON CENTER P321 - Specific treatment (see ... on this label) P363 - Wash contaminated clothing before reuse P405 - Store locked up P501 - Dispose of contents/container to an approved waste disposal plant

2.3. Other hazards

2.4. Unknown acute toxicity (GHS US)

Not applicable

SECTION 3: Composition/information on ingredients

3.1. Substances

3.2. Mixtures

08/06/2015 EN (English US) 2/7

Name Product identifier % Classification (GHS-US) sodium carbonate (CAS No) 497-19-8 2 - 5 Eye Irrit. 2A, H319

(2,2'-biquinoline)-4,4'-dicarboxylic acid, disodium salt (CAS No) 979-88-4 < 2 Eye Irrit. 2A, H319 sodium hydroxide (CAS No) 1310-73-2 < 2 Skin Corr. 1A, H314

SECTION 4: First aid measures

4.1. Description of first aid measures

First-aid measures general : Never give anything by mouth to an unconscious person. If you feel unwell, seek medical advice (show the label where possible). Dispose of rinse water as waste water. Call a physician immediately.

First-aid measures after inhalation : Remove person to fresh air and keep comfortable for breathing. Remove victim to fresh air and keep at rest in a position comfortable for breathing. Immediately call a poison center or doctor/physician.

First-aid measures after skin contact : Immediately call a poison center or doctor/physician. Rinse skin with water/shower.

Remove/Take off immediately all contaminated clothing. Call a physician immediately.

First-aid measures after eye contact : Immediately call a poison center or doctor/physician. Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a physician immediately.

First-aid measures after ingestion : Do NOT induce vomiting. Immediately call a poison center or doctor/physician. Rinse mouth.

Do NOT induce vomiting. Call a physician immediately.

4.2. Most important symptoms and effects, both acute and delayed

Symptoms/injuries : Causes severe skin burns and eye damage.

Symptoms/injuries after skin contact : Burns.

Symptoms/injuries after eye contact : Serious damage to eyes.

Symptoms/injuries after ingestion : Burns.

4.3. Indication of any immediate medical attention and special treatment needed

Treat symptomatically.

SECTION 5: Firefighting measures

5.1. Extinguishing media

suitable extinguishing media : Sand. Water spray. Dry powder. Foam. Carbon dioxide.

Unsuitable extinguishing media : Do not use a heavy water stream.

5.2. Special hazards arising from the substance or mixture

Reactivity : Thermal decomposition generates : Corrosive vapors.

5.3. Advice for firefighters

Firefighting instructions : Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire. Avoid (reject) fire-fighting water to enter environment.

Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory protection. Do not attempt to take action without suitable protective equipment. Self-contained breathing apparatus. Complete protective clothing.

SECTION 6: Accidental release measures

6.1. Personal precautions, protective equipment and emergency procedures

6.1.1. For non-emergency personnel

Emergency procedures : Ventilate spillage area. Evacuate unnecessary personnel. Avoid contact with skin and eyes. Do not breathe dust/fume/gas/mist/vapors/spray.

6.1.2. For emergency responders

Protective equipment : Do not attempt to take action without suitable protective equipment. Equip cleanup crew with proper protection. For further information refer to section 8 Exposure controls/personal protection" ".

Emergency procedures : Ventilate area.

6.2. Environmental precautions

Avoid release to the environment. Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters.

08/06/2015 EN (English US) 3/7

6.3. Methods and material for containment and cleaning up

Methods for cleaning up : Take up liquid spill into absorbent material. Soak up spills with inert solids, such as clay or diatomaceous earth as soon as possible. Collect spillage. Store away from other materials.

Other information : Dispose of materials or solid residues at an authorized site.

6.4. Reference to other sections

See Heading 8. Exposure controls and personal protection. For further information refer to section 8 : Exposure-controls/personal protection"".

SECTION 7: Handling and storage

7.1. Precautions for safe handling

Precautions for safe handling : Ensure good ventilation of the work station. Wash hands and other exposed areas with mild soap and water before eat, drink or smoke and when leaving work. Provide good ventilation in process area to prevent formation of vapor. Avoid contact with skin and eyes. Do not breathe dust/fume/gas/mist/vapors/spray. Wear personal protective equipment.

Hygiene measures : Wash ... thoroughly after handling. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Always wash hands after handling the product.

7.2. Conditions for safe storage, including any incompatibilities

Technical measures : Comply with applicable regulations.

Storage conditions : Keep only in the original container in a cool, well ventilated place away from : Keep container closed when not in use. Store locked up. Store in a well-ventilated place. Keep cool.

Incompatible products : Strong bases. strong acids.

Incompatible materials : Sources of ignition. Direct sunlight.

SECTION 8: Exposure controls/personal protection

8.1. Control parameters

sodium hydroxide (1310-73-2)

ACGIH ACGIH Ceiling (mg/m³) 2 mg/m³ (Sodium hydroxide; USA; Momentary value;

TLV - Adopted Value)

8.2. Exposure controls

Appropriate engineering controls : Ensure good ventilation of the work station.

Personal protective equipment : Avoid all unnecessary exposure.

Hand protection : Wear protective gloves.

Eye protection : Chemical goggles or face shield. Safety glasses.

Skin and body protection : Wear suitable protective clothing.

Respiratory protection : Wear approved mask.

Environmental exposure controls : Avoid release to the environment.

Other information : When using, do not eat, drink or smoke.

