Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this requirement is to acquire one (1) Evident Scientific, Inc.’s Olympus Automated VS200 Slide Scanner System to examine the circuits surrounding dopamine neurons located in the midbrain.

2. Background Information and Objective:

The National Institute of Neurological Disorders and Stroke (NINDS) is a part of the National Institutes of Health (NIH), conducting research into the causes, treatment, and prevention of neurological disorders and stroke. The NINDS mission is to seek fundamental knowledge about the brain and nervous system and to use that knowledge to reduce the burden of neurological disease.

The Cellular Neurophysiology Section (CNS) research in 12 focuses on circuit and cellular properties of dopaminergic neurons located in the midbrain. We are interested in the physiology of a subset of dopamine neuron located in the substantia nigra pars compacta (SNc) that undergo selective cell death in Parkinson’s patients. Specifically, we are interested in understanding how synaptic and intrinsic conductances differ among subpopulations dopamine neurons with the hope that this may provide insight to why SNc dopamine neurons are particularly vulnerable in Parkinson’s disease. Our goal is to identify novel physiological and genetic markers in single SNc dopamine neurons which may be useful in defining subpopulations of these neurons in a meaningful way.

The Cellular Neurophysiology Section requires a Evident Scientific, Inc.’s Automated VS200 Slide Scanner System microscopy to analyze mouse brain sections. The automated slide scanner allows CNS lab members to image up to 6 slides at a time with minimal human hours; maybe 15-20 minutes of initial setup, then the entire slide will be imaged overnight. Using a traditional microscope, it would take one of the CNS lab members ~1 full day to image all of the brain sections on 1 slide. With an automated slide scanner, 6 slides can be imaged overnight, and the decrease in time at the microscope allows lab members to be more efficient with their projects. Thus, the automated slide scanner will significantly increase the CN’s productivity.

3. Generic Name of Product:

Automated slide scanner

4. Purchase Description:

The NINDS mission is to seek fundamental knowledge about the brain and nervous system and to use that knowledge to reduce the burden of neurological disease for all people.

The NINDS CNS focuses on circuit and cellular properties of dopaminergic neurons located in the midbrain and is interested in the physiology of a subset of dopamine neuron located in the substantia nigra pars compacta (SNc) that undergo selective cell death in Parkinson’s patients. Specifically, the laboratory’s staff are interested in understanding how synaptic and intrinsic conductance differs among subpopulations dopamine neurons with the hope that this may provide insight to why SNc dopamine neurons are particularly vulnerable in Parkinson’s disease.

To identify novel physiological and genetic markers in single SNc dopamine neurons which may be useful in defining subpopulations of these neurons in a meaningful way by conducting microscopy to analyze mouse brain sections, the brand-name item that meets NINDS need is Evident Scientific, Inc.’s Automated VS200 Slide Scanner System.

The significant, essential physical, functional, performance, or material element(s) that are the Government’s minimum acceptable standards for an equivalent system to meet the government’s objective are as follows:

An equivalent automated slide scanner system shall:

- have microscope objectives that include the following objectives with its listed magnifications:

• Objective 1, 2x ACH

• Objective 2, 20x XAPO

• Objective 3, 10X UPLXAPO (OFN26.5, NA0.40, WD3.1mm)

• Objective 4, 40X UPLXAPO (OFN26.5, NA0.95, WD0.18mm, W/CC)

• Objective 5, 4x UPLXAPO

• Objective 6, 40X UPLSAPO40XS (U Plan S-Apo N 40X Si Oil Objective, NA

1.25, WD 0.30MM,W/CC, BFP1)

- have fluorescence cubes with the following filter sets:

Filter set 1, mCherry Filter set that includes the following filters, 578/21X/BS596/641/75M

Filter set 2, ET-ECFP Filter Set 436/20X, BS455, 480/40M w/BX3 cube.

Filter set 3, ET-EYFP Filter Set 500/20X, BS515, 535/30M w/BX3 cube.

Filter set 4, this is a custom filter set that shall contain filters that can visualize Penta.

DA, FITC, TRITC, Cy5, Cy7 Sedat set, 25mm filters, no cube.

- have a color camera to image the slides that is an ORCA-Fusion BT back-thinned Gen- IIIsCMOS camera to image up to 30 frames per second at full-frame.

- Have a light source in order to illuminate samples with the equivalent X-Cite XYLIS II, XT730S (365nm) specifications.

- have a workstation that includes an integrated computer, monitor and software. The software should perform 3D deconvolution that takes advantage of deep learning algorithms. The software shall perform automatic focus mapping, automatic scanning, automatic stitching, Z-stack imaging, and extended focus imaging (EFI). The software should use the following image formats: VSI, JPEG, TIFF, DICOM. The software shall also perform stepless zooming, zooming while scanning, make annotations, perform screen capture and have a slide loader control panel.

- The scanner system shall be warranted, and the Contractor shall warrant its equipment against defects in material, fabrication, or workmanship for a minimum of one (1) year from the date the Government accepts delivery of the item.

5. Salient characteristics The NINDS Cellular Neurophysiology Section requires one (1) slide scanner system that meets the following requirements:

The system must have:

The following objectives - 2x ACH and 20 X XAPO objectives, 4x UPLXAPO objectives, UPLXAPO10X air, UPLXAPO40X, UPLSAPO40XS

-an X-Cite Xylis light source

-the Sedat penta-cube filter set (DAPI, FITC, TRITC, CY5, CY7)

-additional filter cubes for CFP, YFP, mCherry

-automatic oil dispenser

-Hamamatsu Orca Fusion Camera

-1X6 slide tray for standard slides

-1X2 slide tray for 4X3 slides

The system must include acquisition software as well as a computer and monitor.

6. Quantity:

One (1) slide scanner along with ORCA-Fusion BT back-thinned Gen-III sCMOS CAMERA, acquisition computer and monitor as well as associated software and hardware, and user manual are required. This should also have a 1 year warranty.

7. Delivery Date:

In coordination with the COR, the contractor shall deliver and install the required equipment within 120 days after receipt of order. Delivery must be FOB Destination and must include inside installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday.

8. Period of Performance: Not applicable

9. Option(s): Not applicable