Attachment 1 Statemetn of Work SOW 1232SA25Q0591.pdf
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- Solicitation number
- 1232SA25Q0591
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This Statement of Work (SOW) details a genotyping project for the USDA Germplasm Enhancement of Maize (GEM) Project located in Ames, IA. The project aims to determine genomic contributions of exotic maize and assess how genomic selection impacts genetic diversity. The contractor is required to perform kernel chipping of corn seeds, extract and quality control DNA, construct a DNA library, develop a marker panel targeting approximately 45,000 SNPs across 10 corn chromosomes, conduct sequencing with quality control, and provide bioinformatics analysis with a user-friendly interface for comparative genetic analysis.
Key technical requirements include creating a marker panel evenly distributed across corn chromosomes with a ~60Kb step size, developed from diverse corn germplasm, and capable of capturing over 250,000 SNPs. The project has a 180-day completion timeline from seed receipt, with generated data to be owned by the USDA and made publicly available. Bid evaluation will focus on technical capabilities, price, and delivery availability. The technical point of contact is Adam Vanous (515-231-3517, adam.vanous@usda.gov), and the project seeks to enhance temperate corn's genetic diversity for traits like seed composition, disease resistance, drought tolerance, and pest resistance.
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Statement of Work Project: Genotyping Corn Doubled Haploid Lines
GENERAL INFORMATION
1.0 Scope of Work: The USDA Germplasm Enhancement of Maize (GEM) Project, located at the North Central Regional Plant Introduction Station in Ames, IA, is conducting a study with the objective of determining genomic contributions of exotic maize and how this is affected when using genomic selection.
2.0 Background: The Germplasm Enhancement of Maize Project adapts exotic maize germplasm from around the world to the U.S. Corn-Belt. Temperate corn lacks diversity for many key traits: seed composition, disease resistance, drought tolerance, and pest resistance. The GEM corn lines, which incorporate exotic tropical diversity, have the potential to increase the selectable variation for these key traits.
Genotyping GEM material would allow for the quantification of exotic maize contributions and allow for a more focused approach to breeding. This genetic data would also aid in the development of a scheme that would allow for the more efficient use of genomic selection to speed up the breeding cycles, all while increasing useful genetic diversity.
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:
• Plant material: Capable of kernel chipping corn seeds.
• DNA: DNA extraction from kernel chipping. DNA quality control measures must be practiced, ensuring genotyping quality.
• Library Construction: Must be able to provide DNA library construction.
• Marker Panel: Marker panel capable of targeting ~45,000 SNPs and capturing over
250,000 SNPs. Marker panel must be evenly distributed across the 10 corn chromosomes. The step size should be ~60Kb. Marker panel should be developed from diverse corn germplasm.
• Sequencing: Quality control for sequence reads must be practiced. Sequence trimming to ensure quality is a must.
• Bioinformatics: Bioinformatics analysis must be included. Interface should include a user-friendly interface for reference sequence comparative analysis, sequencing depth and coverage statistics, detection and annotation of variation loci, including SNPs, indels, STRs/SSRs.
4.0 Government Furnished: Corn seeds for genotyping
5.0 Deliverable/Schedule: Work should be completed within 180 days of receiving corn seeds.
6.0 Evaluate Factors: Bids will be evaluated on technical capability outlined in Section
3.0, price, and delivery availability.
7.0 Data Rights: Generated data will be owned by the USDA and will be made publicly available.
8.0 Technical Point of Contact: Adam Vanous, 515-231-3517, adam.vanous@usda.gov
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