SECTION 9: Physical and chemical properties

9.1. Information on basic physical and chemical properties

Physical state : Liquid

Color : Mixture contains one or more component(s) which have the following colour(s):

Colourless to white White Colorless. White-yellow

Odor : There may be no odour warning properties, odour is subjective and inadequate to warn of overexposure.

Mixture contains one or more component(s) which have the following odour(s):

Odourless Almost odourless Mild odour

Odor threshold : No data available pH : No data available

Melting point : Not applicable

Freezing point : No data available

Boiling point : No data available

Flash point : No data available

Relative evaporation rate (butyl acetate=1) : No data available

08/06/2015 EN (English US) 4/7

Flammability (solid, gas) : No data available

Explosion limits : No data available

Explosive properties : No data available

Oxidizing properties : No data available

Vapor pressure : No data available

Relative density : No data available

Relative vapor density at 20 °C : No data available

Solubility : Water: Solubility in water of component(s) of the mixture :

• : 22 g/100ml • disodium tartrate,dihydrate: > 29 g/100ml • sodium hydroxide: 42 g/100ml

• (2,2'-biquinoline)-4,4'-dicarboxylic acid, disodium salt: 1.0 g/100ml

Log Pow : No data available

Self ignition temperature : No data available

Decomposition temperature : No data available

Viscosity : No data available

Viscosity, kinematic : No data available

Viscosity, dynamic : No data available

9.2. Other information

SECTION 10: Stability and reactivity

10.1. Reactivity

Thermal decomposition generates : Corrosive vapors.

10.2. Chemical stability

Not established.

10.3. Possibility of hazardous reactions

10.4. Conditions to avoid

Direct sunlight. Extremely high or low temperatures.

10.5. Incompatible materials

strong acids. Strong bases.

10.6. Hazardous decomposition products

fume. Carbon monoxide. Carbon dioxide. Thermal decomposition generates : Corrosive vapors.

SECTION 11: Toxicological information

11.1. Information on toxicological effects

Acute toxicity : Not classified sodium carbonate (497-19-8)

LD50 oral rat 2800 mg/kg (Rat; Experimental value)

LD50 dermal rabbit > 2000 mg/kg (Rabbit; Experimental value)

Skin corrosion/irritation : Causes severe skin burns and eye damage.

Serious eye damage/irritation : Not classified

Respiratory or skin sensitization : Not classified

Germ cell mutagenicity : Not classified

Based on available data, the classification criteria are not met

Carcinogenicity : Not classified

Reproductive toxicity : Not classified

Specific target organ toxicity (single exposure) : Not classified

Specific target organ toxicity (repeated exposure)

: Not classified

08/06/2015 EN (English US) 5/7

Aspiration hazard : Not classified

Potential Adverse human health effects and symptoms

: Based on available data, the classification criteria are not met.

Symptoms/injuries after skin contact : Burns.

Symptoms/injuries after eye contact : Serious damage to eyes.

Symptoms/injuries after ingestion : Burns.

SECTION 12: Ecological information

12.1. Toxicity

Ecology - general : Before neutralisation, the product may represent a danger to aquatic organisms.

LC50 fish 1 45.4 mg/l (LC50; Other; 96 h; Salmo gairdneri; Static system; Fresh water; Experimental value)

LC50 fish 1 300 mg/l (LC50; Other; 96 h; Lepomis macrochirus; Static system; Fresh water; Experimental value)

Threshold limit algae 1 242 mg/l (EC50; 5 days; Algae)

12.2. Persistence and degradability

Persistence and degradability Not established.

Persistence and degradability Biodegradability: not applicable. No (test)data on mobility of the substance available.

Biochemical oxygen demand (BOD) Not applicable

Chemical oxygen demand (COD) Not applicable

ThOD Not applicable

(2,2'-biquinoline)-4,4'-dicarboxylic acid, disodium salt (979-88-4)

Persistence and degradability Biodegradability in water: no data available.

Persistence and degradability Biodegradability: not applicable. Low potential for adsorption in soil.

ThOD Not applicable (inorganic)

12.3. Bioaccumulative potential

Bioaccumulative potential Not established.

Bioaccumulative potential No bioaccumulation data available.

(2,2'-biquinoline)-4,4'-dicarboxylic acid, disodium salt (979-88-4)

Bioaccumulative potential No bioaccumulation data available.

Log Pow -6.19 (Estimated value)

Bioaccumulative potential Low potential for bioaccumulation (Log Kow < 4).

12.4. Mobility in soil

12.5. Other adverse effects

Effect on the global warming : No known ecological damage caused by this product.

Other information : Avoid release to the environment.

08/06/2015 EN (English US) 6/7

SECTION 13: Disposal considerations

13.1. Waste treatment methods

Waste disposal recommendations : Dispose in a safe manner in accordance with local/national regulations. Dispose of contents/container to Dispose in a safe manner in accordance with local/national regulations.

Ecology - waste materials : Avoid release to the environment.

SECTION 14: Transport information

Department of Transportation (DOT)

In accordance with DOT

Transport document description : UN3082 Environmentally hazardous substances, liquid, n.o.s., 9, III

UN-No.(DOT) : UN3082

DOT Proper Shipping Name : Environmentally hazardous substances, liquid, n.o.s.

