US_SEQUEL_PRICE_LIST_JAN_23_2017.pdf

U.S. SEQUEL PRICE LIST (USD)

Part Number Product Name / Description US List Price

(USD)

100-426-900 Sequel™ System $360,000

Sequel System including Instrument, Primary Analysis compute, Sequel Software (Instrument Control, Signal Processing, Base Calling, Quality Assessment, Alignment, Assembly, Visualization) and Installation Kit. Includes 12-month comprehensive warranty subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

101-052-900 Sequel Consumable Starter Kit 2.0 $18,176

Starter bundle that provide a sampling of consumables to start running the Sequel System including Template Preparation including Sequencing Primer v3, Sequel Binding 2.0 and Sequencing Kit 2.0, MagBead Kit v2, controls, SMRT®Cell 1M v2, tips, SMRTbell Clean Up Columns and plates required to run ~16 SMRT® Cell 1Ms v2. This Starter Bundle is designed for new customers only.

Part Number Product Name / Description US List Price

(USD)

100-609-000 UPS, 208V, 5000VA, 50-60 Hz, UL (Sequel™ System) $7,000 Instrument Grade UPS for use in the USA and Canada with Sequel System. 208 V, 50/60 Hz. The North American UPS purchased from Pacbio is pre-configured and will be installed at the time of the Instrument Installation.

Part Number Product Name / Description US List Price

(USD)

100-991-900 SMRTbell™ Template Prep Kit 1.0-SPv3 $670

The SMRTbell Template Prep Kit 1.0-SPv3 contains reagent supplies to perform SMRTbell library preparations of primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb.

Reagent quantities support 10 library preparations. This kit includes both Sequencing Primer v2 for use with PacBio RS II and Sequencing Primer v3 for use with Sequel System. Each primer supports 10 primer annealing reactions.

100-970-100 Sequencing Primer v3 $20 Sequencing Primer v3 is used to anneal to SMRTbell™ templates as an alternate to Sequencing Primer v2. Sequencing Primer v3 is compatible with SMRTbell™ templates and should only be used for SMRTbell libraries for MagBead loading on Sequel System.

100-466-100 Barcoded Universal F/R Primers Plate - 96 $156 Barcoded Universal F/R Primers Plate - 96 is a 96-well plate containing barcoded primers. Each well on the plate contains a primer pair consisting of forward and reverse primers having the same barcode.

Each barcode is present in only one well for a total of 96 different barcodes on the plate. Each forward and reverse primer has a universal sequence on the 3’ end. Reagent quantities support a single use of each of the 96 primers pairs in library preparations.

100-514-900 SMRTbell™ Barcoded Adapter Complete Prep Kit - 96 $1,747 The SMRTbell Barcoded Adapter Complete Prep Kit- 96 contains both SMRTbell Barcoded Adapter Prep Kit and Barcoded Adapter Plate - 96. Reagent quantities support 96 complete Barcoded Adapter End Repair and Ligation reactions. Use with SMRTbell Damage Repair Kit to complete template preparation and yield primer-annealed SMRTbell libraries.

100-929-600 SMRTbell™ Clean Up Column Kit $130 The SMRTbell™ Clean Up Column Kit contains sufficient columns and reagents to support the processing of 5 polymerase binding reactions. Bundle components include:

SMRTbell™ Clean Up Columns (5) SMRTbell™ Column Conditioning Buffer Kit

Instrument and Consumable Starter Pack

Accessory Third Party Equipment – Optional

Reagent Kits and SMRT® Cells

100-992-200 SMRTbell™ Damage Repair Kit-SPv3 $956

The SMRTbell Damage Repair Kit contains reagent supplies to perform DNA Damage Repair, Exonuclease III and VII treatments, and primer- annealing of SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities support 10 library preparations. To be used with SMRTbell Barcoded Adapter Complete Prep Kit to generate primer-annealed SMRTbell libraries ready for polymerase binding or to be used independently for extra DNA Damage Repair of template. This kit includes both Sequencing Primer v2 for use with PacBio RS II and Sequencing Primer v3 for use with Sequel System. Each primer supports 10 primer annealing reactions.

