Attachment 1 - Salient Characteristics.pdf
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- Attached to
- Capillary Electrophoresis (CE) Instrument
- Solicitation number
- 20341426Q00033
About this file
This document is a Salient Characteristics attachment for a federal procurement of a Capillary Electrophoresis (CE) instrument by the Beverage Alcohol Laboratory (BAL) of the Alcohol and Tobacco Tax and Trade Bureau (TTB), located in Beltsville, Maryland.
The BAL seeks a CE instrument capable of analyzing, separating, and quantitating eight organic acids in alcoholic beverages: tartaric acid, acetic acid, malic acid, lactic acid, citric acid, gluconic acid, succinic acid, and fumaric acid. The instrument will support determination of standard of identity for formula submissions, product integrity investigations, and the Alcohol Beverage Sampling Program. Future expansion capabilities should include analysis of sugars (erythritol, fructose, glucose, sucrose, maltose, and lactose) and cyanide detection. The instrument must operate under specified conditions: 100 mbar injection pressure, 20°C capillary temperature, -25 kV voltage, and 350/20 nm detector signal with 200/10 nm reference. Required specifications include a programmable pressure system (-100 to +100 mbar), safety features (current leak detection, liquid leak sensor, high voltage safety sensors), self-correcting injection system with programmable range up to 10,000 seconds, 50-position autosampler with temperature control (10-40°C), satellite station for buffer replenishment, and 100 μL, 1 mL, and 2 mL vial compatibility. The capillary cassette must include a high-speed forced-air cooler with Peltier element, temperature range 10°C below ambient to 60°C, and support 365 μm outer diameter capillaries with minimum 33 cm length. The detector must be a real-time UV-Visible diode-array detector (190-600 nm) with 1 nm wavelength accuracy, 0.025 to 10 second response time, 1 μM sensitivity, and baseline noise less than 50 μAU at 2 second response time. The system must operate under Windows XP (SP3) with time-programmable parameters, constant voltage/current/power modes, voltage range 0 to ±30 kV, current 0-300 μA, and power 0-6 W. Power requirements are 100-240 V line voltage, maximum 300W, 50-60 Hz frequency. The winning proposal must include five line items: CPU with monitor and printer, familiarization training at installation, on-site method consulting, an Organic Acids Kit, and the capability to add multiple detectors in the future.
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Capillary Electrophoresis (CE) Instrument AƩachment 1: Salient CharacterisƟcs
The Beverage Alcohol Laboratory (BAL) located at 6000 Ammendale Road, Beltsville, Maryland, is part of the ScienƟfic Services Division of TTB. The laboratory provides technical experƟse and assistance to other divisions within TTB by analyzing approximately 1,200 alcoholic beverage samples (wine, malt beverages, and disƟlled spirits) in a year across a variety of programs.
TTB’s Beverage Alcohol Laboratory (BAL) is interested in purchasing a capillary electrophoresis
(CE) instrument that would be capable of performing BAL’s previously developed test method for determining organic acids in alcoholic beverages. This would be used for determinaƟon of standard of idenƟty for Formula submissions with lab analysis, Product Integrity invesƟgaƟons, and the Alcohol Beverage Sampling Program (ABSP). The instrument also allows BAL to possibly expand their capabiliƟes to determine sugars (erythritol, fructose, glucose, sucrose, maltose, and lactose) used in nutriƟonal labeling assessment, and cyanide in alcoholic beverages which is a limited ingredient.
Method Parameters
The instrument should be able to analyze, separate, and quanƟtate the following organic acids in alcoholic beverages such as wines, malt beverages, and disƟlled spirits:
Tartaric Acid AceƟc Acid
Malic Acid LacƟc Acid
Citric Acid Gluconic Acid
Succinic Acid Fumaric Acid
Capillary Electrophoresis System AƩachment 1: Salient CharacterisƟcs
Instrument CondiƟons include:
Parameter Value
Injection Pressure 100 mbar
Capillary Temperature 20°C
Voltage -25 kV
Detector Signal 350/20 nm, Reference 200/10nm
Instrument Specifications
Pressure system
Programmable with -100 to +100 mbar on inlet
Flushing with 1 bar or with high pressure 2–12 bar
Vial pressurization with high pressure 2–12 bar on inlet and/or outlet
Safety features
Current leak detection low current limit
Liquid leak sensor
Safety sensors at door and cover disabling high voltage
Vial sensor
Injection modes
Self correcting injection system with injection from inlet or outlet
Programmable range up to 10,000 seconds
Pressure –100 to +100 mbar
Electrokinetic –30 to +30 kV
Autosampler/ fraction collector
50-position carousel
All vials are randomly accessible from inlet or outlet end of capillary
Temperature control with external waterbath with vial temperature from 10–40 °C (non-condensing conditions, minimum waterbath temperature +1 °C)
Replenishment
Satellite station for refilling of inlet or outlet buffer vials with fresh buffer for automatic, continuous operation.
Selectable buffer levelling.
Vials
100 μL (polypropylene or glass) with resealing snap caps
1 mL (polypropylene) with resealing snap caps
2 mL (glass) with resealing snap caps
Capillary cassette
High-speed forced-air cooler with Peltier element
Temperature range 10 °C below ambient, up to 60 °C (min. 10 °C cassette temperature)
Minimum total capillary length 33 cm
Capillary compatibility 365 µm o.d.
Detector
Real time UV-Visible diode-array detector (190–600 nm)
Temperature controlled
Wavelength accuracy
1 nm
Response time 0.025 to 10 s
Light source prealigned deuterium or high brightness lamp
Signals up to eight signals simultaneously, full spectral acquisition with Agilent ChemStation
Sensitivity 1 μM 4-hydroxy-aceto-phenon injected at 50 mbar*5 sec, 3 x 50 µm bubble cell capillary, signal/noise >6* (50 mM borate buffer pH 9.3, 25 kV)
Baseline noise <50 μAU (at 2 sec response time)*
Linear dynamic range
1x104 (3x50 μm bubble cell capillary)*
Raw data channels
Detector signals and spectrum, voltage, current, leak current, power, cassette temperature, pressure, lamp voltage and detector temperature
Diagnostic functions
RFID tag for lamp, early maintenance feedback, supported by Agilent Lab Advisor software with integrated diagnosis test suite
System control
Operating with graphical user interface under WindowsXP (SP3)
Time programmable parameters
Time programmable parameters: voltage, current, power, polarity, pressure, inlet and outlet vial, capillary temperature, pre and post-run conditioning with pressure and/or voltage, Replenishment, fraction collection**
Electrophoresis power
Voltage range setable 0 to ± 30 kV supply
Current setable 0–300 μA
Power setable 0–6 W
Operation under constant voltage, current or power Programmable polarity switch
CE specific software
Mobility report, time corrected areas, pI calibration and bio polymer size calibration
Power requirements
Line voltage 100-240 V, max. 300W
Line frequency 50-60 Hz
Acquisition Requirements
The winning proposal will meet the conditions specified above in the Method Parameters and
Instrument Specifications sections. It will also include the following items in line item format:
1. CPU including monitor and printer
2. Familiarization at installation
3. Method consulting on site
4. Organic Acids Kit
5. The ability to add multiple detectors if needed in the future.
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