Attachment 1 Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Brand Name or Equal: Neurostar Drill and Injection Micro-robot for Performing Intracranial Injections in Mice

National Institute for Neurological Disorders and Stroke, Cellular Neurophysiology Section

1. Statement of Need and Purpose: The National Institutes of Health (NIH) requires a brand name or equal Neurostar Drill and Injection Micro-robot automated stereotaxic injection system. This equipment will be used to perform intracranial injections and to make cranial windows into mouse brains. The experiments being perfromed will directly test the effects of alterations in neural circuitry in various animal models of Parkinson’s Disease.

2. Background Information and Objective: The NIH is an agency of the Department of

Health and Human Services and the Federal focal point for health research. Its mission is to seek fundamental knowledge about the nature and behavior of living systems and apply that knowledge to enhance health, lengthen life, and reduce illness and disability.

The National Institute for Neurological Disorders and Stroke (NINDS), which is an Institute within the NIH, seeks to reduce the burden of neurological disease through supporting and conducting basic, translational, and clinical research on the healthy and diseased nervous system, fostering the training of investigators in the basic and clinical neurosciences, and seeking better understanding, diagnosis, treatment, and prevention of neurological disorders.

The NINDS Cellular Neurophysiology Section is conducting research into how basal ganglia and midbrain dopamine circuits are altered in animal models of Parkinson’s Disease. To generate the Parkinson’s disease model, NINDS will express alpha synuclein in midbrain dopamine neurons; alpha synuclein is a protein that has been associated with cell death among dopamine neurons. This injection process will involve performing intracranial injections in mice. To precisely target the midbrain dopamine neurons through manual use of an injection needle requires substantial experience and can occur with variable success between animals. However, using a robot guided injection method will allow NINDS to more consistently target its brain structures of interest. Therefore, this is an essential piece of equipment for NINDS’ experiments.

3. Product Information:

• Brand Name of Product: Drill & Microinjection Robot (Mouse) including Drill Autostop Function

• Generic Name of Product: Automated stereotaxic injection system

• Product Description: Allows for automated drilling and precise injections into mouse brain tissue without tool exchange

• Manufacturer: Neurostar GmbH - Kähnerweg 1, Tübingen, 72072, Germany

4. Salient characteristics:

The system must equal or exceed the following salient physical, functional, and performance characteristics:

• Interconnected automated drill and microinjection robot (mouse), which includes:

o Robot stereotaxic system with atlas integration o Motorized drill & microinjection module o Full control by the robot stereotaxic software o Full integrated stereotaxic atlas o Zeroing at Lambda/Bregma, correction for tilt errors o Individual atlas scaling o Drill autostop function o Automated drilling procedure without damaging the underlying brain tissue o High accuracy positioning of Burr Hole with depth control o No requirement for tool exchange after drilling o Use of Bregma-related coordinates o User defined injection rates o Real-time atlas-based visualization of syringe tip and injection sites o Compatibility with Microsoft Windows 10

• Drill automated craniotomy function

• Automated synchronization device for cannula and drill bit o Must allow automated synchronization between the drill and syringe o Must allow for optical detection of the drill bit and the syringe needle

• Software integration and holder o Must allow for synchronized Drill/Injection/Cannula implantation procedure o Must provide real-time atlas-based visualization of cannula tip o Must include holder for fiber optic ferrules

• Remote Control for robot stereotaxic

• Mouse heating pad with integrated wireless closed-loop controller o Full integration in the robot stereotaxic control software o Real-time display of temperature, heating state, running state o High precision temperature control, resolution of 0.1℃, set range of 20-

45℃

• Mouse Gas Anesthesia Head Holder

• Non-Rupture Ear Bars (Mouse) o 60° Non-Rupture tip

5. Quantity: One system, with all required components

6. Delivery Terms: Contractor shall deliver all equipment within 30 days after receipt of order to NIH Building 35, Room 3C-1002, 35 Convent Drive, Bethesda, MD 20892- 3700. This shall be done in coordination with the Contracting Officer’s Representative (COR). Delivery must be FOB destination and include inside delivery. Contractor must also conduct a virtual session with Government personnel within two weeks of delivery to provide step-by-step instructions for equipment assembly.

7. Warranty Terms: Contractor shall warrant its equipment against defects in material, fabrication, or workmanship for a minimum of 12 months from the date the Government accepts delivery of the items.