Department of Transportation (DOT) Hazard Classes

: 9 - Class 9 - Miscellaneous hazardous material 49 CFR 173.140

Hazard labels (DOT) : 9 - Class 9 (Miscellaneous dangerous materials)

Packing group (DOT) : III - Minor Danger

DOT Packaging Non Bulk (49 CFR 173.xxx) : 203

DOT Packaging Bulk (49 CFR 173.xxx) : 241

DOT Symbols : G - Identifies PSN requiring a technical name

DOT Special Provisions (49 CFR 172.102) : 8 - A hazardous substance that is not a hazardous waste may be shipped under the shipping description “Other regulated substances, liquid or solid, n.o.s.”, as appropriate. In addition, for solid materials, special provision B54 applies.

146 - This description may be used for a material that poses a hazard to the environment but does not meet the definition for a hazardous waste or a hazardous substance, as defined in

171.8 of this subchapter, or any hazard class as defined in Part 173 of this subchapter, if it is designated as environmentally hazardous by the Competent Authority of the country of origin, transit or destination.

173 - An appropriate generic entry may be used for this material.

335 - Mixtures of solids that are not subject to this subchapter and environmentally hazardous liquids or solids may be classified as “Environmentally hazardous substances, solid, n.o.s,” UN3077 and may be transported under this entry, provided there is no free liquid visible at the time the material is loaded or at the time the packaging or transport unit is closed. Each transport unit must be leak-proof when used as bulk packaging.

IB3 - Authorized IBCs: Metal (31A, 31B and 31N); Rigid plastics (31H1 and 31H2); Composite (31HZ1 and 31HA2, 31HB2, 31HN2, 31HD2 and 31HH2). Additional Requirement: Only liquids with a vapor pressure less than or equal to 110 kPa at 50 C (1.1 bar at 122 F), or 130 kPa at 55 C (1.3 bar at 131 F) are authorized, except for UN2672 (also see Special Provision IP8 in Table 2 for UN2672).

T4 - 2.65 178.274(d)(2) Normal............. 178.275(d)(3) TP1 - The maximum degree of filling must not exceed the degree of filling determined by the following: Degree of filling = 97 / 1 + a (tr - tf) Where: tr is the maximum mean bulk temperature during transport, and tf is the temperature in degrees celsius of the liquid during filling.

TP29 - A portable tank having a minimum test pressure of 1.5 bar (150.0 kPa) may be used provided the calculated test pressure is 1.5 bar or less based on the MAWP of the hazardous materials, as defined in 178.275 of this subchapter, where the test pressure is 1.5 times the

MAWP.

DOT Packaging Exceptions (49 CFR 173.xxx) : 155

DOT Quantity Limitations Passenger aircraft/rail

(49 CFR 173.27)

: No limit

DOT Quantity Limitations Cargo aircraft only (49

CFR 175.75)

: No limit

DOT Vessel Stowage Location : A - The material may be stowed ‘‘on deck’’ or ‘‘under deck’’ on a cargo vessel and on a passenger vessel.

Other information : No supplementary information available.

08/06/2015 EN (English US) 7/7

TDG

Transport by sea

Air transport

SECTION 15: Regulatory information

15.1. US Federal regulations

All components of this product are listed on the Toxic Substances Control Act (TSCA) inventory except for:

sodium hydroxide CAS No 1310-73-2 < 2%

(2,2'-biquinoline)-4,4'-dicarboxylic acid, disodium salt CAS No 979-88-4 < 2% sodium carbonate CAS No 497-19-8 2 - 5%

This product or mixture does not contain a toxic chemical or chemicals in excess of the applicable de minimis concentration as specified in 40 CFR §372.38(a) subject to the reporting requirements of section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 and 40 CFR Part 372.

Not listed on SARA Section 313 (Specific toxic chemical listings)

RQ (Reportable quantity, section 304 of EPA's List of Lists) :

1000 lb

15.2. International regulations

CANADA

EU-Regulations

National regulations

15.3. US State regulations

California Proposition 65 - This product does not contain any substances known to the state of California to cause cancer and/or reproductive harm

SECTION 16: Other information

Indication of changes : Revision - See : *.

Revision date : 08/06/2015

Other information : None.

Full text of H-phrases::

------ Eye Irrit. 2A Serious eye damage/eye irritation Category 2A

------ Skin Corr. 1A skin corrosion/irritation Category 1A

------ Skin Corr. 1B skin corrosion/irritation Category 1B

------ H314 Causes severe skin burns and eye damage

------ H319 Causes serious eye irritation

SDS US (GHS HazCom 2012)

This information is based on our current knowledge and is intended to describe the product for the purposes of health, safety and environmental requirements only. It should not therefore be construed as guaranteeing any specific property of the product

Copper Solution (BCA Reagent B)

Product name. : Copper Solution (BCA Reagent B)

Product code : 274C, 274C-B, 786-848

9800 Page Avenue Saint Louis, 63132-1429 - United States

Aquatic Chronic 2 H411 - Toxic to aquatic life with long lasting effects

Hazard pictograms (GHS-US) :

GHS09

Hazard statements (GHS-US) : H411 - Toxic to aquatic life with long lasting effects

Precautionary statements (GHS-US) : P273 - Avoid release to the environment P391 - Collect spillage P501 - Dispose of contents/container to ...

2.4. Unknown acute toxicity (GHS US)

copper(II) sulfate, pentahydrate (CAS No) 7758-99-8 2 - 5 Acute Tox. 3 (Oral), H301

First-aid measures general : Never give anything by mouth to an unconscious person. If you feel unwell, seek medical advice (show the label where possible).