100-862-200 Sequel Binding Kit 2.0 $495

The Sequel Binding Kit 2.0 contains reagent supplies to bind prepared DNA template libraries to the Sequel Polymerase 2.0 in preparation for sequencing on the Sequel System. The result is a DNA polymerase/template complex. Sequel Polymerase 2.0 should be used only with Sequel Sequencing Kit

2.0. Reagent quantities support 24 binding reactions.

100-715-300 Loading Cleanup Bead Kit (10 rxn) $624

The Loading Cleanup Bead Kit (10 rxn) contains reagents to remove excess polymerase from a polymerase binding reaction. The Loading Cleanup Beads are only for diffusion loaded samples onto the Sequel SMRT Cell 1M. Reagent quantities support cleaning up 10 polymerase binding reactions.

101-011-000 Sequel Sequencing Kit 2.0 $825 The Sequel Sequencing Kit 2.0 (8rxn) contains a sequencing reagent plate with Sequel Sequencing chemistry 2.0 and a tube of Sequel Oxygen Scavenging (OS) Enzyme for SMRT Sequencing on the Sequel System. Reagent quantities support 8 sequencing reactions. The Sequel Sequencing Kit 2.0 to be used with a Sequel SMRT® Cell 1M v2. Sequel Sequencing Kit 2.0 is only compatible with templates bound to Sequel DNA Polymerase 2.0.

101-053-000 Sequel Sequencing Kit 2.0 Bundle (5 pack) (5 pack) $4,312 The Sequel Sequencing 2.0 Bundle (5 pack) contains reagent and disposable supplies for 40 reactions of SMRT Sequencing. It contains 5 Sequel Sequencing Kits 2.0, 1 Sequel SMRT Cell Oil pack, and 1 Tube Septa pack.

101-008-000 Sequel™ SMRT® Cell 1M v2 Tray (4 cells) $2,200

The Sequel SMRT Cell 1M v2 Tray (4 cells) are nanofabricated consumables each patterned with 1,000,000 wells called zero-mode waveguides (ZMWs). They are packaged together in a streamlined 4 SMRT Cell format. SMRT Cell 1M v2 is compatible with Sequel Binding Kit 2.0 and Sequel Sequencing Kit 2.0. One SMRT Cell 1M is utilized in each sequencing reaction, and experiments can be run on a single or in batch mode, giving your projects a greater level of flexibility.

100-621-300 Sequel™ SMRT® Cell Oil (5 pack) $156 The Sequel SMRT Cell Oil contains 5 reagent tubes for SMRT Sequencing on the Sequel System (used with the Sequel Sequencing Kit). Reagent quantities support 5 sequencing runs.

100-265-900 AMPure® PB (5ml) $306 AMPure PB magnetic beads are specifically formulated for Pacific Biosciences’ SMRT Sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow. Reagent quantity supplied at 5 mL volume.

100-676-500 MagBead Kit v2 $1,196 The MagBead Kit v2 contains reagent supplies to capture DNA polymerase and template complex for loading onto the PacBio® systems. Kit components include: MagBeads, MagBead Binding Buffer Kit v2 and Wash Buffer kit v2.

100-125-900 MagBeads $1,000 The MagBeads contains 2.88 mL of MagBeads to capture DNA polymerase and template complex for loading onto the PacBio® systems. MagBeads are to be used with MagBead Binding and Wash Buffer Kits.

101-046-400 MagBead Binding Buffer Kit v2 $98

The MagBead Binding Buffer Kit v2 is used with the MagBead Wash Buffer Kit and MagBeads using the Pacific Biosciences Binding Calculator to bind the DNA/Polymerase complex.

