Attachment 1- Purchase Description.pdf

PURCHASE DESCRIPTION

1.0 TITLE

LI-COR Biotech: Odyssey M Chemi/Fluor Imaging Bundle Standard

2.0 BACKGROUND

The National Institute of Health, National Cancer Institute/Center for Cancer Research/Laboratory of Human Carcinogenesis requires a new imaging system to replace our old, outdated, and failing protein imager currently in the laboratory branch. This imaging system supports all our on-going and future intramural research projects and initiatives. The Odyssey M is a versatile imaging system with simplified workflows that will be shared lab and division wide. Odyssey M provides a multitude of applications supporting our research projects that include fluorescent and colorimetric Western blots, immunocytochemistry (the In-Cell Western and the On-Cell Western), Enzyme-Linked Immunosorbent Assays, protein and nucleic acid arrays, high-resolution fluorescent and Red/Green/Blue tissue section analysis, fluorescent and colorimetric protein gel detection, nucleic acid detection, and non-isotopic Electrophoretic Mobility Shift Assays.

3.0 OBJECTIVES

Licor Odyssey M is a protein and nucleic acid imaging system with ease-of-use and flexibility that ensures effortless imaging for quantitative immunoassays. The objective of this acquisition is to provide scientists, fellows, and students in the lab branch as well as our Center for Cancer Research colleagues with an updated protein imaging system that allows for creative and innovative protein applications. This system will be used for near infrared and visible fluorescence, trans and epi illumination, and luminescence detection protein experiments performed in the laboratory. Odyssey M has all these applications in a single imaging instrument accommodated by our laboratory space allocation and budget.

4.0 TECHNICAL REQUIREMENTS

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS

The Odyssey M is a necessary and valuable instrument for protein analysis that meets and exceeds the minimum requirements in the laboratory. The Odyssey M system scans fluorescent and chemiluminescent protein and has other assay screening capabilities like in-cell westerns that would add detection tools for our teams to use for more accurate target analyte analysis. The software package included for qualitative analysis is found to be superior to other companies we have tested. This system provides everything that our current failing imaging system offers but also allows detection of NIR Fluorescence signals on gel, membrane, and slides. These advanced technological features would enable us to detect multiple targets at one single step, which will significantly reduce noise and variability of experimental results. The system dimensions fit into the lab space allocation. Additionally, the versatility of the Odyssey M Imaging System is a unique feature and of significant benefit to researchers. With a single instrument, researchers can complete a multitude of applications that include fluorescent and colorimetric Western blots, immunocytochemistry (the In-Cell Western and the On-Cell Western), ELISAs, protein and nucleic acid arrays, high-resolution fluorescent and RGB tissue section analysis, fluorescent and colorimetric protein gel detection, nucleic acid detection, and non-isotopic EMSAs.

Moreover, the Odyssey M Imaging System was developed and optimized to detect LICORbio’s IRDye infrared dyes and VRDye visible dyes with the highest accuracy and sensitivity. It was not allowed to compromise performance in order to access other applications. It is unique in its combination of ultimate sensitivity and quantitative data output. Especially at the 800 nm range, no other vendors’ systems can achieve the same sensitivity.

The following product features/characteristics are required for this requirement:

Hardware Specifications:

• Four solid state laser diodes emitting at 488 nm +5nm, 520 nm +5 nm, 685 nm +5 nm, and 785 nm + 5 nm respectively must be the means of excitation for the fluorescent labels.

• Two sets of RGB LEDs must be the source for trans and epi illumination.

• Fluorescence detection must be based upon line scanning utilizing scientific Complementary

Metal-Oxide-Semiconductors (sCMOS).

• Laser lifetime must be a minimum of 20,000 hours of operation to keep maintenance operational costs extremely low.

• Luminescence detection utilizing a CCD must be offered as an option.

• All functionalities including fluorescence detection, luminescence detection, and trans and epi illumination must be housed within a single imaging system, not multiple pieces of equipment combined to obtain all functionality.

• Dynamic range of the detection system must be at least 6 orders of magnitude in a single acquisition.