First-aid measures after inhalation : Remove person to fresh air and keep comfortable for breathing. Assure fresh air breathing.

Allow the victim to rest.

08/06/2015 EN (English US) 2/7

First-aid measures after skin contact : Remove affected clothing and wash all exposed skin area with mild soap and water, followed by warm water rinse. Wash skin with plenty of water.

First-aid measures after eye contact : Rinse immediately with plenty of water. Obtain medical attention if pain, blinking or redness persist. Rinse eyes with water as a precaution.

First-aid measures after ingestion : Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention. Call a poison center or a doctor if you feel unwell.

Symptoms/injuries : Not expected to present a significant hazard under anticipated conditions of normal use.

suitable extinguishing media : Sand. Water spray. Dry powder. Foam. Carbon dioxide.

Reactivity : The product is non-reactive under normal conditions of use, storage and transport.

Firefighting instructions : Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire. Avoid (reject) fire-fighting water to enter environment.

Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory protection. Do not attempt to take action without suitable protective equipment. Self-contained breathing

Emergency procedures : Ventilate spillage area. Evacuate unnecessary personnel.

6.1.2. For emergency responders

Protective equipment : Do not attempt to take action without suitable protective equipment. Equip cleanup crew with proper protection. For further information refer to section 8 Exposure controls/personal

Avoid release to the environment. Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters.

For containment : Collect spillage.

Methods for cleaning up : Take up liquid spill into absorbent material. Soak up spills with inert solids, such as clay or

See Heading 8. Exposure controls and personal protection. For further information refer to section 8 : Exposure-controls/personal protection"".

Precautions for safe handling : Ensure good ventilation of the work station. Wear personal protective equipment. Wash hands and other exposed areas with mild soap and water before eat, drink or smoke and when leaving work. Provide good ventilation in process area to prevent formation of vapor.

Hygiene measures : Do not eat, drink or smoke when using this product. Always wash hands after handling the product.

Storage conditions : Keep only in the original container in a cool, well ventilated place away from : Keep container closed when not in use. Store in a well-ventilated place. Keep cool.

08/06/2015 EN (English US) 3/7

DNEL DNEL <=

Eye protection : Chemical goggles or safety glasses. Safety glasses.

Skin and body protection : Wear suitable protective clothing.

Respiratory protection : Wear approved mask.

Environmental exposure controls : Avoid release to the environment.

Color : Mixture contains one or more component(s) which have the following colour(s):

Blue

Odor : There may be no odour warning properties, odour is subjective and inadequate to warn of overexposure.

Mixture contains one or more component(s) which have the following odour(s):

Odourless

• copper(II) sulfate, pentahydrate: 23 g/100ml

The product is non-reactive under normal conditions of use, storage and transport.

08/06/2015 EN (English US) 4/7 fume. Carbon monoxide. Carbon dioxide.

copper(II) sulfate, pentahydrate (7758-99-8)

LD50 oral rat 300 mg/kg (Rat; OECD 401: Acute Oral Toxicity; Experimental value; 482 mg/kg bodyweight;

Rat)

LD50 dermal rabbit > 2000 mg/kg (Rabbit; Literature study; OECD 402: Acute Dermal Toxicity)

ATE US (oral) 300.000 mg/kg body weight

Skin corrosion/irritation : Not classified

Respiratory or skin sensitization : Not classified

Germ cell mutagenicity : Not classified

: Based on available data, the classification criteria are not met.

Ecology - general : Toxic to aquatic life with long lasting effects.

Ecology - water : Toxic to aquatic life with long lasting effects.

Threshold limit algae 2 0.368 mg/l (ErC50; OECD 201: Alga, Growth Inhibition Test; 72 h; Pseudokirchneriella subcapitata; Static system; Fresh water; Read-across)

Persistence and degradability May cause long-term adverse effects in the environment.

Persistence and degradability Biodegradability: not applicable. No (test)data on mobility of the substance available.

Biochemical oxygen demand (BOD) Not applicable

08/06/2015 EN (English US) 5/7

Bioaccumulative potential Bioaccumable.

Ecology - soil Toxic to flora.

Waste disposal recommendations : Dispose in a safe manner in accordance with local/national regulations. Dispose of contents/container to ...

Ecology - waste materials : Avoid release to the environment.

Transport document description : UN3082 Environmentally hazardous substances, liquid, n.o.s., 9, III

UN-No.(DOT) : UN3082

DOT Proper Shipping Name : Environmentally hazardous substances, liquid, n.o.s.

Department of Transportation (DOT) Hazard Classes

: 9 - Class 9 - Miscellaneous hazardous material 49 CFR 173.140

Hazard labels (DOT) : 9 - Class 9 (Miscellaneous dangerous materials)

Packing group (DOT) : III - Minor Danger

Dangerous for the environment : Yes

Marine pollutant : Yes

DOT Packaging Non Bulk (49 CFR 173.xxx) : 203

DOT Packaging Bulk (49 CFR 173.xxx) : 241

DOT Symbols : G - Identifies PSN requiring a technical name

08/06/2015 EN (English US) 6/7

DOT Special Provisions (49 CFR 172.102) : 8 - A hazardous substance that is not a hazardous waste may be shipped under the shipping description “Other regulated substances, liquid or solid, n.o.s.”, as appropriate. In addition, for solid materials, special provision B54 applies.

