Sol_140R4026R0009_Amd_0002.pdf

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PAGESOF

Contractor

The hour and date specified for receipt of Offers to sign this document and return

Mary B. Maestas

See continuation page

Bureau of Reclamation Upper Colorado Region Regional Office 125 South State Street, Room 8100 Salt Lake City UT 84138

Bureau of Reclamation Upper Colorado Region Albuquerque Area Office 555 Broadway Blvd NE, Suite 100 Albuquerque NM 87102

R40

R4Q

140R4026R0009

07/22/2026

07/22/2026

1 10

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Sponsored by GSA FAR (48 CFR) 53.110

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NSN 7540-01-152-8067

140R4026R0009/0002

The purpose of this Amendment is to update the

Statement of Work.. see below

Period of Performance: 10/01/2026 to

03/31/2032

140R4026R0009 RGSM Spawning and Egg Monitoring.

Amendment 2

The purpose of this amendment is to correct dates listed in the Statement of Work – pages 20-26 of the solicitation should be replaced with pages below. Updated dates are highlighted in yellow.

D. MRG RGSM EGG SPAWNING AND EGG MONITORING

Statement of Work FY 2027

1.0 Background

Desert rivers of the American Southwest are impacted by physical modifications resulting from construction of dams, reservoirs, and diversion structures which fragment river reaches, alter habitat, and change the flow regime.

Pelagic broadcast fish eggs are semi-buoyant, drifting with river currents downstream as they spawn in response to increases in flow that occur during spring runoff or other flood events (Platania and Altenbach 1998). The semi-buoyant nature of Pelagic spawning fish eggs consequently results in them drifting downstream which leads to the displacement of the eggs into reaches that are separated from upstream reaches where they originated.

Populations in upstream reaches may gradually decline and could disappear entirely if there is little retention of eggs and larval fish in upstream areas (Winston et al. 1991; Luttrell et al. 1999). In the Rio Grande, populations of Rio Grande Silvery Minnow (RGSM) in downstream reaches are threatened annually because of prolonged and extensive river drying in these areas. Drying tends to occur during irrigation season after the species spawns.

These dynamics have important demographic and genetic consequences for Rio Grande Silvery Minnow.

Captive breeding facilities have been established to facilitate propagation and rearing of silvery minnow from eggs.

Captive silvery minnow populations include City of Albuquerque BioPark, Southwestern Native Aquatic Resources and Recovery Center (formerly known as Dexter National Fish Hatchery), and New Mexico Interstate Stream Commission Los Lunas Silvery Minnow Refugium. Eggs are collected from the wild and transported to these facilities for rearing, propagation, and to be released back into the Middle Rio Grande after irrigation season.

Monitoring the reproductive effort of RGSM is required by the 2016 Final Biological and Conference Opinion for Bureau of Reclamation, Bureau of Indian Affairs, and Non-Federal Water Management and Maintenance Activities on the Middle Rio Grande, New Mexico (2016 MRG BO) (USFWS, 2016). Compliance under the 2016 MRG BO requires RGSM reproductive monitoring and assessment work funded by this contract. Specifically, Reasonable and Prudent Measures (RPMs) 5.1, 5.2, 5.3 and 7.1 within the 2016 MRG BO are addressed by the actions outlined within this contract proposal.

The outlined contract actions serve the public's interest by ensuring that management actions are conserving and protecting the endangered RGSM in the Middle Rio Grande (MRG). The general public benefits by the preservation of this federally listed species, and use of reproductive data to ensure and develop successful strategies for long-term recovery of this species. Statutory authority for this contract is provided by the Fish and Wildlife Coordination Act of 1934 (Public Law 85-624). In addition, this contract is to support legal compliance for Reclamation pursuant to the MRG Project Authority – authorized by the Federal Flood Control Acts of 1948 and 1950 (P.L. 80-858 and 81-516).

In addition, the Middle Rio Grande Endangered Species Collaborative Program’s 2006 Program Monitoring Plan, Appendix A Rio Grande Fish Community Monitoring references this egg/spawning monitoring and the use of number of eggs captured per unit time per volume water sampled as the metric used to monitor reproductive success for the Rio Grande silvery minnow (MRGESCP 2006).