• Laser/Microscope must be integrated into a precision scanning mechanism capable of scanning at resolutions of 5 to 100 m.

• The data output must be 16 Bit Floating TIFF file format for each channel of detection.

• The system must utilize a sealed, flat-bed scanning surface to accommodate varying sample media including membranes, multi-well plates, gels, and slides.

Key Application Specifications:

The Odyssey M Imaging System will be utilized for creative and innovative applications developed by our end users. However, there are some minimally required key applications supported by LICORbio’s expert technical support staff that a single instrument, in this case the Odyssey M Imaging System, must be capable of performing the following:

• Up to 4-color quantitative and highly sensitive membrane-based Western blot analysis with signals being detected at 530 nm, 590 nm, 720 nm, and 820 nm for ultimate sensitivity in a single acquisition.

• Detection of membrane-based colorimetric Western blots utilizing epi-illumination.

• Up to 4-color detection of In-Gel-Western assays for immediate detection of proteins prior to blotting.

• Highly sensitive and quantitative detection of fluorescent protein stains including Coomassie Blue, Pro-Q Diamond, Pro-Q Emerald, SYPRO Ruby, and SYPRO Orange.

• Highly sensitive detection of colorimetric protein gel stains including Coomassie Blue and Silver

Stain utilizing transillumination.

• Fluorescent detection of nucleic acid stains including ethidium bromide, GelRed, and SYBR Safe.

• EMSA detection and quantitative signal analysis using NIR-labeled DNA oligos must be possible in 2 channels simultaneously without the time consuming need to disassemble the gel/plate sandwich.

• Quantitative detection of target molecules in cell culture grown in 6 – 384 well microtiter plates.

Quantitative analysis must be possible for all target molecules or of surface-exposed target molecules. Normalization of the target molecule by the cell number must be possible by the simultaneous and quantitative detection of a second target protein, nucleic acids, or a nonspecific cell stain.

• Quantitative detection of fluorescent proteins in both the visible and near-infrared spectra must be possible.

• Quantitative and highly sensitive fluorescent detection of protein arrays with a spot size >150 m on membranes or nitrocellulose coated glass slides must be possible.

• Fluorescent and colorimetric tissue section analysis must be possible at a resolution of 5 m.

• The sample imaging area must be large enough to accommodate the imaging of up to 4 microtiter plates or 12 glass slides.

The versatility of the Odyssey M Imaging System is a unique feature and of significant benefit to researchers. With a single instrument, researchers can complete a multitude of applications that include fluorescent and colorimetric Western blots, immunocytochemistry (the In-Cell Western and the On-Cell Western), ELISAs, protein and nucleic acid arrays, high-resolution fluorescent and RGB tissue section analysis, fluorescent and colorimetric protein gel detection, nucleic acid detection, and non-isotopic EMSAs.

Reagent Specifications

• Available reagents must include fluorescent IRDye infrared dyes and VRDye visible dyes for labeling antibodies, antibody fragments, peptides, proteins, oligonucleotides, and DNA fragments for emissions higher than 700 nm.

• Reagent offerings should include NIR solutions for the detection of HRP-conjugated antibodies and biotin-conjugated antibodies.

• Reagent offerings should include both NIR and visible membrane stains for the detection of total protein in a sample.

• IRDye infrared dyes must be available for direct detection of samples on membranes, gels, glass and plastic support.

• The system must be able to scan for 2 different IRDye infrared dyes with emissions separated by at least 100 nm to eliminate spectral overlaps.

• IRDye fluorophores must be very stable over several months and should provide the option to be rescanned with equivalent results.

Service and Support

• The system purchase should include installation and training, a one-year warranty for parts and labor, and an option to purchase support contract within one year of system purchase date.

• The manufacturer shall provide highly knowledgeable, professional and readily accessible PhD

Field Application Scientists (FAS) for on-site and / or remote training, servicing and hardware, software and application support of all key applications.

• The FAS team also provides complimentary assay and experimental design consultation for instrument owners and users.