146 - This description may be used for a material that poses a hazard to the environment but does not meet the definition for a hazardous waste or a hazardous substance, as defined in

171.8 of this subchapter, or any hazard class as defined in Part 173 of this subchapter, if it is designated as environmentally hazardous by the Competent Authority of the country of origin, transit or destination.

173 - An appropriate generic entry may be used for this material.

335 - Mixtures of solids that are not subject to this subchapter and environmentally hazardous liquids or solids may be classified as “Environmentally hazardous substances, solid, n.o.s,” UN3077 and may be transported under this entry, provided there is no free liquid visible at the time the material is loaded or at the time the packaging or transport unit is closed. Each transport unit must be leak-proof when used as bulk packaging.

IB3 - Authorized IBCs: Metal (31A, 31B and 31N); Rigid plastics (31H1 and 31H2); Composite (31HZ1 and 31HA2, 31HB2, 31HN2, 31HD2 and 31HH2). Additional Requirement: Only liquids with a vapor pressure less than or equal to 110 kPa at 50 C (1.1 bar at 122 F), or 130 kPa at 55 C (1.3 bar at 131 F) are authorized, except for UN2672 (also see Special Provision IP8 in Table 2 for UN2672).

T4 - 2.65 178.274(d)(2) Normal............. 178.275(d)(3) TP1 - The maximum degree of filling must not exceed the degree of filling determined by the following: Degree of filling = 97 / 1 + a (tr - tf) Where: tr is the maximum mean bulk temperature during transport, and tf is the temperature in degrees celsius of the liquid during filling.

TP29 - A portable tank having a minimum test pressure of 1.5 bar (150.0 kPa) may be used provided the calculated test pressure is 1.5 bar or less based on the MAWP of the hazardous materials, as defined in 178.275 of this subchapter, where the test pressure is 1.5 times the

MAWP.

DOT Packaging Exceptions (49 CFR 173.xxx) : 155

DOT Quantity Limitations Passenger aircraft/rail

(49 CFR 173.27)

: No limit

DOT Quantity Limitations Cargo aircraft only (49

CFR 175.75)

: No limit

DOT Vessel Stowage Location : A - The material may be stowed ‘‘on deck’’ or ‘‘under deck’’ on a cargo vessel and on a passenger vessel.

Other information : No supplementary information available.

Not listed on the United States TSCA (Toxic Substances Control Act) inventory

08/06/2015 EN (English US) 7/7

Indication of changes : Revision - See : *.

------ Acute Tox. 3 (Oral) Acute toxicity (oral) Category 3

------ Aquatic Chronic 2 Hazardous to the aquatic environment - Chronic Hazard Category 2

------ H301 Toxic if swallowed

------ H411 Toxic to aquatic life with long lasting effects

This information is based on our current knowledge and is intended to describe the product for the purposes of health, safety and environmental requirements only. It should not therefore be construed as

BSA Solution

Product name. : BSA Solution

Product code : 786-006, 786-114, 786-920, 224B-B, 224B-C, 224B-O, 236B, 238B, 240B, 242B, 244B, 246B, 445A-A

9800 Page Avenue Saint Louis, 63132-1429 - United States

Not classified

No labeling applicable

2.4. Unknown acute toxicity (GHS US)

sodium azide (CAS No) 26628-22-8 < 2 Acute Tox. 2 (Oral), H300 Acute Tox. 1 (Dermal), H310 Aquatic Acute 1, H400 Aquatic Chronic 1, H410

First-aid measures general : Never give anything by mouth to an unconscious person. If you feel unwell, seek medical advice (show the label where possible).

First-aid measures after inhalation : Remove person to fresh air and keep comfortable for breathing. Assure fresh air breathing.

Allow the victim to rest.

First-aid measures after skin contact : Remove affected clothing and wash all exposed skin area with mild soap and water, followed by warm water rinse. Wash skin with plenty of water.

First-aid measures after eye contact : Rinse immediately with plenty of water. Obtain medical attention if pain, blinking or redness persist. Rinse eyes with water as a precaution.

First-aid measures after ingestion : Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention. Immediately call a poison center or doctor/physician. Specific treatment (see ... on this label). Call a poison center or a doctor if you feel unwell.

08/06/2015 EN (English US) 2/6

Symptoms/injuries after ingestion : Toxic if swallowed. Swallowing a small quantity of this material will result in serious health hazard.

suitable extinguishing media : Foam. Dry powder. Carbon dioxide. Water spray. Sand.

Reactivity : The product is non-reactive under normal conditions of use, storage and transport.

Firefighting instructions : Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire. Avoid (reject) fire-fighting water to enter environment.

Protection during firefighting : Do not enter fire area without proper protective equipment, including respiratory protection. Do not attempt to take action without suitable protective equipment. Self-contained breathing

Emergency procedures : Ventilate spillage area. Evacuate unnecessary personnel.

6.1.2. For emergency responders

Protective equipment : Do not attempt to take action without suitable protective equipment. Equip cleanup crew with proper protection. For further information refer to section 8 Exposure controls/personal

Prevent entry to sewers and public waters. Notify authorities if liquid enters sewers or public waters. Avoid release to the environment.

Methods for cleaning up : Take up liquid spill into absorbent material. Soak up spills with inert solids, such as clay or

See Heading 8. Exposure controls and personal protection. For further information refer to section 8 : Exposure-controls/personal protection"".

