RFQ 36C24E26Q0072 0001_ Amendment SF30.pdf

5. PROJECT NUMBER (if applicable)

CODE 7. ADMINISTERED BY

2. AMENDMENT/MODIFICATION NUMBER

CODE

6. ISSUED BY

8. NAME AND ADDRESS OF CONTRACTOR

4. REQUISITION/PURCHASE REQ. NUMBER 3. EFFECTIVE DATE

9A. AMENDMENT OF SOLICITATION NUMBER

9B. DATED

PAGE OF PAGES

10A. MODIFICATION OF CONTRACT/ORDER NUMBER

10B. DATED

BPA NO. 1. CONTRACT ID CODE

FACILITY CODE CODE

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

E. IMPORTANT:

is extended,

(a) By completing Items 8 and 15, and returning __________ copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY is not extended.

12. ACCOUNTING AND APPROPRIATION DATA

(REV. 11/2016)

is required to sign this document and return ___________ copies to the issuing office. is not, A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER NO. IN ITEM 10A.

15C. DATE SIGNED

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER

Contractor

16C. DATE SIGNED

14. DESCRIPTION OF AMENDMENT/MODIFICATION

16B. UNITED STATES OF AMERICA

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME AND TITLE OF SIGNER 16A. NAME AND TITLE OF CONTRACTING OFFICER

15B. CONTRACTOR/OFFEROR

STANDARD FORM 30 PREVIOUS EDITION NOT USABLE

Prescribed by GSA - FAR (48 CFR) 53.243

(Type or print) (Type or print)

(Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

(Number, street, county, State and ZIP Code)

(If other than Item 6)

(Specify type of modification and authority)

(such as changes in paying office, appropriation date, etc.)

(If required)

(SEE ITEM 11)

(SEE ITEM 13)

(X)

CHECK

ONE

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS,

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

(Signature of person authorized to sign) (Signature of Contracting Officer)

240E

0001 07-28-2026

CCDF ARGWAL

00240E

Veterans Health Administration Regional Procurement Office East Building 32 Suite 1A1111 1010 Delafield Road Pittsburgh PA 15215-1802

00240E

Veterans Health Administration Regional Procurement Office East Building 32 Suite 1A1111 1010 Delafield Road Pittsburgh PA 15215-1802

To all Offerors/Bidders

36C24E26Q0072

X

X X

See CONTINUATION Page

X -01-

Amendment issued to provide question and answer responses to benefit all offerers/bidders. Extension for submission is not granted.

All other terms and conditions remain in effect.

Millicent Covert Contracting Officer

AMENDMENT FOR SOLICITATION 36C24E26Q0072

This solicitation is being amended to provide Question and Answers to all offerers/bidders.

QUESTION and ANSWERS

Q1: What sequencing depth (Gb) or number of reads per sample (M) is required for the RNA-seq component? If there is no specific target, may we recommend one?

A1: 6Gb, 150 million reads

Q2: What volume of plasma and whole blood will be provided per sample type? Additionally, will 40 separate samples be aliquoted for each project (i.e., dedicated plasma tubes for proteomics and for metabolomics), or will the same plasma sample be sent for both and need to be split at the facility?

A2: Blood will be collected in specialized tubes (one tube is specialized for proteomics and one for metabolomics) – they cannot be collected as one, and then later separated. Each subject will be given three tubes each: PAXgene blood tube will be mailed intact (zero processing), metabolomics blood sample will be centrifuged to collect plasma and placed into its dedicated Eppendorf tube and metabolomics blood sample will be centrifuged to collect plasma and placed into its dedicated Eppendorf tube. There will be 40 subjects for a total of 120 tubes (40 intact blood tubes for RNA, 40 metabolomics plasma samples, and 40 proteomics samples).

Q3: The pricing table (Item 0002) specifies DIA-based LC-MS/MS proteomics, but the Scope/Task 2 narrative specifies "Olink proteomics analysis." These are different technologies (untargeted mass spec vs. targeted antibody-based panels) requiring different vendor capabilities and pricing. Please confirm which platform is required—DIA-MS, Olink, or both. If Olink is required, please specify the panel (e.g., Olink Explore HT, Target 96 Inflammation, etc.).

A3: Thank you for pointing out the discrepancy. DIA-base proteomics is the platform required.

Extension for offers is not granted. Submission deadline remains as no later than Friday, July 31, 2026, at 11:59AM Eastern Time.

IMPORTANT: complete all instructions as noted in “Instructions to Offeror” within original solicitation. Additionally, please complete Blocks 15A – 15C AMENDMENT OF SOLICITATION document. Incomplete submissions will be considered non-responsive.

All offeror quotes for this solicitation must be received electronically through email no later than Friday, July 31, 2026, at 11:59AM Eastern Time. Offers shall be sent by email to Millicent Covert at millicent.covert@va.gov. Reference solicitation number 36C24E26Q0072 within subject line of the email. Any quotes received after the prescribed date/time shall be considered non-responsive and will not be considered for award.

mailto:millicent.covert@va.gov