Attachment 3 Hazardous Waste Inventory List.pdf

Hazardous Waste Disposal United States Department of Agriculture Agricultural Research Service Carl Hayden Bee Research Center 2000 E. Allen Rd. Tucson, AZ 85719

A quote is requested to remove the following containers from our Location for hazardous waste disposal. The contents of the following containers is predominately bulk-waste (i.e., pipette tips, Eppendorf tubes, Kim wipes, etc.) that was used in specific lab processes and has therefore been in contact with the substances listed for each corresponding container. There is very little, if any, liquid substance remaining in each container, unless otherwise noted.

Each container is either a 5- or 6.5-gallon plastic bucket. In some cases, the bulk-waste is contained in plastic bags, which were then placed in a plastic bucket. In other cases, the bulk-waste was deposited directly into a plastic bucket. Additional containers are not needed as part of this quote.

Location Disposal Identification

Lab Process / Assay Substances Used

D-011 Bicinchoninic Acid (BCA) Assay -Reagent B (sulfuric acid copper salt hydrate) -HALT Protease Inhibitor

D-016 BCA Assay Same as previous G-034 BCA Assay Same as previous G-035 BCA Assay Same as previous G-036 BCA Assay Same as previous G-037 BCA Assay Same as previous G-038 BCA Assay Same as previous G-039 BCA Assay Same as previous G-040 BCA Assay Same as previous G-041 BCA Assay Same as previous G-005 Bradford Assay -Methyl alcohol

-Phosphoric acid D-003 Chromic Acid Assay -Chromic acid

-Chloroform -Methanol

D-004 Chromic Acid Assay Same as previous G-064 Colorimetric Plate Bulk Waste -Bicinchoninic acid

-4-Nitrophenol -Amplex Red

G-061 Cyclohexane and DNA Extraction Waste -Cyclohexane -Guanidine hydrochloride -Acetic acid -Ribonuclease

Lab Process / Assay Substances Used

G-062 Cyclohexane and DNA Extraction Waste Same as previous G-056 Enzyme Immuno Assay (EIA) + Enzyme-

Linked Immunosorbent Assay (ELISA) + Bicinchoninic Acid Assay

-EIA Wash Buffer (contained liquid) -EIA Tracer Dye -EIA Antiserum Dye -Ellman’s Reagent -Practice EIA Standard -BCA Protein Assay Reagent B

G-057 EIA + ELISA + BCA Assay Same as previous G-058 EIA + ELISA + BCA Assay Same as previous G-049 Ethidium Bromide Agarose Gel Waste -Ethidium bromide G-050 Ethidium Bromide Bulk Waste -Ethidium bromide D-009 EZ Faast Assay -Chloroform

-Isooctane -N-Propanol -Sodium hydroxide

D-001 Fatty Acid Methyl Ester (FAME) Assay -Hexane -Chloroform -Methanol -Hydrochloric acid -Toluene

D-007 FAME Assay Same as previous D-008 FAME Assay Same as previous D-013 FAME Assay Same as previous D-015 FAME Assay Same as previous G-003 FAME Assay Same as previous G-051 FAME Assay Same as previous G-052 FAME Assay Same as previous G-065 Giemsa Stain Bulk Waste -Methanol G-047 Lysis Buffer Bulk Waste -Guanidine thiocyanate G-045 Nucleic Acid/Tri-Reagent + RNEasy RNA

Extraction Assay -Guanidine thiocyanate -Beta-Mercaptoethanol -Phenol -Chloroform

G-046 Nucleic Acid/Tri-Reagent + RNEasy RNA Extraction Assay

Same as previous

G-053

Nucleic Acid/Tri-Reagent + RNEasy RNA Extraction Assay

Same as previous

G-054 Nucleic Acid/Tri-Reagent + RNEasy RNA Extraction Assay

Same as previous

G-066 Pesticide (Insect growth regulators) Waste -Methoxyfenozide -Methoprene -Pyriproxyfen -Spirodiclofen -Makisterone -Octopamine

Lab Process / Assay Substances Used

G-008 Protein Carbonyl Assay -DNPH Solution -TCA Solution -Streptozocin Solution -6M Guanidine Solution

G-059 Protein Extraction Assay -Thiourea -Protease Inhibitor cocktail -NP-40 alternative -Sodium hydroxide

G-060 Protein Extraction Assay Same as previous G-001 RNA/DNA Extraction/Lysis Buffer Assay -Phenol

-Chloroform -Guanidine thiocyanate -Beta-Mercaptoethanol

G-002 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-004 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-007 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-042 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-043 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-044 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-048 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-055 RNA/DNA Extraction/Lysis Buffer Assay Same as previous G-006 Thibarbituric Acid Reactive Substances

(TBARS) Assay -Acetic acid -Sodium hydroxide solution -Thiobarbituric acid -Sodium dodecyl sulfate

G-063 TBARS Assay Same as previous D-010 Trimethylsilyl Iodide (TMSI) Derivitization -Trimethylsilyl iodide

-Chloroform -Methanol -Pyridine -Potassium hydroxide -Hexane -Sodium sulfate

D-319 Vanillin Assay -Chloroform -Methanol -Sulfuric acid -Vanillin -Phosphoric acid

G-009 Vanillin Assay Same as previous G-010 Vanillin Assay Same as previous G-011 Vanillin Assay Same as previous G-012 Vanillin Assay Same as previous G-013 Vanillin Assay Same as previous G-014 Vanillin Assay Same as previous G-015 Vanillin Assay Same as previous

Lab Process / Assay Substances Used

G-016 Vanillin Assay Same as previous G-017 Vanillin Assay Same as previous G-018 Vanillin Assay Same as previous G-019 Vanillin Assay Same as previous G-020 Vanillin Assay Same as previous G-021 Vanillin Assay Same as previous G-022 Vanillin Assay Same as previous G-023 Vanillin Assay Same as previous G-024 Vanillin Assay Same as previous G-025 Vanillin Assay Same as previous G-026 Vanillin Assay Same as previous G-027 Vanillin Assay Same as previous G-028 Vanillin Assay Same as previous G-029 Vanillin Assay Same as previous G-030 Vanillin Assay Same as previous G-031 Vanillin Assay Same as previous G-032 Vanillin Assay Same as previous G-033 Vanillin Assay Same as previous