QuantStudio PM_JOFOC_Redacted.pdf

Version 2.0

SINGLE SOURCE DETERMINATION USING

SIMPLIFIED ACQUISITION PROCEDURES (SAP)

FOR AN ACTION NOT EXCEEDING THE SIMPLIFIED ACQUISITION THRESHOLD

(AUTHORITY: FAR 13.106-1(b)(1))

1. Agency and contracting activity. Department of Commerce, NOAA Acquisition and Grants Office (AGO), Alaska Fisheries Science Center, and Auke Bay Laboratories

2. Description of supplies or services required to meet agency needs (including the estimated value).

The Auke Bay Laboratories Genetics Program uses a plethora of next-generation sequencing protocols, including but not limited to environmental DNA (eDNA), Genotyping-in-Thousands by sequencing (GT-seq), and restriction site-associated DNA sequencing (RAD-seq), many of which are processed on a Life Technologies /ThermoFisher QuantStudio instrument. Annual preventative maintenance on this instrument by highly-trained specialist engineers is crucial for continued production of high-quality, reproducible next- generation sequencing for fish species identification and genetic stock identification, which are crucial to our agency’s mission.

Life Technologies /ThermoFisher QuantStudio instrument. The service requirement must include full support within 72 hours for all parts failures due to normal wear and tear (parts, labor, travel), one preventative maintenance visit per year (parts, labor, travel), unlimited software and firmware updates, and unlimited telephone support. Preventative maintenance includes travel to and from the site, annual inspection, re-calibration, performance verification of subassembly and electro- mechanical components, and certification that the instrument is performing to specifications.

The required service must be compatible in all aspects (form, fit, and function) with existing systems or equipment and the source is uniquely qualified to meet the requirement. A recent internet search failed to identify any additional maintenance service providers for the Life Technologies / ThermoFisher QuantStudio instrument other than the manufacturer (Life Technologies/ThermoFisher). A search on GSA Advantage did not locate any maintenance services available for the instrument. Life Technologies/ThermoFisher is the only company that offers maintenance and repairs for the Life Technologies/ThermoFisher QuantStudio instrument, which is highly specialized scientific equipment.

Life Technologies/ThermoFisher is uniquely qualified and offers exceptionally experienced technical support and service.

Highly specialized instruments such as this instrument require consistently-timed annual maintenance and a rapid response to address malfunctions in order to benefit from the high quality results the instrument is designed to produce. The previous use of third-party vendors was deemed detrimental in maintaining our equipment due to inadequate response to service requests and lack of efficient and timely maintenance and repair of equipment. Having a direct source to Life Technologies/ThermoFisher, the sole source available for repairs and maintenance on this instrument, is in the Federal Government's best fiscal interest, and allows the Genetics Program to continue production of high-quality, reproducible next-generation sequencing for fish species identification and genetic stock identification in a timely and efficient process without instrument down-time.

Period of performance:

Base Year Period of Performance: 10/01/25 – 09/30/26 Option 1 Period of Performance: 10/01/26 – 09/30/27 Option 2 Period of Performance: 10/01/27 – 09/30/28 Option 3 Period of Performance: 10/01/28 – 09/30/29

Total estimated value, including base year and three option years, is .

Identification of the single source or the brand name to be solicited.

Life Technologies/ThermoFisher 5791 Van Allen Way Carlsbad, CA 92008 POC: @thermofisher.com

Supporting rationale. Only one source or brand name is reasonably available as detailed below:

Other - Only one source is capable - Only one source is capable of providing the services required at the level of quality required because the supplies or services are unique or highly specialized. Life Technologies/ThermoFisher is the manufacturer of the QuantStudio instrument and is the only company that services and provides supplies for this platform. The Life Technologies/ThermoFisher QuantStudio instrument is highly specialized scientific equipment used in our genetics laboratory. Any potential third-party providers will only support scheduling required services with the manufacturer (Life Technologies/ThermoFisher), and will not offer maintenance services or qualified engineers. Life Technologies/ThermoFisher is the only source for qualified engineers for their instruments and is deemed the only source for providing the required maintenance and services. They are the only source that will meet the Government's needs.

Market Research.

A recent internet search failed to identify any additional maintenance service source providers for the Life Technologies/ThermoFisher QuantStudio instrument other than the manufacturer (Life Technologies/ThermoFisher). A search on GSA Advantage did not locate any service or supplies for this instrument. SAM indicated this is a large business.

6. Technical/Requirements Representative Certification I certify that this requirement constitutes the Government’s minimum needs and the supporting data provided herein is accurate and complete to the best of my knowledge and belief.

Jackie Whittle Date Biological Science Technician III

Robert Foy, AFSC Director Date

7. Determination

I hereby determine that the circumstances of this action deem only one source is reasonably available. This determination is accurate and complete to the best of my knowledge and belief.

Date Contracting Officer

Jackie Whittle Date

WHITTLE.JACQUELI

NE.ANNE.14640172

Digitally signed by

WHITTLE.JACQUELINE.ANNE.146

4017255 Date: 2025.04.25 10:00:24 -08'00'

LANG.CHRISTIE.A

NNE.1156642300

Digitally signed by

LANG.CHRISTIE.ANNE.1156642

Date: 2025.08.05 15:25:52 -07'00' 8/5/25