2.0 Scope

Scope of the Requirement

The scope of this requirement is to collect data that will enable characterization of the timing, duration, and magnitude of RGSM reproduction in the Middle Rio Grande. This includes sampling the river in three locations during the spawning season to detect spawning events and to inform and to coordinate with the US Fish and Wildlife Service, City of Albuquerque BioPark, and other entities or parties who annually conduct egg collection operations separately from this project. Secondarily, this project could provide assistance for salvaging eggs for movement to other sections of river, and may provide eggs for propagation (e.g., Albuquerque BioPark, Southwestern Native ARRC ). This item is listed in detail in the Rio Grande Silvery Minnow Genetics Management and Propagation Plan, Production Goal #1): “Annual collection of a minimum of 500,000 eggs for captive rearing and subsequent stocking of Rio Grande silvery minnow in the Middle Rio Grande” (USFWS 2009). The Contractor may be requested to retain and provide egg samples as required for other program studies other than for purposes of rearing and breeding. This project also has an optional task, subject to Government approval to proceed, to utilize a Moore Egg Collector (MEC) boat when optimal conditions occur within the establish RGSM egg collecting timeframe.

Recipients of award issued under this Request for Proposal/Quote are required to obtain all necessary Federal and State authorizations and to provide a copy of those permits to Reclamation prior to performance of work.

This includes the appropriate research and enhancement permits from the U.S. Fish and Wildlife Service (USFWS), as required by the Endangered Species Act (see 16 U.S.C. 1531 et al.) and its implementing regulations (e.g., 50 CFR 13, 17.22, 17.32). Additionally, the New Mexico Department of Game and Fish (NMDGF) requires a permit for the taking of fish as specimens for scientific or propagation purposes in accordance with the New Mexico Statutory Authority of 1978 Chapter 17-3-29. Receipt of an award under this RFP/RFQ does not guarantee issuance of the necessary permits or mandate a timeframe for permit issuance. The recipient must abide by the terms of the USFWS and NMDGF permits, including crew chief(s) and all members of the monitoring crews. The recipient must be able to secure federal and state collecting permits prior to conducting this work. Data collection is expected to begin in spring of 2027.

3.0 Objectives

3.1 Monitor spawning of RGSM in the Rio Grande by collecting data that will enable characterization of the timing, duration, and magnitude of RGSM spawning in the Middle Rio Grande and allow assessment of temporal differences in spawning effort.

3.2 Optional Objective Subject to Government Approval to Proceed: Utilize a MEC boat during ideal peak flows to collect higher numbers of RGSM eggs. Examine and pinpoint the flows in which to utilize and deploy the MEC boat device to most effectively collect RGSM eggs.

4.0 Tasks

Task 1—Spawning Monitoring in River Channel

MECs shall be set up using the protocols described in Altenbach et al. (2000) at three (3) locations: the established San Marcial site and the established sites downstream of the Isleta and San Acacia Diversion Dams. A flow-meter shall be attached to the egg collectors to record flows at the time of catch so that a volume of water can be calculated for a catch rate per unit of water measured. Water temperatures shall be taken on the hour throughout the study period.

Timing of Schedule

The period of sampling shall occur during the spring run-off (typically occurring between mid-April and mid-June).

Sampling shall occur once each day for a minimum of four hours total sampling time, and the collecting period shall start approximately April 17, 2027, and continue through approximately June 12, 2027, to cover approximately 50 field days of sampling (including weekends). To ensure that sampling occurs during spring run-off, dates may be adjusted based on run-off forecasts and anticipated environmental conditions.

Materials and Methods

The device used for collecting the eggs shall be a Moore Egg Collector (MEC) (Altenbach 2000). This allows for the efficient and nondestructive collection of large numbers of semi-buoyant fish eggs (i.e. Rio Grande silvery minnow). A flow meter shall be attached to the front of the MEC to accurately quantify the flow (velocity) of water at the time of sampling. Location of the flow meter in the water column shall be recorded. Water temperatures at the MEC location at the time of sampling shall be recorded. Sampling volume is calculated by multiplying the open area of the MEC by the average velocity during the sampling period. The metric for summarizing the sampling can then be expressed as the number of eggs/100 m3, or a catch rate per unit of water volume or catch-per-unit-effort (CPUE). This value can then be extrapolated to the amount of water in the river at the time of sampling and can be used to estimate the number of eggs passing that given point during the sampling period. The metrics provided by the sampling gear must be comparable to previously accomplished work. Current and past year’s data shall be evaluated and analyzed to characterize RGSM spawning over the past several years.

Location

Field samples shall be collected in the Middle Rio Grande at three (3) main river channel sites (Figure 1) as follows: the San Marcial Site and the established sites upstream of the Isleta and San Acacia Diversion Dams.

These sites have been selected since they provide for a more detailed assessment of the species spawning patterns over time (Dudley and Platania 2024).