• The manufacturer shall provide highly knowledgeable, professional and readily accessible Technical Support personnel for remote hardware, software and application support.

• The manufacturer shall provide highly knowledgeable, professional and readily accessible Service

Technicians for on-site and / or remote servicing of instrumentation.

The manufacturer shall supply customers with up-to-date protocols and application guides.

4.2 TRAINING

Within 14 business days after installation, 1 day(s) of onsite training shall be provided for at least 10 users of the equipment on how to use and maintain the instrument and associated software. All training expenses, including materials and/or travel expenses, shall be included. LI-COR Biotech includes unlimited remote Training on New User hardware, software, and application usage with an Expert Coach.

In addition, they offer remote Advanced Training on personalized advanced software and application usage with an Expert Coach.

4.3 SPECIAL ORDER REQUIREMENTS

Scientific software for this system is provided by the vendor. No cluster processing is needed. A Windows 11 computer is included, on premise (not cloud). Software would be installed on provided computer configured by NIH CIT as SPE account managed by Marshonna Forgues. No SaaS required (cloud).

5.0 TYPE OF ORDER

This is a Firm Fixed-Price Purchase Order.

7.0 DELIVERY / INSTALLATION

No facility renovation is required for this equipment acquisition.

Delivery shall be within 30-45 business days of purchase order award. All shipping/handling (including F.O.B. Destination) and delivery/installation fees shall be included. Upon shipment, the Contractor shall email the NCI Technical Point of Contact (TPOC), a shipping tracking number.

Upon delivery, the Contractor shall notify the NCI TPOC to schedule the installation date and time.

Installation shall occur within 15 business days of delivery, and shall be performed, or under the direct supervision of an Original Equipment Manufacturer (OEM) certified technician. Equipment shall be delivered and installed to the following address:

National Institutes of Health Laboratory of Human Carcinogenesis 37 Convent Drive, Bldg 37 Rm 3044 Bethesda, MD 20892-4258

7.2 TECHNICAL POINT OF CONTACT

All work delivered to the Technical Point of Contact (TPOC) will be deemed to have been accepted upon written confirmation of acceptance by the TPOC. The TPOC’s approval or revision to the work delivered shall be within the general scope of work for the Purchase Order.

Performance of work under this Purchase Order must be subject to the technical direction of the TPOC identified above. The term "technical direction" includes direction to the Contractor that fills in details and otherwise serves to ensure that tasks outlined in this requirements document are accomplished satisfactorily. Technical direction must be within the scope of this requirements document.

The TPOC does not have authority to issue technical direction that:

a. Constitutes a change of assignment or additional work outside of this requirements document

b. Constitutes a change as defined in the clause 52.212-4 (c) governing "Changes";

c. In any manner causes an increase or decrease in the contract price, or the time required for contract performance;

d. Changes any of the terms, conditions, or requirements of the contract;

e. Interferes with the Contractor's right to perform under the terms and conditions of the contract; or

f. Directs, supervises, or otherwise controls the actions of the Contractor's employees.

Technical direction may be oral or in writing. The TPOC shall confirm oral direction in writing within five business days, with a copy to the Contracting Officer. The Contractor shall proceed promptly with performance resulting from the technical direction issued by the TPOC. If, in the opinion of the Contractor, any direction of the TPOC, or their designee, falls within the limitations in (b), above, the Contractor shall immediately notify the Contracting Officer no later than the beginning of the next Government business day.

8.0 WARRANTY

The Contractor shall warrant all supplies furnished under this award against any defects in design, material, or manufacture for a period of at least 12-months from date of installation. The Contractor shall also warrant and imply that all items delivered as a result of this order are merchantable and fit for use for the particular purpose described in the order in accordance with FAR 52.212-4(o).

If a defect results in lost or decreased performance of supplies or services furnished under this order, the Contractor shall cover all parts, materials, labor, and/or travel expenses required to repair or replace the supplies at no cost to the Government.

Supplies which are repaired or replaced shall be warranted for the remainder of the initial warranty period or for 90-days – whichever is greater.

8.0 WARRANTY