Attachment 1 DNA and RNA Sequencing Requirements.pdf
PDF 171 KB Posted
- Attached to
- Genomic Resequencing Federal contract opportunity
- Solicitation number
- 12805B22Q0418
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Sol_12805B22Q0418_Amd_0002.pdf | ||
| Attachment 1 Revision 1 DNA and RNA Sequencing Requirements.pdf | ||
| Sol_12805B22Q0418_Amd_0001.pdf | ||
| Solicitation 12805B22Q0418.pdf |
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Requirements Document
Project: Whole genome resequencing and RNAseq Requirement
GENERAL INFORMATION
1.0 Background and Purpose: Whole genome resequencing for 200 bulked mutant mapping populations is requested to identify genes responsible for dwarf, altered high temperature tolerance, and male sterile mutants discovered by two CRIS projects (3096-21000-021-00D and 3096- 21000-022-00D). These genes are critical to develop high yield and stress tolerant sorghum hybrids. RNAseq for 300 samples is requested to characterize the mechanism of panicle development and yield formation. This service also provides a wide range of targets to improve sorghum.
2.0 Definitions: N/A
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks: To deliver high quality DNA sequencing and RNA sequencing data.
4.0 Deliverable / Schedule:
o Promptly when DNA and RNA samples are submitted.
5.0 Government Furnished: High quality genome DNA and total RNA
6.0 Travel: No travel needed
7.0 Contractor’s Key Personnel: Sale’s rep and project manager
8.0 Security Requirements: None
9.0 Data Rights: Confidential to ARS. The sequencing data should not released to third party.
10.0 Section 508 – Electronic and Information Technology Standards: Operational software maintained to operate under current Windows operating system.
11.0 Performance Requirement Measures:
Whole genome resequencing: Over 10 Gb high quality DNA sequencing data per sample.
RNAseq: >2 million reads of 150 bp sequencing length.
6.0 Travel: No travel needed
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