Optional Task 1—MEC Boat

This task shall begin annually only after the contractor has been provided Government approval to proceed, Not to Exceed (NTE) more than eight (8) uses per field collection time period, typically from mid April to mid June. The main tasks associated with utilizing the MEC boat collector is to:

• Use MEC boat to record the number of eggs collected in areas of the Middle Rio Grande.

o Record number of eggs detected.

o Eggs and data recorded while utilizing the MEC boat should be utilized and incorporated into the analysis and reports required for the “Task 1—Spawning Monitoring in River Channel” field work;

however, the data gathered utilizing the procedures outlined in “Task 1—Spawning Monitoring in River Channel” and MEC boat should be reported separately in order to compare the two methods and to try and pinpoint the flows in which to utilize and deploy the MEC boat device to most effectively collect RGSM eggs.

• Compare the data on eggs collected using the MEC boat to the number of eggs collected utilizing the procedures outlined in “Task 1—Spawning Monitoring in River Channel”.

• Record water temperatures at the MEC boat location at the time of sampling. Compare water temperatures for the same sampling time periods for the MEC boat sites and the river channel sites.

• Provide collected eggs to a propagation facility.

Timing of Schedule

The period of utilizing the MEC boat shall occur within the same overall timeframe as Task 1 (mid-April to mid- June), and NTE more than eight (8) uses per field collection time period. Use of the MEC boat shall be based on annual environmental conditions and at the direction of the Government. A pre-season coordination meeting between the contractor and BOR staff is required prior to utilizing the MEC boat for a field season.

Materials and Methods

A fabricated MEC boat shall be utilized to collect RGSM eggs. A flow-meter shall be attached to or loaded onto the MEC boat to record flows at the time of catch so that a volume of water can be calculated for a catch rate per unit of water measured. Water temperatures shall be recorded during sampling, and discharge shall be recorded from USGS gages, if they are located nearby the MEC boat site. Comparison with data obtained in main river channel sites under Task 1 shall be conducted in order to evaluate ideal conditions in which to utilize the MEC boat.

The device used for collecting the eggs shall be modified Moore Egg Collectors (MECs) (Altenbach 2000), that are engineered onto a boat frame. This allows for the efficient, quantitative, and nondestructive collection of large numbers of semi-buoyant fish eggs. A flow meter shall be attached to or loaded into the MEC boat to accurately quantify the flow (velocity) of water at the time of collection. Location of the flow meter in the water column shall be recorded. Sampling volume is calculated by multiplying the open area of the MEC by the average velocity during the sampling period. The metric for summarizing the sampling can then be expressed as the number of eggs/100 m3, or a catch rate per unit of water volume or catch-per-unit-effort (CPUE).

Location

Field samples shall be collected in the Middle Rio Grande at the three (3) main river channel sites (Figure 1) as defined in the “Task 1—Spawning Monitoring in River Channel” or in areas between the three main river channel sites that show ideal conditions to capture/monitor large densities of RGSM eggs. The contractor shall coordinate with BOR to determine ideal MEC boat sites.

Permits

The recipient must be able to obtain valid collecting permits (State and Federal) for all personnel associated with the collection of silvery minnow eggs. A Notice to Proceed may not be issued if any and all necessary permits are not obtained prior to contract performance (see explanation under 2.0 Scope above). Site permits must be obtained through the Bureau of Reclamation for site specific collecting locations. Failure to obtain proper permits within the needed timeframe may void this contract.

5.0 Deliverables

Task 1: Spawning Monitoring in River Channel

1) The Contractor shall report daily egg catch data in the silvery minnow egg monitoring email list serv maintained by the U.S. Fish and Wildlife Service’s New Mexico Fish and Wildlife Conservation Office.

2) After completion of field work, a draft report shall be written that contains sections describing methods, specific techniques and equipment used (including calibration), standard procedures used for data collection, the data collected, analysis and results. Field data are to be provided with the draft report. The draft report shall be submitted by August 15th to the Reclamation Contracting Officer’s Representative (COR). Note: No management recommendations are required or will be included in the report.

3) The COR and the Science workgroup (or equivalent) of the Middle Rio Grande Endangered Species Collaborative Program (or RIP) will review the draft report and provide comments to the Contractor no later than 30-days after receipt of draft report.

4) After receipt of comments, the Contractor shall either make revisions to the draft report or specify why comments were not incorporated (or partially incorporated) on the standard comment template sheet and provide a final report to the Reclamation COR by September 30th.

5) All data shall be submitted to Reclamation electronically in a usable format specified by Reclamation for import into the Database Management System (DBMS).

6) Statistical analyses shall be performed on the data and at a minimum shall include correlation of egg CPUE with discharge/velocity, and evaluation of water temperature data.

Optional Task 1: MEC Boat

1) The Contractor shall report daily egg catch data gathered by the MEC boat separately from Task 1 in the silvery minnow egg monitoring email list serv maintained by the U.S. Fish and Wildlife Service’s New Mexico Fish and Wildlife Conservation Office.

2) After completion of field work, a draft report shall be written as part of the draft report for Task 1 that contains sections describing methods, specific techniques and equipment used (including calibration), standard procedures used for data collection, the data collected, analysis and results, and a discussion of results. Field data are to be provided with the draft report. The draft report shall be submitted by August 15th as part of the draft report for Task 1 to the Reclamation Contracting Officer’s Representative (COR).

3) The COR shall review the draft report for entrainment monitoring and provide comments to the Contractor no later than 30-days after receipt of draft report.

4) After receipt of comments, the Contractor shall either make revisions to the draft report or specify why comments were not incorporated (or partially incorporated) on the standard comment template sheet, and provide a final report to the Reclamation COR as part of the final report under Task 1 (by September 30th).

5) All data shall be submitted to Reclamation electronically in a usable format specified by Reclamation for import into the Database Management System (DBMS).

6.0 Government-Furnished Property

Historic RGSM spawning monitoring reports, including information on monitoring site locations, are available on the Collaborative Program’s Database Management System (DBMS): http://mrgescp.dbstephens.com/#Home

7.0 Place of Performance

This work shall be performed on the Middle Rio Grande in the Angostura, Isleta, and San Acacia reaches, and at Contractor facilities.

8.0 Period of Performance

This work is scheduled to begin on the date of award and continue for one year, with four option years. Work is expected to begin October 1, 2026 through September 30, 2027 for the base year, with a similar annual schedule for the four option years.

http://mrgescp.dbstephens.com/#Home

9.0 References

Altenbach, C.S., R.K. Dudley, and S.P. Platania. 2000. A new device for collecting drifting semi-buoyant fish eggs. Transactions of the American Fisheries Society 129:296-300.

Dudley, R.K. and S.P. Platania. 2014. Monitoring of the Rio Grande Silvery Minnow Reproductive Effort during 2014 in the Rio Grande and Selected Irrigation Canals. Final Report to Bureau of Reclamation, Albuquerque, New Mexico. 47p.

Dudley, R.K. and S.P. Platania. 2015. Monitoring of the Rio Grande Silvery Minnow Reproductive Effort during 2015 in the Rio Grande and Selected Irrigation Canals. Final Report to Bureau of Reclamation, Albuquerque, New Mexico. 44p.

Dudley, R.K., S.P. Platania, and G.C. White. 2016. Rio Grande Silvery Minnow Reproductive Monitoring during 2016 in the Rio Grande and Selected Irrigation Canals. Final Report to Bureau of Reclamation, Albuquerque, New Mexico. 45p.

Dudley, R.K., S.P. Platania, and G.C. White. 2024. Rio Grande Silvery Minnow Reproductive Monitoring during 2024. Final Report to Bureau of Reclamation, Albuquerque, New Mexico. 97p.Luttrell, G. A., A. A.

Echelle, W. L. Fisher, and D. J. Eisenhour. 1999. Declining status of two species of the Macrhybopsis aestivalis complex (Teleostei: Cyprinidae) in the Arkansas River Basin and related effects of reservoirs as barriers to dispersal. Copeia 1999:981-989.

MRGESCP 2006. Program Monitoring Plan – Appendix A: Rio Grande Fish Community Monitoring. Middle Rio Grande Endangered Species Collaborative Program, Albuquerque, New Mexico. 48p.

Platania, S. P., and C. S. Altenbach. 1998. Reproductive strategies and egg types of seven Rio Grande Basin cyprinids. Copeia 1998:559-569.

U.S. Fish and Wildlife Service. 2016. 2016 Final Biological and Conference Opinion for Bureau of Reclamation, Bureau of Indian Affairs, and Non-Federal Water Management and Maintenance Activities on the Middle Rio Grande, New Mexico. Albuquerque, NM. 192 pp.

U.S. Fish and Wildlife Service. 2010. Rio Grande silvery minnow (Hybognathus amarus) Recovery Plan, First Revision. Albuquerque, NM. viii + 210 pp.

U.S. Fish and Wildlife Service. 2009. Rio Grande Silvery Minnow Genetics Management and Propagation Plan.

Revised February 15, 2009. 31 pp.

Winston, M. R., C. M. Taylor, and J. Pigg. 1991. Upstream extirpation of four minnow species due to damming of a prairie stream. Transactions of the American Fisheries Society 120:98-105.

Figure 1. Map of Middle Rio Grande, New Mexico reproductive monitoring sites (from Dudley et al. 2024).

Sponsored by GSA FAR (48 CFR) 53.110