Attachment 1 - Statement of Work.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project: High-throughput CRISPR Interference (CRISPRi) screening to understand the pathobiology of Acral lentiginous melanoma (ALM) and to determine genes required for ALM survival.

Statement of Need and Purpose: The purpose of this procurement is the acquisition of six dCas9-KRAB cell lines.

Background Information and Objective: The National Institutes of Health has established an RNAi/CRIPSR Cas9 screening facility to provide intramural researchers with access to the equipment and expertise necessary to conduct genome-wide RNAi/ CRIPSR Cas9 screens in mammalian cells. This facility works as a collaborative resource for doing RNAi/ CRIPSR Cas9 assay development, screening, and initial follow-up. The facility works with investigators to develop and optimize their assays for screening, conducts the screens and related informatics analyses, and then performs follow-up screens on promising leads. Further work is done with the PI to do the downstream biology required to get the quality publications and the kind of groundbreaking results needed. This request is required for this facility to develop a flexible functional genomics platform that offers complementary and orthogonal RNAi and CRISPR Cas9 screens to NIH intramural investigators.

The required dCas9 cell lines will be used to repress the target gene expression when applied in a CRISPRi screen. This item is needed to facilitate the RNAi/ CRIPSR Cas9 screening program.

Period of Performance: 4/3/2024- 6/26/2024 or 12 weeks after receipt of the order

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:

Specific Requirements:

The Contractor, with the cell lines provided by the customer, will provide 6 dCas9 cell lines and will use their cell line engineering services to make cell lines with inducible targeted gene inhibitions.

dCas9-KRAB lines for CRISPi/ 6 dCas9 cell lines for CRISPR Interference (CRISPRi) screening:

• MB4667 dCas9-KRAB-Blast

• MB2204 dCas9-KRAB-Blast

• MB2724 dCas9-KRAB-Blast

• MB3997 dCas9-KRAB-Blast

• YUHIMO 09-1365C dCas9-KRAB-Blast

• YUSEEP-M 16-3517C dCas9-KRAB-Blast

GOVERNMENT RESPONSIBILITIES

The NCATS Functional Genomics Lab will provide Cellecta with the parental cell lines that they will work with.

DELIVERY OR DELIVERABLES

12 weeks days from the date of receipt of the order

Delivery Address:

National Institutes of Health (NIH), National Center for Advanced Translational Sciences (NCATS) 9800 Medical Center Drive Bldg C, Rm 346E Rockville, MD 20850

REPORTING REQUIREMENTS

N/A

OTHER CONSIDERATIONS

Travel:

N/A

Key Personnel:

N/A

Information System Security Plan:

N/A

Data Rights:

N/A

Section 508—Electronic and Information Technology Standards:

Publications and Publicity: