Attachment 1 - Statement of Work.pdf
PDF 155 KB Posted
- Attached to
- Long Read Sequencing of 24 Daucus carota samples
- Solicitation number
- 12805B23Q0932
About this file
This document outlines a Statement of Work for a federal contract solicitation. The USDA Agricultural Research Service is seeking long read DNA sequencing services for 24 Daucus carota genomic DNA samples to better understand the genetics and genomics of the Daucus taxon. The contractor will be required to evaluate DNA quality, construct libraries from the 24 samples with separate barcoding for identification, size select libraries to ensure minimum 15,000-20,000 base read lengths, sequence each sample to provide a minimum of 61010 bases of sequence data for each sample with an average read length of 15,000 bases and 90% of bases at Q30 accuracy and median read accuracy of Q30. Deliverables are to include the sequence data and quality reports electronically delivered to the Vegetable Crops Research Unit contact by October 30, 2023 either via ftp, rsync, globus or other standard protocols. The related federal contract opportunity is solicitation number 12805B23Q0932 for long read sequencing of the same 24 Daucus carota samples through the USDA Agricultural Research Service Plains Area.
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| File | Type | Posted |
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| Attachement 2 - Wage Rates.pdf | ||
| CLAUSES AND PROVISIONS - 12805B23Q0932.pdf |
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Statement of Work (SOW)
Project: Long Read DNA Sequencing of 24 Daucus carota samples for Vegetable Crops Research Unit
GENERAL INFORMATION
1.0 Scope of Work:
Provide long read DNA sequencing service for 24 Daucus carota genomic DNA samples.
2.0 Background:
The USDA-ARS will use this data to better understand the genetics and genomics of the Daucus taxon.
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:
Evaluate DNA for quality. Samples will be provided to the vendor by overnight shipment.
Construct libraries from 24 Daucus carota genomic DNA samples. Each sample will be separately barcoded for identification after sequencing.
Libraries will be size selected to insure minimum read length of 15,000- 20,000 bases.
Sequence each sample.
Results must include:
Quality Control analysis of each sample.
Minimum of 6×1010 bases (60 Gbases) of sequence data for each of the 24 samples.
Average read length should be a minimum of 15,000 bases.
Accuracy should provide a mean 90% of bases ≥Q30 and median read accuracy ≥Q30.
4.0 Deliverables / Schedule:
The product to be delivered is the sequence data and quality reports in electronic format. Delivery by October 30, 2023. Data will be delivered to the Vegetable Crops Research Unit in care of Douglas Senalik douglas.senalik@usda.gov by electronic file transfer, either by ftp, rsync, globus, or other standard protocols.
Vendor also has the option to provide data on a hard drive shipped to Vegetable Crops Research Unit, in care of Douglas Senalik, 1575 Linden Dr., Madison WI 53706.
5.0 Security Requirements:
Vendor will coordinate with on-site IT for data delivery.
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