Precautions for safe handling : Ensure good ventilation of the work station. Wear personal protective equipment. Wash hands and other exposed areas with mild soap and water before eat, drink or smoke and when leaving work. Provide good ventilation in process area to prevent formation of vapor.

Hygiene measures : Do not eat, drink or smoke when using this product. Wash ... thoroughly after handling. Always wash hands after handling the product.

Storage conditions : Keep only in the original container in a cool, well ventilated place away from : Keep container closed when not in use. Store in a well-ventilated place. Keep cool.

sodium azide (26628-22-8)

ACGIH ACGIH Ceiling (mg/m³) 0.29 mg/m³ (Sodium azide, as sodium azide; USA;

Momentary value; TLV - Adopted Value)

08/06/2015 EN (English US) 3/6

ACGIH ACGIH Ceiling (ppm) 0.11 ppm (Sodium azide, as hydrazoic acid vapor;

USA; Momentary value; TLV - Adopted Value)

Eye protection : Chemical goggles or safety glasses. Safety glasses.

Skin and body protection : Wear suitable protective clothing.

Respiratory protection : Wear approved mask.

Environmental exposure controls : Avoid release to the environment.

Color : Colorless.

Odor : characteristic

• sodium azide: 42 g/100ml (17 °C) • : poorly soluble • sodium chloride: 31.7 g/100ml (20 °C)

The product is non-reactive under normal conditions of use, storage and transport.

08/06/2015 EN (English US) 4/6 fume. Carbon monoxide. Carbon dioxide.

ATE US (oral) 5.000 mg/kg body weight

ATE US (dermal) 5.000 mg/kg body weight

Skin corrosion/irritation : Not classified

Respiratory or skin sensitization : Not classified

Germ cell mutagenicity : Not classified

: Toxic if swallowed.

Symptoms/injuries after ingestion : Toxic if swallowed. Swallowing a small quantity of this material will result in serious health hazard.

Ecology - general : The product is not considered harmful to aquatic organisms nor to cause long-term adverse effects in the environment.

Ecology - water : Harmful to aquatic life with long lasting effects.

LC50 fish 1 0.8 mg/l (LC50; 96 h)

EC50 Daphnia 1 4.2 mg/l (EC50; 48 h)

Persistence and degradability May cause long-term adverse effects in the environment.

Persistence and degradability Biodegradability: not applicable.

Biochemical oxygen demand (BOD) Not applicable

08/06/2015 EN (English US) 5/6

Bioaccumulative potential Not bioaccumulative.

Waste disposal recommendations : Dispose in a safe manner in accordance with local/national regulations. Dispose of contents/container to ...

Ecology - waste materials : Avoid release to the environment. Hazardous waste due to toxicity.

No dangerous good in sense of transport regulations

Not listed on the United States TSCA (Toxic Substances Control Act) inventory Listed on SARA Section 313 (Specific toxic chemical listings)

RQ (Reportable quantity, section 304 of EPA's List of Lists) :

1000 lb

SARA Section 302 Threshold Planning Quantity (TPQ)

500 lb

Data sources : REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on classification, labeling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006.

08/06/2015 EN (English US) 6/6

------ Acute Tox. 1 (Dermal) Acute toxicity (dermal) Category 1

------ Acute Tox. 2 (Oral) Acute toxicity (oral) Category 2

------ Aquatic Acute 1 Hazardous to the aquatic environment - Acute Hazard Category 1

------ Aquatic Chronic 1 Hazardous to the aquatic environment - Chronic Hazard Category 1

------ H300 Fatal if swallowed

------ H310 Fatal in contact with skin

------ H400 Very toxic to aquatic life

------ H410 Very toxic to aquatic life with long lasting effects

This information is based on our current knowledge and is intended to describe the product for the purposes of health, safety and environmental requirements only. It should not therefore be construed as

Aldrich - 345245 Page 1 of 8

SIGMA-ALDRICH sigma-aldrich.com

SAFETY DATA SHEET

Version 3.11 Revision Date 05/23/2016

Print Date 03/27/2017

1. PRODUCT AND COMPANY IDENTIFICATION

1.1 Product identifiers

Product name : Phosphoric acid, 85 wt.%

Product Number : 345245 Brand : Aldrich

CAS-No. : 7664-38-2

1.2 Relevant identified uses of the substance or mixture and uses advised against

Identified uses : Laboratory chemicals, Synthesis of substances

1.3 Details of the supplier of the safety data sheet

Company : Sigma-Aldrich 3050 Spruce Street

SAINT LOUIS MO 63103

USA

Telephone : +1 800-325-5832 Fax : +1 800-325-5052

1.4 Emergency telephone number

Emergency Phone # : +1-703-527-3887 (CHEMTREC)

2. HAZARDS IDENTIFICATION

2.1 Classification of the substance or mixture

GHS Classification in accordance with 29 CFR 1910 (OSHA HCS) Corrosive to metals (Category 1), H290 Skin corrosion (Category 1B), H314 Serious eye damage (Category 1), H318

For the full text of the H-Statements mentioned in this Section, see Section 16.

2.2 GHS Label elements, including precautionary statements

Pictogram

Signal word Danger

Hazard statement(s) H290 May be corrosive to metals.

H314 Causes severe skin burns and eye damage.

H318 Causes serious eye damage.

Precautionary statement(s) P234 Keep only in original container.

