RFQ Attachment 1_Purchase Description.pdf

FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Title: Integrated Cage Promethion Metabolic System (or Equal).

1. Statement of Need and Purpose: The purpose of this acquisition is to acquire a metabolic and behavioral phenotyping system that enables the tight synchronization of metabolic data with behavioral events. The system must integrate multiple physiological measurements, including of energy expenditure, RQ, VO2, VCO2, animal activity, food/water consumption, and body temperature in mice for circadian and aging studies at the National Institute on Aging (NIA) Intramural Research Program on the Johns Hopkins Bayview campus.

2. Background Information and Objective: Causative factors governing health deterioration as we grow older remain poorly understood. Even among genetically identical mice, there is considerable variability in health status, aging trajectory, and lifespan. Integrating multiple metabolic and behavioral outputs are indispensable for identifying biological indicators that precede the onset of age-related diseases. The incorporation of the system represents a significant enhancement to the NIA existing Comprehensive Lab Animal Monitoring System - CLAMS and will provide versatility and superior capacity to control environmental light and temperature while integrating multiple metabolic and behavioral variables with minimal disturbances to the animals. Of particular significance is its capacity to incorporate telemetry for assessing body temperature and regulating exercise as a lifestyle intervention, alongside different dietary regimens. This expansion broadens the scope of studies conducted at NIA. Furthermore, the system's broad range of light and ambient temperature settings will enable NIA researchers to conduct metabolic and aging studies in more natural conditions, such as thermoneutrality.

Under these conditions, the body is not metabolically stressed to maintain the body temperature within physiological range, offering more accurate reflection of the aging process and the effect of the interventions to promote healthier longevity.

This system must contain customizable features including programmable light intensity and a broad temperature range (3ºC to 50ºC) using a digital display. It must be user-friendly design incorporates pull-out shelving for easy install and access to rodents during experiments, and effortless cleaning.

With adherence to the standard of best animal care practice, this system must be equipped with an environmental cabinet event registry. The system must record user interactions such as door openings and closings, fluctuations in cabinet light and temperature, system errors, and operator annotations. It must uphold security protocols, user administration and pass-protected login functionalities to prevent unauthorized access.

Furthermore, this comprehensive system must integrate advanced monitoring capabilities to track energy expenditure, gas exchange (O2, CO2 and H2O), body temperature through telemetry and a remarkable 0.002 g resolution in body mass, ingestive behavior, and water consumption. It must record voluntary exercise via a running wheel count as well as home cage activity XYZ. It must be capable of programmable wheel stop, which enables accurate assessment of exercise's impact on metabolism. Data logging and remote access must be seamlessly integrated, allowing for real-time monitoring and analysis.

The system's web-based software must enable precise programming tailored to various research goals, including the examination of exercise and dietary interventions (such as caloric or time restriction, high-fat diets), as well as the impact of sex and genetic backgrounds on aging studies in rodents.

3. Generic Name of Product: Metabolic cages for rodents.

4. Purchase Description: Promethion Core System (or equal)

HARDWARE:

CGF-16-NA (x2)

16 cage- Triple gas analyzer (O2, CO2, H2O) and flow regulator, flow rates up to 2.0 L/minute (4.0 L/min with KIT-PUMP-BOOST). Includes sub-sampling, internal temperature control, barometric pressure compensation, and software controlled automated drift correction. Includes internal data storage.

ACC-BASELINE-M (x4) Baseline container for mouse.

MAGCAGE-RESP-DA-WHEEL (x32)

Magnetic Respirometry Cage with dual access control and wheel - (37cm x 21cm) with (530 cm2) floor area with magnetic solid lid - includes respirometry manifold, 2 magnetic hole covers, 2 access control hole covers, filter, and tubing, and 1 spare cage tub. Compatible with two MM2s, 2 AC2s, and 1 wheel.

ACC-MM-1-FH-3 (x32) Food hopper for Cage 3721, stainless steel.

AC-2 (x64)

Access Control Unit (mouse size). Not to be used in combination with optogenetic tethers.

ACC-MM-1-WH-AC (x32) Water bottle, sipper tube, bracket, and housing for cage 3721. Compatible with AC-2 for water access control.

MM-2 Product (x64) Magnetic mounted Precision mass monitoring unit, 0 - 750g, 2mg resolution, hook version, for use with magnetic cage lids.

CC-2 Product (x32) Cage controller data interface for mass monitoring sensors, running wheels, BXY and BZ position sensors using power.

BXYZ-M (x32) Three-dimensional IR beam break array for position detection and rearing information for cage 3721. Includes mounting base.

WHEEL-M (x32) In-Cage wheel and monitoring device for voluntary exercise wheel counts, 4.5" (11.43cm) diameter for mice and similar small animals. Includes carbon fiber reed switch.

ESA-1 Product (x2) Environmental Sensor Array for recording ambient temperature, light, RH, sound, BP, noise, and motion data.

CAB-16-L-NA (x2) Temperature cabinet. Volume test room 1230L. Incl. regulation of temperature and light.

Double door cabinet. Accommodates up to 16 cages. CGF mounts on left side. 120 V, 60 Hz, with plug type B.

ACC-TELEM-IM3-CTRL (x2) Telemetry accessory kit for integration with the System using IM3 standalone.

TELEM-S (x8)

Telemetry bases (set of 4) for temperature, heart rate, and activity. Includes telemeters (no battery required) and software to integrate with system. Designed for use inside of temperature cabinets.

SOFTWARE:

PRO-LIVE-NA (x1)

Software platform for data acquisition, real-time data analysis, and remote system monitoring. Includes computer with pre-installed software.

OTHER

• Onsite Installation

• Onsite Training

• Shipping

5. Salient characteristics

• High-resolution gas analyzers oxygen and carbon dioxide gas coupled with precise metabolic measurements.

• Comprehensive behavioral monitoring, including running, eating, and drinking activities.

• Compatibility with telemetry for body temperature tracking

• Control over lifestyle interventions, such as exercise and diets.

• Optimized design for compact footprint and simplifies access to animals.

• Real-time system monitoring and experiment analysis via Live software

• Live access to study and animal information for real-time decision-making

• Intuitive setup screen simplifies input of cage and device parameters.

• Animal-specific data input enables tracking and real-time cohort analysis.

• Access to transparent and traceable raw data for future analysis

• Continuous monitoring of system performance, animal welfare, and data quality

• Flexible real-time analysis and visualization of grouped data

• Templates streamline study setup and ensure consistency.

• One-click calibration ensures accurate respirometry measurements.

• Data export to CSV files or SVG graphs for simplified presentation

• Secure web-based access for remote experiment viewing.

• Precise temperature control and automated light intensity management, with an alarm system to alert for changes in the environmental conditions.

• Touch screen digital controller with integrated data memory.

• Accommodates 16 mouse cages with pull-out shelves for easy setup.

• Dedicated air and communication lines for each cage minimize errors.

6. Quantity: CGF-16-NA (x2), ACC-BASELINE-M (x4), MAGCAGE-RESP-DA-WHEEL (x32), ACC-MM-

1-FH-3 (x32), AC-2 (x64), ACC-MM-1-WH-AC (x32), MM-2 Product (x64), CC-2 Product (x32), BXYZ- M (x32), WHEEL-M (x32), ESA-1 Product (x2), CAB-16-L-NA (x2), ACC-TELEM-IM3-CTRL (x2), TELEM- S (x8)

7. Delivery Date: All items delivered within 14 weeks after receipt of the Purchase Order.