101-046-500 MagBead Wash Buffer Kit v2 $98

The MagBead Wash Buffer Kit v2 is used with the MagBead Binding Buffer Kit and MagBeads using the Pacific Biosciences Binding Calculator to bind the DNA/Polymerase complex.

Labware

Part Number Product Name / Description US List Price

(USD)

100-667-700 Tube Septa (10 septa) $31 One bag of 10 tube septa approved for evaporation control of Oxygen Scavenging (OS) Enzyme and SMRT Cell Oil tubes on the PacBio® systems.

100-667-400 Sequel™ Sample Plate Foil (100 Seals) $156 One case of 100 foil seals approved for evaporation and environmental control contained in the Sequel Sample Plate. The foil seals are heat-treated to cover the Sequel Sample Plate.

000-448-888 Sample Plate (50 plates) $400 One case of 96-well PCR plates contains 50 plates approved for sample placement on the PacBio® systems.

100-667-500 Sequel™ Mixing Plate (120 Pack) $1,456 One case of 96-well deep-well plates contains 120 plates approved for reagent and sample mixing on the Sequel System.

100-667-600 Sequel™ Pipet Tips (40 Pack) $1,560 One case of sterile filter tips approved for robotic liquid handling on the Sequel System. Each case contains 40 racks of 96 tips each.

Training

Part Number Product Name / Description US List Price

(USD)

100-125-100 On-Site System Training (3 Days) $5,200 On-site instruction of up to three (3) participants at the customer's location by a Pacific Biosciences Field Applications Specialist (FAS). Includes training kit and FAS travel-related expenses. Training includes Assay, Software and Bioinformatics.

Standard Service Contracts

(USD)

100-715-500 Comprehensive Sequel™ Service Contract with Insight $30,000

Covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

100-715-600 Comprehensive Sequel™ Service Contract without Insight $35,000

Covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

100-715-700 Basic Sequel™ Service Contract with Insight $25,000

Covers parts and labor required for the repair of an installed system. Subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

100-715-800 Basic Sequel™ Service Contract without Insight $30,000

Covers parts and labor required for the repair of an installed system. Subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

Certain products or portions thereof are manufactured and sold under license from GE Healthcare under patents US 7,056,670, US 7,052,839, US 7,033,762, US 7,041,812, US 7,223,541, US 7,244,566, US 7,560,254, US 7,393,640, and US 7,264,934 and other pending and foreign patent applications. Certain products or portions thereof are manufactured and sold under license from GE Healthcare under its exclusive license from Carnegie Mellon University under U.S. Patent Numbers 6,008,373, 6,130,094, 6,479,303, 6,545,164, 6,673,943 and related patents. Use of such products or portions thereof for purposes other than performing research or research services may require a commercial sublicense from GE Healthcare Biosciences Corp. See applicable product insert for details.

For Research Use Only. Not for use in diagnostic procedures. © Copyright 2010 - 2016, Pacific Biosciences of California, Inc. All rights reserved. Information in this document is subject to change without notice.

Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences products and/or third party products. Please refer to the applicable Pacific Biosciences Terms and Conditions and the applicable license at terms at http://www.pacificbiosciences.com/licenses.html. Pacific Biosciences, the Pacific Biosciences logo, PacBio SMRT, SMRTbell, Iso-Seq, and Sequel are trademarks of Pacific Biosciences in the US and/or certain other countries. All other trademarks are the sole property of their respective owners.

Contact Information Pacific Biosciences 1305 O'Brien Dr. Menlo Park, CA 94025 United States www.pacificbiosciences.com Sales and Support: +1 (877) 920 7222 Email: sales@pacb.com

Quotation and Ordering Information Pacific Biosciences 1305 O'Brien Dr. Menlo Park, CA 94025 United States www.pacificbiosciences.com Sales and Support: +1 (877) 920 7222 Email: orders@pacb.com

US Sequel Jan 1