P264 Wash skin thoroughly after handling.

P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.

P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303 + P361 + P353 IF ON SKIN (or hair): Remove/ Take off immediately all contaminated clothing. Rinse skin with water/ shower.

Aldrich - 345245 Page 2 of 8

P304 + P340 + P310 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/ physician.

P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor.

P363 Wash contaminated clothing before reuse.

P390 Absorb spillage to prevent material damage.

P405 Store locked up.

P406 Store in corrosive resistant stainless steel container with a resistant inner liner.

P501 Dispose of contents/ container to an approved waste disposal plant.

2.3 Hazards not otherwise classified (HNOC) or not covered by GHS - none

3. COMPOSITION/INFORMATION ON INGREDIENTS

3.2 Mixtures

Synonyms : Orthophosphoric acid

Formula : H3O4P

Molecular weight : 98.00 g/mol

Hazardous components

Component Classification Concentration

Phosphoric acid

CAS-No.

EC-No.

Index-No.

7664-38-2 231-633-2 015-011-00-6

Met. Corr. 1; Skin Corr. 1B;

Eye Dam. 1; H290, H314, H318

>= 70 - < 90 %

For the full text of the H-Statements mentioned in this Section, see Section 16.

4. FIRST AID MEASURES

4.1 Description of first aid measures

General advice Consult a physician. Show this safety data sheet to the doctor in attendance.Move out of dangerous area.

If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.

In case of skin contact Take off contaminated clothing and shoes immediately. Wash off with soap and plenty of water. Consult a physician.

In case of eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.Continue rinsing eyes during transport to hospital.

If swallowed Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.

4.2 Most important symptoms and effects, both acute and delayed The most important known symptoms and effects are described in the labelling (see section 2.2) and/or in section 11

4.3 Indication of any immediate medical attention and special treatment needed No data available

Aldrich - 345245 Page 3 of 8

5. FIREFIGHTING MEASURES

5.1 Extinguishing media

Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.

5.2 Special hazards arising from the substance or mixture

No data available

5.3 Advice for firefighters

Wear self-contained breathing apparatus for firefighting if necessary.

5.4 Further information

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions, protective equipment and emergency procedures Wear respiratory protection. Avoid breathing vapours, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.

For personal protection see section 8.

6.2 Environmental precautions

Do not let product enter drains.

6.3 Methods and materials for containment and cleaning up

Soak up with inert absorbent material and dispose of as hazardous waste. Keep in suitable, closed containers for disposal.

6.4 Reference to other sections

For disposal see section 13.

7. HANDLING AND STORAGE

7.1 Precautions for safe handling

Avoid inhalation of vapour or mist.

For precautions see section 2.2.

7.2 Conditions for safe storage, including any incompatibilities Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Storage class (TRGS 510): Non-combustible, corrosive hazardous materials

7.3 Specific end use(s)

Apart from the uses mentioned in section 1.2 no other specific uses are stipulated

8. EXPOSURE CONTROLS/PERSONAL PROTECTION

8.1 Control parameters

Components with workplace control parameters

Component CAS-No. Value Control parameters

Basis

Phosphoric acid 7664-38-2 TWA 1.000000 mg/m3

USA. ACGIH Threshold Limit Values

(TLV)

Remarks Upper Respiratory Tract irritation Eye irritation Skin irritation

TWA 1 mg/m3 USA. ACGIH Threshold Limit Values

(TLV)

Upper Respiratory Tract irritation Eye irritation Skin irritation

Aldrich - 345245 Page 4 of 8

STEL 3.000000

mg/m3

USA. ACGIH Threshold Limit Values

(TLV)

Upper Respiratory Tract irritation Eye irritation Skin irritation

STEL 3 mg/m3 USA. ACGIH Threshold Limit Values

(TLV)

Upper Respiratory Tract irritation Eye irritation Skin irritation

TWA 1.000000

mg/m3

USA. Occupational Exposure Limits (OSHA) - Table Z-1 Limits for Air Contaminants

TWA 1.000000

mg/m3

USA. NIOSH Recommended Exposure Limits

ST 3.000000

mg/m3

USA. NIOSH Recommended Exposure Limits

PEL 1 mg/m3 California permissible exposure limits for chemical contaminants (Title 8, Article 107)

STEL 3 mg/m3 California permissible exposure limits for chemical contaminants (Title 8, Article 107)

8.2 Exposure controls

Appropriate engineering controls Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday.

Personal protective equipment

Eye/face protection Tightly fitting safety goggles. Faceshield (8-inch minimum). Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).

Skin protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.

Full contact Material: Nitrile rubber Minimum layer thickness: 0.11 mm Break through time: 480 min Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)

Splash contact Material: Nitrile rubber Minimum layer thickness: 0.11 mm Break through time: 480 min Material tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M) data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 87300, e-mail sales@kcl.de, test method:

EN374

If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by an industrial hygienist and safety officer familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval for any specific use scenario.

Body Protection Complete suit protecting against chemicals, The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.

Aldrich - 345245 Page 5 of 8

Respiratory protection Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multi-purpose combination (US) or type ABEK (EN 14387) respirator cartridges as a backup to engineering controls.

If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).

Control of environmental exposure Do not let product enter drains.

