Amnd 0002 Salient Characteristics.pdf

Request for Brand Name or Equal Capillary Electrophoresis (CE) Instrument: Agilent G7100A, See below specifications.

The Beverage Alcohol Laboratory (BAL) located at 6000 Ammendale Road, Beltsville, Maryland, is part of the Scientific Services Division of TTB. The laboratory provides technical expertise and assistance to other divisions within TTB by analyzing approximately 1,200 alcoholic beverage samples (wine, malt beverages, and distilled spirits) in a year across a variety of programs.

TTB’s Beverage Alcohol Laboratory (BAL) is interested in purchasing a capillary electrophoresis (CE) instrument that would be capable of performing BAL’s previously developed test method for determining organic acids in alcoholic beverages. This would be used for determination of standard of identity for Formula submissions with lab analysis, Product Integrity investigations, and the Alcohol Beverage Sampling Program (ABSP). The instrument also allows BAL to possibly expand their capabilities to determine sugars (erythritol, fructose, glucose, sucrose, maltose, and lactose) used in nutritional labeling assessment, and cyanide in alcoholic beverages which is a limited ingredient.

Method Parameters

The instrument should be able to analyze, separate, and quantitate the following organic acids in alcoholic beverages such as wines, malt beverages, and distilled spirits:

Tartaric Acid Malic Acid Citric Acid Succinic Acid Acetic Acid Lactic Acid Gluconic Acid Fumaric Acid

Capillary Electrophoresis System Attachment 1: Salient Characteristics

Instrument Conditions include:

Parameter Value

Injection Pressure 100 mbar

Capillary Temperature 20°C

Voltage -25 kV

Detector Signal 350/20 nm, Reference 200/10nm

Instrument Specifications

Pressure system Programmable with -100 to +100 mbar on inlet Flushing with 1 bar or with high pressure 2–12 bar Vial pressurization with high pressure 2–12 bar on inlet and/or outlet

Safety features Current leak detection low current limit Liquid leak sensor Safety sensors at door and cover disabling high voltage Vial sensor

Injection modes Self-correcting injection system with injection from inlet or outlet Programmable range up to 10,000 seconds Pressure –100 to +100 mbar Electrokinetic –30 to +30 kV

Autosampler/ fraction collector 50-position carousel All vials are randomly accessible from inlet or outlet end of capillary Temperature control with external waterbath with vial temperature from 10–40 °C (non-condensing conditions, minimum waterbath temperature +1 °C)

Replenishment Satellite station for refilling of inlet or outlet buffer vials with fresh buffer for automatic, continuous operation.

Selectable buffer levelling.

Vials 100 μL (polypropylene or glass) with resealing snap caps 1 mL (polypropylene) with resealing snap caps 2 mL (glass) with resealing snap caps

Capillary cassette High-speed forced-air cooler with Peltier element Temperature range 10 °C below ambient, up to 60 °C (min. 10 °C cassette temperature) Minimum total capillary length 33 cm Capillary compatibility 365 µm o.d.

Detector Real time UV-Visible diode-array detector (190–600 nm) Temperature controlled Wavelength accuracy

1 nm

Response time 0.025 to 10 s Light source prealigned deuterium or high brightness lamp Signals up to eight signals simultaneously, with full spectral acquisition

Sensitivity 1 μM 4-hydroxy-aceto-phenon injected at 50 mbar*5 sec, 3 x 50 µm bubble cell capillary, signal/noise >6* (50 mM borate buffer pH 9.3, 25 kV)

Baseline noise <50 μAU (at 2 sec response time)* Linear dynamic range

1x104 (3x50 μm bubble cell capillary)*

Raw data channels Detector signals and spectrum, voltage, current, leak current, power, cassette temperature, pressure, lamp voltage and detector temperature Diagnostic functions RFID tag for lamp, early maintenance feedback, supported by integrated diagnosis test suite System control Operating with graphical user interface under Windows 11

Time programmable parameters

Time programmable parameters: voltage, current, power, polarity, pressure, inlet and outlet vial, capillary temperature, pre- and post-run conditioning with pressure and/or voltage, Replenishment, fraction collection**

Electrophoresis power Voltage range setable 0 to ± 30 kV supply Current setable 0–300 μA Power setable 0–6 W Operation under constant voltage, current or power Programmable polarity switch

CE specific software

Mobility report, time corrected areas, pI calibration and bio polymer size calibration Power requirements Line voltage 100-240 V, max. 300W Line frequency 50-60 Hz

Acquisition Requirements

The winning proposal will meet the conditions specified above in the Method Parameters and Instrument Specifications sections. It will also include the following items in line-item format:

1. CPU including monitor and printer

2. Familiarization at installation

3. Method consulting on site

4. Organic Acids Kit

5. The ability to add multiple detectors if needed in the future.