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Information on basic physical and chemical properties

a) Appearance Form: liquid, clear

b) Odour No data available

c) Odour Threshold No data available

d) pH No data available

e) Melting point/freezing point

Melting point/range: 40 °C (104 °F) - lit.

f) Initial boiling point and boiling range

158 °C (316 °F) - lit.

g) Flash point No data available

h) Evaporation rate No data available

i) Flammability (solid, gas) No data available

j) Upper/lower flammability or explosive limits

No data available

k) Vapour pressure No data available

l) Vapour density No data available

m) Relative density 1.685 g/mL at 25 °C (77 °F)

n) Water solubility No data available

o) Partition coefficient: n-octanol/water

No data available

p) Auto-ignition temperature

No data available

q) Decomposition temperature

No data available

r) Viscosity No data available

s) Explosive properties No data available

t) Oxidizing properties No data available

9.2 Other safety information

10. STABILITY AND REACTIVITY

10.1 Reactivity

No data available

10.2 Chemical stability

Stable under recommended storage conditions.

10.3 Possibility of hazardous reactions

Aldrich - 345245 Page 6 of 8

10.4 Conditions to avoid

10.5 Incompatible materials

Strong bases, Powdered metals

10.6 Hazardous decomposition products

Hazardous decomposition products formed under fire conditions. - Thermal decomposition may produce toxic fumes of phosphorus oxides and/or phosphine Hazardous decomposition products formed under fire conditions. - Oxides of phosphorus Other decomposition products - No data available In the event of fire: see section 5

11. TOXICOLOGICAL INFORMATION

11.1 Information on toxicological effects

Acute toxicity No data available

Inhalation: No data available

Dermal: No data available

No data available

Skin corrosion/irritation No data available

Serious eye damage/eye irritation No data available

Respiratory or skin sensitisation No data available

Germ cell mutagenicity

Carcinogenicity

IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.

NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP.

OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA.

Reproductive toxicity No data available No data available

Specific target organ toxicity - single exposure No data available

Specific target organ toxicity - repeated exposure No data available

Aspiration hazard No data available

Additional Information RTECS: Not available burning sensation, Cough, wheezing, laryngitis, Shortness of breath, spasm, inflammation and edema of the larynx, spasm, inflammation and edema of the bronchi, pneumonitis, pulmonary edema, Material is extremely destructive to tissue of the mucous membranes and upper respiratory tract, eyes, and skin.

To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.

Aldrich - 345245 Page 7 of 8

Stomach - Irregularities - Based on Human Evidence Stomach - Irregularities - Based on Human Evidence (Phosphoric acid)

12. ECOLOGICAL INFORMATION

12.1 Toxicity

No data available

12.2 Persistence and degradability

No data available

12.3 Bioaccumulative potential

No data available

12.4 Mobility in soil

No data available

12.5 Results of PBT and vPvB assessment

PBT/vPvB assessment not available as chemical safety assessment not required/not conducted

12.6 Other adverse effects

13. DISPOSAL CONSIDERATIONS

13.1 Waste treatment methods

Product Offer surplus and non-recyclable solutions to a licensed disposal company. Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.

Contaminated packaging Dispose of as unused product.

14. TRANSPORT INFORMATION

DOT (US)

UN number: 1805 Class: 8 Packing group: III Proper shipping name: Phosphoric acid solution Reportable Quantity (RQ):

Poison Inhalation Hazard: No

IMDG

UN number: 1805 Class: 8 Packing group: III EMS-No: F-A, S-B Proper shipping name: PHOSPHORIC ACID SOLUTION

IATA

UN number: 1805 Class: 8 Packing group: III Proper shipping name: Phosphoric acid, solution

15. REGULATORY INFORMATION

SARA 302 Components No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.

SARA 313 Components This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.

SARA 311/312 Hazards Acute Health Hazard, Chronic Health Hazard

Massachusetts Right To Know Components

Phosphoric acid

CAS-No.

7664-38-2

Revision Date 1993-04-24

Aldrich - 345245 Page 8 of 8

Pennsylvania Right To Know Components

Phosphoric acid

CAS-No.

7664-38-2

Revision Date 1993-04-24

Water 7732-18-5

New Jersey Right To Know Components

Phosphoric acid

CAS-No.

7664-38-2

Revision Date 1993-04-24

Water 7732-18-5

California Prop. 65 Components This product does not contain any chemicals known to State of California to cause cancer, birth defects, or any other reproductive harm.

16. OTHER INFORMATION

Full text of H-Statements referred to under sections 2 and 3.

Eye Dam. Serious eye damage H290 May be corrosive to metals.

H314 Causes severe skin burns and eye damage.

H318 Causes serious eye damage.

Met. Corr. Corrosive to metals Skin Corr. Skin corrosion

HMIS Rating Health hazard: 3 Chronic Health Hazard: * Flammability: 0 Physical Hazard 0

NFPA Rating Health hazard: 3 Fire Hazard: 0 Reactivity Hazard: 0

Further information Copyright 2016 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies for internal use only.

The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any damage resulting from handling or from contact with the above product. See www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional terms and conditions of sale.

Preparation Information Sigma-Aldrich Corporation Product Safety – Americas Region 1-800-521-8956

Version: 3.11 Revision Date: 05/23/2016 Print Date: 03/27/2017

Sigma-Aldrich - 322415 Page 1 of 10

SIGMA-ALDRICH sigma-aldrich.com

Version 6.1 Revision Date 03/13/2015

Print Date 04/30/2016

1. PRODUCT AND COMPANY IDENTIFICATION

1.1 Product identifiers

Product name :…